Mouse CXCL13/BLC/BCA-1 Antibody

Catalog #: MAB470R
1 Citation Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB470R-100
MAB470R-SP
MAB470R-500
MAB470R-01M

Chemotaxis Induced by CXCL13/BLC/BCA-1 and Neutralization by Mouse CXCL13/BLC/BCA-1 Antibody

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CXCL13/BLC/BCA-1 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CXCL13 in ELISAs. In ELISAs, this antibody does not cross-react with recombinant human (rh) CXCL13, rmCXCL11/I‑TAC, rmCXCL9/MIG, or rm CXCL12/SDF‑1 alpha.

Source

Recombinant Monoclonal Rat IgG_2A Clone # 143614R

Purification

Protein A or G purified from cell culture supernatant

Immunogen

E. coli-derived recombinant mouse CXCL13
Ile22-Ala109
Accession # O55038

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Mouse CXCL13/BLC/BCA-1 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Mouse CXCL13/BLC/BCA‑1 Biotinylated Antibody (Catalog # BAF470)

Standard: Recombinant Mouse CXCL13/BLC/BCA-1 Protein (Catalog # 470-BC)

Neutralization

Measured by its ability to neutralize CXCL13/BLC/BCA-1 induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CXCR5. The Neutralization Dose (ND₅₀) is typically 12-30 μg/mL in the presence of 4 μg/mL Recombinant Mouse CXCL13/BLC/BCA-1.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Chemotaxis Induced by CXCL13/BLC/BCA-1 and Neutralization by Mouse CXCL13/BLC/BCA-1 Antibody Recombinant Mouse CXCL13/BLC/BCA-1 (Catalog # 470-BC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL13/BLC/BCA-1 (4 µg/mL) is neutralized (green line) by increasing concentrations of Recombinant Mouse CXCL13/BLC/BCA-1 Monoclonal Antibody (Catalog # MAB470R). The ND₅₀ is typically 12-30 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL13/BLC/BCA-1

CXCL13, also known as B-lymphocyte chemoattractant (BLC) and BCA-1, is a CXC chemokine that is constitutively expressed in secondary lymphoid organs. Mouse CXCL13 cDNA encodes a precursor protein of 109 amino acid residues with a putative leader sequence of 21 residues. Mature mouse CXCL13 shares 64% amino acid sequence similarity with the human protein and 23-34% amino acid sequence identity with other known CXC chemokines. Recombinant or chemically synthesized CXCL13 is a potent chemoattractant for B lymphocytes but not T lymphocytes, monocytes or neutrophils. BLR1, a G protein-coupled receptor originally isolated from Burkitt’s lymphoma cells, has now been shown to be the specific receptor for CXCL13. Among cells of the hematopoietic lineages, the expression of BLR1, now designated CXCR5, is restricted to B lymphocytes and a subpopulation of T helper memory cells. Mice lacking BLR1 have been shown to lack inguinal lymph nodes. These mice were also found to have impaired development of Peyer’s patches and defective formation of primary follicles and germinal centers in the spleen as a result of the inability of B lymphocytes to migrate into B cell areas.

References

Gunn, M.D. et al. (1998) Nature, 391:799.
Legler, D.F. et al. (1998) J. Exp. Med. 187:655.
Forster, R. et al. (1996) Cell 87:1037.

Entrez Gene IDs

10563 (Human); 55985 (Mouse)

Alternate Names

ANGIE; ANGIE2; B cell-attracting chemokine 1; B lymphocyte chemoattractant; BCA1; BCA-1; BCA1B-cell chemoattractant; BCA-1CXC chemokine BLC; B-cell-homing chemokine (ligand for Burkitt's lymphoma receptor-1); BLC; BLCSmall-inducible cytokine B13; BLR1L; B-lymphocyte chemoattractant; chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant); chemokine (C-X-C motif) ligand 13; C-X-C motif chemokine 13; CXCL13; SCYB13; SCYB13B-cell-attracting chemokine 1; small inducible cytokine B subfamily (Cys-X-Cys motif), member 13 (B-cellchemoattractant)

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Citation for Mouse CXCL13/BLC/BCA-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Chemotherapy-induced ileal crypt apoptosis and the ileal microbiome shape immunosurveillance and prognosis of proximal colon cancer
Authors: MP Roberti, S Yonekura, CPM Duong, M Picard, G Ferrere, M Tidjani Al, C Rauber, V Iebba, CHK Lehmann, L Amon, D Dudziak, L Derosa, B Routy, C Flament, C Richard, R Daillère, A Fluckiger, I Van Seunin, M Chamaillar, A Vincent, S Kourula, P Opolon, P Ly, E Pizzato, S Becharef, J Paillet, C Klein, F Marliot, F Pietranton, S Benoist, JY Scoazec, P Dartigues, A Hollebecqu, D Malka, F Pagès, J Galon, I Gomperts B, P Lepage, B Ryffel, D Raoult, A Eggermont, T Vanden Ber, F Ghiringhel, P Vandenabee, G Kroemer, L Zitvogel
Nat. Med., 2020-05-25;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo