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Mouse CXCL13/BLC/BCA-1 Antibody

Catalog #: AF470
38 Citations Datasheet / COA / SDS
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AF470
AF470-SP
Chemotaxis Induced by CXCL13/BLC/BCA‑1 and Neutralization by Mouse CXCL13/BLC/BCA‑1 Antibody.
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Citations (38)
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Mouse CXCL13/BLC/BCA-1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CXCL13/BLC/BCA‑1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) CXCL13 and rhGCP-2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse CXCL13/BLC/BCA‑1
Ile22-Ala109
Accession # Q3U1E8
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Purity
Immunogen affinity purified

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse CXCL13/BLC/BCA‑1 (Catalog # 470-BC)
Immunohistochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize CXCL13/BLC/BCA‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR5. The Neutralization Dose (ND50) is typically 20-50 µg/mL in the presence of 4 µg/mL Recombinant Mouse CXCL13/BLC/BCA‑1.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Chemotaxis Induced by CXCL13/BLC/BCA‑1 and Neutralization by Mouse CXCL13/BLC/BCA‑1 Antibody. View Larger

Chemotaxis Induced by CXCL13/BLC/BCA‑1 and Neutralization by Mouse CXCL13/BLC/BCA‑1 Antibody. Recombinant Mouse CXCL13/BLC/BCA-1 (Catalog # 470-BC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin staining. Chemotaxis elicited by Recombinant Mouse CXCL13/BLC/BCA-1 (4 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CXCL13/ BLC/BCA-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF470). The ND50 is typically 20-50 µg/mL.

Immunohistochemistry CXCL13/BLC/BCA-1 antibody in Mouse Intestine by Immunohistochemistry (IHC-Fr). View Larger

CXCL13/BLC/BCA‑1 in Mouse Intestine. CXCL13/BLC/BCA-1 was detected in perfusion fixed frozen sections of mouse intestine (Peyer's patch) using Goat Anti-Mouse CXCL13/BLC/BCA-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF470) at 15 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to endothelial cells. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL13/BLC/BCA-1

CXCL13, also known as B-lymphocyte chemoattractant (BLC), is a CXC chemokine that is constitutively expressed in secondary lymphoid organs. Mouse BCA-1 cDNA encodes a precursor protein of 109 amino acid residues with a putative leader sequence of 21 residues. Mature mouse BCA-1 shares 64% amino acid sequence similarity with the human protein and 23‑34% amino acid sequence identity with other known CXC chemokines. Recombinant or chemically synthesized BCA-1 is a potent chemoattractant for B lymphocytes but not T lymphocytes, monocytes or neutrophils. BLR1, a G protein-coupled receptor originally isolated from Burkitt’s lymphoma cells, has now been shown to be the specific receptor for BCA-1. Among cells of the hematopoietic lineages, the expression of BLR1, now designated CXCR5, is restricted to B lymphocytes and a subpopulation of T helper memory cells. Mice lacking BLR1 have been shown to lack inguinal lymph nodes. These mice were also found to have impaired development of Peyer’s patches and defective formation of primary follicles and germinal centers in the spleen as a result of the inability of B lymphocytes to migrate into B cell areas.

References
  1. Gunn, M.D. et al. (1998) Nature, 391:799.
  2. Legler, D.F. et al. (1998) J. Exp. Med. 187:655.
  3. Forster, R. et al. (1996) Cell 87:1037.
Entrez Gene IDs
10563 (Human); 55985 (Mouse)
Alternate Names
ANGIE; ANGIE2; B cell-attracting chemokine 1; B lymphocyte chemoattractant; BCA1; BCA-1; BCA1B-cell chemoattractant; BCA-1CXC chemokine BLC; B-cell-homing chemokine (ligand for Burkitt's lymphoma receptor-1); BLC; BLCSmall-inducible cytokine B13; BLR1L; B-lymphocyte chemoattractant; chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant); chemokine (C-X-C motif) ligand 13; C-X-C motif chemokine 13; CXCL13; SCYB13; SCYB13B-cell-attracting chemokine 1; small inducible cytokine B subfamily (Cys-X-Cys motif), member 13 (B-cellchemoattractant)

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Citations for Mouse CXCL13/BLC/BCA-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

38 Citations: Showing 1 - 10
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  1. Venous-plexus-associated lymphoid hubs support meningeal humoral immunity
    Authors: Fitzpatrick, Z;Ghabdan Zanluqui, N;Rosenblum, JS;Tuong, ZK;Lee, CYC;Chandrashekhar, V;Negro-Demontel, ML;Stewart, AP;Posner, DA;Buckley, M;Allinson, KSJ;Mastorakos, P;Chittiboina, P;Maric, D;Donahue, D;Helmy, A;Tajsic, T;Ferdinand, JR;Portet, A;Peñalver, A;Gillman, E;Zhuang, Z;Clatworthy, MR;McGavern, DB;
    Nature
    Species: Murine polyomavirus strain A3
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  2. CXCL13/CXCR5 signalling is pivotal to preserve motor neurons in amyotrophic lateral sclerosis
    Authors: MC Trolese, A Mariani, M Terao, M de Paola, P Fabbrizio, F Sironi, M Kurosaki, S Bonanno, S Marcuzzo, P Bernasconi, F Trojsi, E Aronica, C Bendotti, G Nardo
    EBioMedicine, 2020-11-09;62(0):103097.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. CDK4/6 inhibition promotes immune infiltration in ovarian cancer and synergizes with PD-1 blockade in a B cell-dependent manner
    Authors: QF Zhang, J Li, K Jiang, R Wang, JL Ge, H Yang, SJ Liu, LT Jia, L Wang, BL Chen
    Theranostics, 2020-08-25;10(23):10619-10633.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Migration Assay
  4. Dietary lipids accumulate in macrophages and stromal cells and change the microarchitecture of mesenteric lymph nodes
    Authors: K Streich, M Smoczek, J Hegermann, O Dittrich-B, M Bornemann, A Siebert, A Bleich, M Buettner
    J Adv Res, 2020-04-29;24(0):291-300.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Repression of the Type I Interferon pathway underlies MYC & KRAS-dependent evasion of NK & B cells in Pancreatic Ductal Adenocarcinoma
    Authors: N Muthalagu, T Monteverde, X Raffo-Irao, R Wiesheu, D Whyte, A Hedley, S Laing, B Kruspig, R Upstill-Go, R Shaw, S Neidler, C Rink, SA Karim, K Gyuraszova, C Nixon, W Clark, AV Biankin, LM Carlin, SB Coffelt, OJ Sansom, JP Morton, DJ Murphy
    Cancer Discov, 2020-03-21;0(0):.
    Species: Mouse
    Sample Types:
    Applications: In Vivo
  6. Foliate Lymphoid Aggregates as Novel Forms of Serous Lymphocyte Entry Sites of Peritoneal B Cells and High-Grade B Cell Lymphomas
    Authors: X Jia, F Gábris, Ó Jacobsen, G Bedics, B Botz, Z Helyes, Z Kellermaye, D Vojkovics, G Berta, N Nagy, Z Jakus, P Balogh
    J. Immunol., 2019-11-25;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. IL-4R?-Expressing B Cells Are Required for CXCL13 Production by Fibroblastic Reticular Cells
    Authors: LK Dubey, B Ludewig, SA Luther, NL Harris
    Cell Rep, 2019-05-21;27(8):2442-2458.e5.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-F
  8. The female-biased factor VGLL3 drives cutaneous and systemic autoimmunity
    Authors: AC Billi, M Gharaee-Ke, J Fullmer, LC Tsoi, BD Hill, D Gruszka, J Ludwig, X Xing, S Estadt, SJ Wolf, SM Rizvi, CC Berthier, JB Hodgin, MA Beamer, MK Sarkar, Y Liang, R Uppala, S Shao, C Zeng, PW Harms, ME Verhaegen, JJ Voorhees, F Wen, NL Ward, AA Dlugosz, JM Kahlenberg, JE Gudjonsson
    JCI Insight, 2019-04-18;4(8):.
    Species: Mouse
    Sample Types: Whole Cells
  9. Origin and differentiation trajectories of fibroblastic reticular cells in the splenic white pulp
    Authors: HW Cheng, L Onder, M Novkovic, C Soneson, M Lütge, N Pikor, E Scandella, MD Robinson, JI Miyazaki, A Tersteegen, U Sorg, K Pfeffer, T Rülicke, T Hehlgans, B Ludewig
    Nat Commun, 2019-04-15;10(1):1739.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. Human Adipose Tissue-Derived Mesenchymal Stem Cells Attenuate Atopic Dermatitis by Regulating the Expression of MIP-2, miR-122a-SOCS1 Axis, and Th1/Th2 Responses
    Authors: M Kim, SH Lee, Y Kim, Y Kwon, Y Park, HK Lee, HS Jung, D Jeoung
    Front Pharmacol, 2018-11-06;9(0):1175.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. Inhaled TLR9 Agonist Renders Lung Tumors Permissive to PD-1 Blockade by Promoting Optimal CD4+ and CD8+ T cell Interplay
    Authors: M Gallotta, H Assi, É Degagné, SK Kannan, RL Coffman, C Guiducci
    Cancer Res., 2018-06-26;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  12. Genetic ablation of histone deacetylase 2 leads to lung cellular senescence and lymphoid follicle formation in COPD/emphysema
    Authors: IK Sundar, K Rashid, J Gerloff, J Rangel-Mor, D Li, I Rahman
    FASEB J., 2018-04-09;0(0):fj201701518R.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  13. Leukocyte-endothelial cell interaction is enhanced in podocalyxin-deficient mice
    Authors: G Porras, MS Ayuso, C González-M
    Int. J. Biochem. Cell Biol., 2018-03-28;99(0):72-79.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  14. Hyperactive gp130/STAT3-driven gastric tumourigenesis promotes submucosal tertiary lymphoid structure development
    Authors: DG Hill, L Yu, H Gao, JJ Balic, A West, H Oshima, L McLeod, M Oshima, A Gallimore, K D'Costa, PS Bhathal, W Sievert, RL Ferrero, BJ Jenkins, GW Jones
    Int. J. Cancer, 2018-02-19;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  15. Application of light sheet microscopy for qualitative and quantitative analysis of bronchus-associated lymphoid tissue in mice
    Authors: DT Mzinza, H Fleige, K Laarmann, S Willenzon, J Ristenpart, J Spanier, G Sutter, U Kalinke, P Valentin-W, R Förster
    Cell. Mol. Immunol., 2018-02-12;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  16. Expression of the B cell differentiation factor BAFF and chemokine CXCL13 in a murine model of Respiratory Syncytial Virus infection
    Authors: W Alturaiki, AJ McFarlane, K Rose, R Corkhill, PS McNamara, J Schwarze, BF Flanagan
    Cytokine, 2018-02-01;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  17. Role of SIRP? in Homeostatic Regulation of T Cells and Fibroblastic Reticular Cells in the Spleen
    Authors: D Respatika, Y Saito, K Washio, S Komori, T Kotani, H Okazawa, Y Murata, T Matozaki
    Kobe J Med Sci, 2017-08-10;63(1):E22-E29.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  18. Ageing adversely affects the migration and function of marginal zone B cells
    Authors: VM Turner, NA Mabbott
    Immunology, 2017-05-04;151(3):349-362.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  19. Pneumocystis-Driven Inducible Bronchus-Associated Lymphoid Tissue Formation Requires Th2 and Th17 Immunity
    Authors: T Eddens, W Elsegeiny, ML Garcia-Her, P Castillo, G Trevejo-Nu, K Serody, BT Campfield, SA Khader, K Chen, J Rangel-Mor, JK Kolls
    Cell Rep, 2017-03-28;18(13):3078-3090.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  20. Brain inflammation is accompanied by peripheral inflammation in Cstb (-/-) mice, a model for progressive myoclonus epilepsy
    J Neuroinflammation, 2016-11-28;13(1):298.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  21. Hif1a Deletion Reveals Pro-Neoplastic Function of B Cells in Pancreatic Neoplasia
    Cancer Discov, 2015-12-29;6(3):256-69.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC-P, Western Blot
  22. CXCL13 antibody for the treatment of autoimmune disorders.
    Authors: Klimatcheva E, Pandina T, Reilly C, Torno S, Bussler H, Scrivens M, Jonason A, Mallow C, Doherty M, Paris M, Smith E, Zauderer M
    BMC Immunol, 2015-02-12;16(0):6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  23. Stromal cells as trend-setters for cells migrating into the lymph node.
    Authors: Buettner M, Dittrich-Breiholz O, Falk C, Lochner M, Smoczek A, Menzel F, Bornemann M, Bode U
    Mucosal Immunol, 2014-11-05;8(3):640-9.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  24. IL-17-induced CXCL12 recruits B cells and induces follicle formation in BALT in the absence of differentiated FDCs.
    Authors: Fleige H, Ravens S, Moschovakis G, Bolter J, Willenzon S, Sutter G, Haussler S, Kalinke U, Prinz I, Forster R
    J Exp Med, 2014-03-24;211(4):643-51.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  25. Anti-CXCL13 antibody can inhibit the formation of gastric lymphoid follicles induced by Helicobacter infection.
    Authors: Yamamoto, K, Nishiumi, S, Yang, L, Klimatcheva, E, Pandina, T, Takahashi, S, Matsui, H, Nakamura, M, Zauderer, M, Yoshida, M, Azuma, T
    Mucosal Immunol, 2014-03-19;7(5):1244-54.
    Species: Mouse
    Sample Types: Cell Lysates, In Vivo, Whole Tissue
    Applications: IHC, Neutralization, Western Blot
  26. CLEC-2 is required for development and maintenance of lymph nodes.
    Authors: Benezech C, Nayar S, Finney B, Withers D, Lowe K, Desanti G, Marriott C, Watson S, Caamano J, Buckley C, Barone F
    Blood, 2014-02-14;123(20):3200-7.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  27. Repetitive hypoxic preconditioning induces an immunosuppressed B cell phenotype during endogenous protection from stroke.
    Authors: Monson N, Ortega S, Ireland S, Meeuwissen A, Chen D, Plautz E, Shubel E, Kong X, Li M, Freriks L, Stowe A
    J Neuroinflammation, 2014-01-31;11(0):22.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  28. Identification of a new stromal cell type involved in the regulation of inflamed B cell follicles.
    Authors: Mionnet C, Mondor I, Jorquera A, Loosveld M, Maurizio J, Arcangeli M, Ruddle N, Nowak J, Aurrand-Lions M, Luche H, Bajenoff M
    PLoS Biol, 2013-10-01;11(10):e1001672.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC
  29. CXCL13 is elevated in Sjogren's syndrome in mice and humans and is implicated in disease pathogenesis.
    Authors: Kramer J, Klimatcheva E, Rothstein T
    J Leukoc Biol, 2013-07-31;94(5):1079-89.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  30. IL-23 is required for long-term control of Mycobacterium tuberculosis and B cell follicle formation in the infected lung.
    Authors: Khader SA, Guglani L, Rangel-Moreno J
    J. Immunol., 2011-10-14;187(10):5402-7.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  31. The sensing of environmental stimuli by follicular dendritic cells promotes immunoglobulin A generation in the gut.
    Authors: Suzuki K, Maruya M, Kawamoto S
    Immunity, 2010-07-23;33(1):71-83.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  32. Comprehensive assessment of chemokine expression profiles by flow cytometry.
    Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
    J. Clin. Invest., 2010-02-08;120(3):907-23.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  33. Salmonella disrupts lymph node architecture by TLR4-mediated suppression of homeostatic chemokines.
    Authors: St John AL, Abraham SN
    Nat. Med., 2009-10-25;15(11):1259-65.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  34. Role of lymphotoxin-alpha in cigarette smoke-induced inflammation and lymphoid neogenesis.
    Authors: Demoor T, Bracke KR, Maes T, Vandooren B, Elewaut D, Pilette C, Joos GF, Brusselle GG
    Eur. Respir. J., 2009-01-22;34(2):405-16.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  35. Lymphotoxin beta receptor signaling promotes tertiary lymphoid organogenesis in the aorta adventitia of aged ApoE-/- mice.
    Authors: Grabner R, Lotzer K, Dopping S, Hildner M, Radke D, Beer M, Spanbroek R, Lippert B, Reardon CA, Getz GS, Fu YX, Hehlgans T, Mebius RE, van der Wall M, Kruspe D, Englert C, Lovas A, Hu D, Randolph GJ, Weih F, Habenicht AJ
    J. Exp. Med., 2009-01-12;206(1):233-48.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  36. The chemokine decoy receptor M3 blocks CC chemokine ligand 2 and CXC chemokine ligand 13 function in vivo.
    Authors: Martin AP, Canasto-Chibuque C, Shang L, Rollins BJ, Lira SA
    J. Immunol., 2006-11-15;177(10):7296-302.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  37. Regulation of B1 cell migration by signals through Toll-like receptors.
    Authors: Ha SA, Tsuji M, Suzuki K, Meek B, Yasuda N, Kaisho T, Fagarasan S
    J. Exp. Med., 2006-10-23;203(11):2541-50.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  38. Suppression of established experimental autoimmune encephalomyelitis and formation of meningeal lymphoid follicles by lymphotoxin beta receptor-Ig fusion protein.
    Authors: Columba-Cabezas S, Griguoli M, Rosicarelli B, Magliozzi R, Ria F, Serafini B, Aloisi F
    J. Neuroimmunol., 2006-07-25;179(1):76-86.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC

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