Mouse CXCL16 Antibody

Name: Mouse CXCL16 Antibody
Brand: R&D Systems
SKU: MAB503
Rating: 5 (1 reviews)

Catalog #: MAB503
7 Citations 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB503-SP
MAB503-500
MAB503-100

Chemotaxis Induced by CXCL16 and Neutralization by Mouse CXCL16 Antibody.

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All CXCL16 Antibodies

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Citations (7)

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Reviews (1)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CXCL16 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CXCL16 in ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CXCL1, 2, 3, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, recombinant mouse CXCL1, 2, 6, 9, 10, 11, 12, 13, 14, 15, recombinant rat CXCL1, 2, 3, 5, or recombinant porcine CXCL8 is observed.

Source

Monoclonal Rat IgG_2A Clone # 142417

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse CXCL16
Asn27-Pro114
Accession # Q8BSU2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse CXCL16 Chemokine Domain (Catalog # 503-CX) under non-reducing conditions only

Mouse CXCL16 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Mouse CXCL16 Biotinylated Antibody (Catalog # BAF503)

Standard: Recombinant Mouse CXCL16 Protein (Catalog # 503-CX)

Neutralization

Measured by its ability to neutralize CXCL16-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CXCR6. Matloubian, M. et al. (2000) Nature Immunol. 1:298. The Neutralization Dose (ND₅₀) is typically 0.15-0.6 µg/mL in the presence of 7.5 ng/mL Recombinant Mouse CXCL16 Chemokine Domain.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Chemotaxis Induced by CXCL16 and Neutralization by Mouse CXCL16 Antibody. Recombinant Mouse CXCL16 Chemokine Domain (Catalog # 503-CX) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CXCR6 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL16 Chemokine Domain (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CXCL16 Monoclonal Antibody (Catalog # MAB503). The ND₅₀ is typically 0.15-0.6 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL16

Mouse CXCL16 (CXC chemokine 16) is a non-ELR motif-containing CXC chemokine with a transmembrane domain. CX3CL1/Fractalkine andCXCL16 are the only two transmembrane chemokines within the superfamily. Mouse CXCL16 cDNA encodes a 246 amino acid (aa) precursor protein with a putative 26 aa signal peptide, an 88 aa chemokine domain, an 87 aa mucin-like spacer region, a 22 aa transmembrane domain, and a 23 aa cytoplasmic tail. Mouse and human CXCL16 share 49% aa sequence identity. Mouse CXCL16 is produced by dendritic cells in lymphoid organ T cell zones and bycells in the splenic red pulp both as membrane-bound and soluble forms. Based on northern blot analysis, CXCL16 is also expressed in some non-lymphoid tissues such as lung, small intestine and kidney. The receptor for CXCL16 has been identified as CXCR6 (previously called Bonzo, STRL33 or TYMSTR), which is also a coreceptor for HIV entry. CXCR6 is expressed on naive CD8 cells, natural killer T cells and activated CD8 and CD4 T cells.

Entrez Gene IDs

58191 (Human); 66102 (Mouse)

Alternate Names

chemokine (C-X-C motif) ligand 16; CXC chemokine ligand 16; CXCL16; CXCLG16; Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein; SCYB16; Small-inducible cytokine B16; SRPSOXC-X-C motif chemokine 16; SR-PSOXTransmembrane chemokine CXCL16

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse CXCL16 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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LSD1 drives intestinal epithelial maturation and controls small intestinal immune cell composition independent of microbiota in a murine model
Authors: Díez-Sánchez, A;Lindholm, HT;Vornewald, PM;Ostrop, J;Yao, R;Single, AB;Marstad, A;Parmar, N;Shaw, TN;Martín-Alonso, M;Oudhoff, MJ;
Nature communications
Species: Viral
Sample Types: In Vivo
Applications: Neutralization
Targeting cardiomyocyte ADAM10 ectodomain shedding promotes survival early after myocardial infarction
Authors: E Klapproth, A Witt, P Klose, J Wiedemann, N Vavilthota, SR Künzel, S Kämmerer, M Günscht, D Sprott, M Lesche, F Rost, A Dahl, E Rauch, L Kattner, S Weber, P Mirtschink, I Kopaliani, K Guan, K Lorenz, P Saftig, M Wagner, A El-Armouch
Nature Communications, 2022-12-10;13(1):7648.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Inflammation triggers ILC3 patrolling of the intestinal barrier
Authors: A Jarade, Z Garcia, S Marie, A Demera, I Prinz, P Bousso, JP Di Santo, N Serafini
Nature Immunology, 2022-08-23;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo
Phosphodiesterase 5 (PDE5) Is Highly Expressed in Cancer-Associated Fibroblasts and Enhances Breast Tumor Progression
Authors: S Catalano, S Panza, G Augimeri, C Giordano, R Malivindi, L Gelsomino, S Marsico, F Giordano, B Gy?rffy, D Bonofiglio, S Andò, I Barone
Cancers (Basel), 2019-11-06;11(11):.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization
Response patterns of cytokines/chemokines in two murine strains after irradiation.
Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
Cytokine, 2012-01-25;58(2):169-77.
Species: Mouse
Sample Types: Plasma
Applications: Luminex Development
Excreted urinary mediators in an animal model of experimental immune nephritis with potential pathogenic significance.
Authors: Wu T, Xie C, Bhaskarabhatla M, Yan M, Leone A, Chen SS, Zhou XJ, Putterman C, Mohan C
Arthritis Rheum., 2007-03-01;56(3):949-59.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization
A disintegrin and metalloproteinase 10-mediated cleavage and shedding regulates the cell surface expression of CXC chemokine ligand 16.
Authors: Gough PJ, Garton KJ, Wille PT, Rychlewski M, Dempsey PJ, Raines EW
J. Immunol., 2004-03-15;172(6):3678-85.
Species: Mouse
Sample Types: Cell Culture Supernates, Whole Cells
Applications: ELISA Development, Flow Cytometry