Mouse CXCR3 Antibody Summary
Met1-Leu367
Accession # AAC40163
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
![Detection of CXCR3 antibody in Mouse Splenocytes antibody by Flow Cytometry. Detection of CXCR3 antibody in Mouse Splenocytes antibody by Flow Cytometry.](https://resources.rndsystems.com/images/datasheets/antibody/CXCR3_MAB1685_Flow_Cytometry_20061.jpg)
Detection of CXCR3 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC-conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Rat Anti-Mouse CXCR3 Monoclonal Antibody (Catalog # MAB1685) or (B) Rat IgG2AIsotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.
![Detection of Mouse CXCR3 by Flow Cytometry Detection of Mouse CXCR3 by Flow Cytometry](https://resources.rndsystems.com/images/datasheets/antibody/mab1685_mouse-cxcr3-mab-clone-220803-flow-cytometry-412024133017..jpg)
Detection of Mouse CXCR3 by Flow Cytometry Priming of pik3cg−/− CD4+ T cells is reduced during EAE.(A) Frequencies of CD4+ cells in draining LN that are CD69+ on day 9 post-immunisation for EAE as determined by flow cytometry. A representative histogram overlay gating on CD4+ cells is shown (filled = isotype control on WT, solid line = anti-CD69 on WT, dotted line = anti-CD69 on pik3cg−/−) (B) In vivo proliferation of CD4+ cells, measured by BrdU incorporation, is reduced in pik3cg−/− mice at day 9 post-immunization for EAE. (n = 6 mice per group). Representative dot plots gating on CD4+ cells are shown. (C) Expression of chemokine receptors by CD4+ T cells indicative of T cell activation was determined by flow cytometry. Representative histogram overlays showing expression of CCR7, CCR6 and CXCR3 are shown (filled = isotype control, solid line = WT, dotted line = pik3cg−/−). (D) Expression of CD62L, CD49d and PSGL-1 by CD4+ cells from spleen of day 9 immunised mice (n = 3 mice per group). All data shown are mean ± s.e.m. (*, p<0.05). Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0045095), licensed under a CC-BY license. Not internally tested by R&D Systems.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCR3
CXCR3 is a G protein coupled chemokine receptor that binds the alpha chemokines MIG (CXCL9), IP-10 (CXCL10) and I-TAC (CXCL11). CXCR3 is expressed on activated T cells, B cells and NK cells.
Product Datasheets
Citations for Mouse CXCR3 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
14
Citations: Showing 1 - 10
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PI3K gamma drives priming and survival of autoreactive CD4(+) T cells during experimental autoimmune encephalomyelitis
Authors: Iain Comerford, Wendel Litchfield, Ervin Kara, Shaun R. McColl
PLoS ONE
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Rapid Internalization and Nuclear Translocation of CCL5 and CXCL4 in Endothelial Cells
Authors: A Dickhout, DM Kaczor, ACA Heinzmann, SLN Brouns, JWM Heemskerk, MAMJ van Zandvo, RR Koenen
International Journal of Molecular Sciences, 2021-07-08;22(14):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Quantitative single-cell interactomes in normal and virus-infected mouse lungs
Authors: MP Cain, BJ Hernandez, J Chen
Dis Model Mech, 2020-06-26;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Classical Type 1 Dendritic Cells Dominate Priming of Th1 Responses to Herpes Simplex Virus Type 1 Skin Infection
Authors: CM Harpur, Y Kato, ST Dewi, S Stankovic, DN Johnson, S Bedoui, PG Whitney, MH Lahoud, I Caminschi, WR Heath, AG Brooks, T Gebhardt
J. Immunol., 2018-12-31;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
CD4 T cell epitope specificity and cytokine potential are preserved as cells transition from the lung vasculature to lung tissue following influenza infection
Authors: A DiPiazza, N Laniewski, A Rattan, DJ Topham, J Miller, AJ Sant
J. Virol., 2018-06-13;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
The histone demethylase UTX regulates the lineage-specific epigenetic program of invariant natural killer T cells
Nat. Immunol, 2016-12-19;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
IL-10+ Innate-like B Cells Are Part of the Skin Immune System and Require ?4?1 Integrin To Migrate between the Peritoneum and Inflamed Skin
Authors: SA Geherin, D Gómez, RA Glabman, G Ruthel, A Hamann, GF Debes
J. Immunol, 2016-02-05;196(6):2514-25.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
NKG2D ligand overexpression in lupus nephritis correlates with increased NK cell activity and differentiation in kidneys but not in the periphery.
Authors: Spada R, Rojas J, Perez-Yague S, Mulens V, Cannata-Ortiz P, Bragado R, Barber D
J Leukoc Biol, 2015-01-12;97(3):583-98.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
IL-21 promotes late activator APC-mediated T follicular helper cell differentiation in experimental pulmonary virus infection.
Authors: Yoo J, Braciale T
PLoS ONE, 2014-09-24;9(9):e105872.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Flow Cytometry -
Early differentiated CD138(high) MHCII+ IgG+ plasma cells express CXCR3 and localize into inflamed kidneys of lupus mice.
Authors: Lacotte S, Decossas M, Le Coz C, Brun S, Muller S, Dumortier H
PLoS ONE, 2013-03-08;8(3):e58140.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
T cells dampen innate immune responses through inhibition of NLRP1 and NLRP3 inflammasomes.
Authors: Guarda G, Dostert C, Staehli F, Cabalzar K, Castillo R, Tardivel A, Schneider P, Tschopp J
Nature, 2009-06-03;460(7252):269-73.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
L-selectin-negative CCR7- effector and memory CD8+ T cells enter reactive lymph nodes and kill dendritic cells.
Authors: Guarda G, Hons M, Soriano SF, Huang AY, Polley R, Martin-Fontecha A, Stein JV, Germain RN, Lanzavecchia A, Sallusto F
Nat. Immunol., 2007-05-27;8(7):743-52.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Temporal dissection of T-bet functions.
Authors: Matsuda JL, George TC, Hagman J, Gapin L
J. Immunol., 2007-03-15;178(6):3457-65.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
CXCR3-dependent recruitment of antigen-specific T lymphocytes to the liver during murine cytomegalovirus infection.
Authors: Hokeness KL, Deweerd ES, Munks MW, Lewis CA, Gladue RP, Salazar-Mather TP
J. Virol., 2006-11-15;81(3):1241-50.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
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