Mouse DCAR/CLEC4B Antibody

Catalog #: MAB2757
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2757
MAB2757-SP

All DCAR/CLEC4B Antibodies

Product Details

Citations (2)

Supplemental Products

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse DCAR/CLEC4B Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse DCAR/CLEC4B in direct ELISAs.

Source

Monoclonal Rat IgG_2A Clone # 349214

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse DCAR/CLEC4B
Gln42-Leu209
Accession # AAR13070

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

Mouse bone marrow‑derived dendritic cells

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DCAR/CLEC4B

Dendritic Cell immuno-Activating Receptor alpha isoform (DCAR) is a type II membrane protein belonging to the C-type lectin domain family and is designated CLEC4B. Two isoforms of DCAR exist, a 209 amino acid (aa) residue alpha isoform and a 176 aa form with a 33 aa deletion at the membrane proximal region of the extracellular domain. The DCAR extracellular domain contains a carbohydrate-recognition domain (CRD) that shares 91% amino acid sequence identity with the CRD of DCIR/CLEC4A. The DCAR intracellular domain is very short and lacks the ITIM motif found in DCIR. DCAR and DCIR are considered paired immunoregulatory receptors where DCAR activates through the ITAM of its associated adaptor molecule FcR gamma.

Long Name

Dendritic Cell Immunoreceptor

Entrez Gene IDs

69810 (Mouse)

Alternate Names

Aplra2; CLEC4B; CLECSF3; DCAR; LSLCL; P47; SCGF

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse DCAR/CLEC4B Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Structural insight into the recognition of pathogen-derived phosphoglycolipids by C-type lectin receptor DCAR
Authors: Z Omahdi, Y Horikawa, M Nagae, K Toyonaga, A Imamura, K Takato, T Teramoto, H Ishida, Y Kakuta, S Yamasaki
J. Biol. Chem., 2020-03-05;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Dectin-2 recognition of house dust mite triggers cysteinyl leukotriene generation by dendritic cells.
Authors: Barrett NA, Maekawa A, Rahman OM, Austen KF, Kanaoka Y
J. Immunol., 2009-01-15;182(2):1119-28.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry