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Mouse DLL4 Antibody

Catalog #: AF1389
32 Citations Datasheet / COA / SDS
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AF1389
AF1389-SP
Detection of Mouse DLL4 by Western Blot.
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Product Details
Citations (32)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse DLL4 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse DLL4 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross‑reactivity with recombinant human DLL4 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse DLL4
Ser28-Pro525
Accession # BAB18580
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse DLL4 antibody by Western Blot. View Larger

Detection of Mouse DLL4 by Western Blot. Western blot shows lysates of bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Mouse DLL4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1389) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for DLL4 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry DLL4 antibody in bEnd by Immunocytochemistry (ICC).3 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

DLL4 in bEnd.3 Mouse Cell Line. DLL4 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Goat Anti-Mouse DLL4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1389) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry DLL4 antibody in Mouse Embryo by Immunohistochemistry (IHC-P). View Larger

DLL4 in Mouse Embryo. DLL4 was detected in immersion fixed paraffin-embedded sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse DLL4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1389) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to developing vasculature. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DLL4

Delta-Like protein 4 (DLL4) is a type I membrane protein belonging to the Delta/Serrate/Lag2 (DSL) family of Notch ligands (1). Notch signaling is an evolutionarily conserved pathway that controls cell fate and is required in multiple developmental processes including vascular development, hematopoiesis, somatogenesis, myogenesis, and neurogenesis (2-4). Dysregulation in the Notch pathway is associated with various human diseases. In mammals, four Notch homologs (Notch 1 to 4) and five ligands (DLL 1, 3 and 4, Jagged 1 and 2) have been identified. Notch ligands are transmembrane proteins with a DSL motif necessary for Notch binding, tandem EGF repeats, a transmembrane region and a short intracellular domain (ICD). Notch ligands are categorized into two subfamilies based on the presence of an extracellular cysteine-rich domain and insertions that interrupt some EGF repeats in the Jagged but not the Delta ligand family. Interactions of Notch receptors with their ligands results in reciprocal Regulated Intramembrane Proteolysis (RIP) (4). RIP is a mechanism for transmembrane signal transduction that involves the sequential processing by A Disintegrin Metalloprotease (ADAM) and then by Presenilin/ gamma -Ssecretase, resulting in shedding of the extracellular domains and the generation of the soluble ICD signaling fragments, respectively. The Notch ICD translocates to the nucleus and interacts with transcriptional coactivators, resulting in the transcription of target genes. The ICDs of the Notch ligands have also been shown to translocate to the nucleus where they may have a signaling function (5, 6). DLL4 is expressed highly and selectively within the arterial endothelium and has been shown to function as a ligand for Notch 1 and Notch 4. Human and mouse DLL4 share 86% amino acid sequence identity (1).

References
  1. Shutter, J.R. et al. (2000) Genes Dev. 14:1313.
  2. Iso, Tatsuya et al. (2002) Arterioscler. Thromb. Vasc. Biol. 23:543.
  3. Walker, L. et al. (2001) Stem Cells 19:543.
  4. Baron, M. (2002) Semin. Cell Dev. Biol. 14:113.
  5. Ikeuchi, T. and S.S. Sisodia (2003) J. Biol. Chem. 278:7751.
  6. Bland, C.E. et al. (2003) J. Biol. Chem. 278:13607.
Long Name
Delta-like 4
Entrez Gene IDs
54567 (Human); 54485 (Mouse); 100152163 (Porcine)
Alternate Names
Delta 4 precursor; delta 4; delta ligand 4; delta4; delta-like 4 (Drosophila); delta-like 4 homolog (Drosophila); delta-like 4 homolog; delta-like 4 protein; delta-like protein 4; DLL4; Drosophila Delta homolog 4; hdelta2; MGC126344; notch ligand delta-2; notch ligand DLL4

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Citations for Mouse DLL4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

32 Citations: Showing 1 - 10
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  1. PI(4,5)P2-dependent regulation of endothelial tip cell specification contributes to angiogenesis
    Authors: EM Davies, R Gurung, KQ Le, KTT Roan, RP Harvey, GM Mitchell, Q Schwarz, CA Mitchell
    Science Advances, 2023-03-31;9(13):eadd6911.
    Species: Mouse
    Sample Types: Embryo
    Applications: IHC
  2. Bacillus subtilis programs the differentiation of intestinal secretory lineages to inhibit Salmonella infection
    Authors: Q Hou, J Jia, J Lin, L Zhu, S Xie, Q Yu, Y Li
    Cell Reports, 2022-09-27;40(13):111416.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Gatekeeping role of Nf2/Merlin in vascular tip EC induction through suppression of VEGFR2 internalization
    Authors: JH Bae, MJ Yang, SH Jeong, J Kim, SP Hong, JW Kim, YH Kim, GY Koh
    Science Advances, 2022-06-10;8(23):eabn2611.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Induction of osteogenesis by bone-targeted Notch activation
    Authors: C Xu, VV Dinh, K Kruse, HW Jeong, EC Watson, S Adams, F Berkenfeld, M Stehling, SJ Rasouli, R Fan, R Chen, I Bedzhov, Q Chen, K Kato, ME Pitulescu, RH Adams
    Elife, 2022-02-04;11(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  5. The onset of circulation triggers a metabolic switch required for endothelial to hematopoietic transition
    Authors: E Azzoni, V Frontera, G Anselmi, C Rode, C James, EM Deltcheva, AS Demian, J Brown, C Barone, A Patelli, JR Harman, M Nicholls, SJ Conway, E Morrissey, SEW Jacobsen, DB Sparrow, AL Harris, T Enver, MFTR de Bruijn
    Cell Reports, 2021-12-14;37(11):110103.
    Species: Mouse, Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. ABL001, a Bispecific Antibody Targeting VEGF and DLL4, with Chemotherapy, Synergistically Inhibits Tumor Progression in Xenograft Models
    Authors: DH Yeom, YS Lee, I Ryu, S Lee, B Sung, HB Lee, D Kim, JH Ahn, E Ha, YS Choi, SH Lee, WK You
    International Journal of Molecular Sciences, 2020-12-29;22(1):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Heritable modifiers of the tumor microenvironment influence nanoparticle uptake, distribution and response to photothermal therapy
    Authors: G Sharma, JM Jagtap, AK Parchur, VR Gogineni, S Ran, C Bergom, SB White, MJ Flister, A Joshi
    Theranostics, 2020-04-06;10(12):5368-5383.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  8. BMP9 signaling promotes the normalization of tumor blood vessels
    Authors: C Viallard, C Audiger, N Popovic, N Akla, K Lanthier, I Legault-Na, H Melichar, S Costantino, S Lesage, B Larrivée
    Oncogene, 2020-02-10;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Endothelial ZEB1 promotes angiogenesis-dependent bone formation and reverses osteoporosis
    Authors: R Fu, WC Lv, Y Xu, MY Gong, XJ Chen, N Jiang, Y Xu, QQ Yao, L Di, T Lu, LM Wang, R Mo, ZQ Wu
    Nat Commun, 2020-01-23;11(1):460.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. Lysophosphatidic acid-induced YAP/TAZ activation promotes developmental angiogenesis by repressing Notch ligand Dll4
    Authors: D Yasuda, D Kobayashi, N Akahoshi, T Ohto-Nakan, K Yoshioka, Y Takuwa, S Mizuno, S Takahashi, S Ishii
    J. Clin. Invest., 2019-07-23;130(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. Regulatory pathways governing murine coronary vessel formation are dysregulated in the injured adult heart
    Authors: S Payne, M Gunadasa-R, A Neal, AN Redpath, J Patel, KM Chouliaras, I Ratnayaka, N Smart, S De Val
    Nat Commun, 2019-07-22;10(1):3276.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  12. Fibrotic liver microenvironment promotes Dll4 and SDF-1-dependent T-cell lineage development
    Authors: Z Gong, B Shang, Y Chu, X Chen, Q Li, K Liu, Y Chen, Y Huang, Y Han, Q Shang, Z Zheng, L Song, Y Li, R Liu, C Xu, X Zhang, B Liu, L Wang, C Shao, Y Wang, Y Shi
    Cell Death Dis, 2019-06-05;10(6):440.
    Species: Human, Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  13. iSuRe-Cre is a genetic tool to reliably induce and report Cre-dependent genetic modifications
    Authors: M Fernández-, V Casquero-G, W Luo, F Francesca, S Ferreira R, S Del Olmo-C, R Benedito
    Nat Commun, 2019-05-22;10(1):2262.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-F
  14. Venous identity requires BMP signalling through ALK3
    Authors: A Neal, S Nornes, S Payne, MD Wallace, M Fritzsche, P Louphrasit, RN Wilkinson, KM Chouliaras, K Liu, K Plant, R Sholapurka, I Ratnayaka, W Herzog, G Bond, T Chico, G Bou-Ghario, S De Val
    Nat Commun, 2019-01-28;10(1):453.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  15. MPDZ promotes DLL4-induced Notch signaling during angiogenesis
    Authors: F Tetzlaff, MG Adam, A Feldner, I Moll, A Menuchin, J Rodriguez-, D Sprinzak, A Fischer
    Elife, 2018-04-05;7(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  16. The endothelial transcription factor ERG mediates Angiopoietin-1-dependent control of Notch signalling and vascular stability
    Authors: AV Shah, GM Birdsey, C Peghaire, ME Pitulescu, NP Dufton, Y Yang, I Weinberg, L Osuna Alma, L Payne, JC Mason, H Gerhardt, RH Adams, AM Randi
    Nat Commun, 2017-07-11;8(0):16002.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC-Fr, Western Blot
  17. O-GlcNAc on NOTCH1 EGF repeats regulates ligand-induced Notch signaling and vascular development in mammals
    Authors: Sawaguchi S, Varshney S, Ogawa M et al.
    eLife
  18. Lymphatic deletion of calcitonin receptor-like receptor exacerbates intestinal inflammation
    Authors: RB Davis, DO Kechele, ES Blakeney, JB Pawlak, KM Caron
    JCI Insight, 2017-03-23;2(6):e92465.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC
  19. Data showing proliferation and differentiation of intestinal epithelial cells under targeted depletion of Notch ligands in mouse intestine.
    Authors: Toru Nakata, Hiromichi Shimizu, Sayaka Nagata, Go Ito, Satoru Fujii, Kohei Suzuki, Ami Kawamoto, Fumiaki Ishibashi, Reiko Kuno, Sho Anzai, Tatsuro Murano, Tomohiro Mizutani, Shigeru Oshima, Kiichiro Tsuchiya, Tetsuya Nakamura, Katsuto Hozumi, Mamoru Watanabe, Ryuichi Okamoto
    Data in Brief, 2016-12-29;0(0):2352-3409.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  20. Blood flow controls bone vascular function and osteogenesis
    Nat Commun, 2016-12-06;7(0):13601.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  21. Notch Signaling Regulates the Homeostasis of Tissue-Restricted Innate-like T Cells
    Authors: Vijaykumar Chennupati
    J Immunol, 2016-06-20;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  22. Sox7, Sox17, and Sox18 Cooperatively Regulate Vascular Development in the Mouse Retina.
    Authors: Zhou Y, Williams J, Smallwood P, Nathans J
    PLoS ONE, 2015-12-02;10(12):e0143650.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  23. Interleukin-6 Stimulates Defective Angiogenesis.
    Authors: Gopinathan G, Milagre C, Pearce O, Reynolds L, Hodivala-Dilke K, Leinster D, Zhong H, Hollingsworth R, Thompson R, Whiteford J, Balkwill F
    Cancer Res, 2015-06-16;75(15):3098-107.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  24. Slit2 signaling through Robo1 and Robo2 is required for retinal neovascularization.
    Authors: Rama N, Dubrac A, Mathivet T, Ni Charthaigh R, Genet G, Cristofaro B, Pibouin-Fragner L, Ma L, Eichmann A, Chedotal A
    Nat Med, 2015-04-20;21(5):483-91.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  25. Selective neuronal lineages derived from Dll4-expressing progenitors/precursors in the retina and spinal cord.
    Authors: Zou M, Luo H, Xiang M
    Dev Dyn, 2014-09-16;244(1):86-97.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  26. Neuroligin 1 induces blood vessel maturation by cooperating with the alpha6 integrin.
    Authors: Samarelli A, Riccitelli E, Bizzozero L, Silveira T, Seano G, Pergolizzi M, Vitagliano G, Cascone I, Carpentier G, Bottos A, Primo L, Bussolino F, Arese M
    J Biol Chem, 2014-05-23;289(28):19466-76.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  27. Endothelial Notch activity promotes angiogenesis and osteogenesis in bone.
    Authors: Ramasamy S, Kusumbe A, Wang L, Adams R
    Nature, 2014-03-12;507(7492):376-80.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  28. Fbxw7 controls angiogenesis by regulating endothelial notch activity.
    Authors: Izumi N, Helker C, Ehling M, Behrens A, Herzog W, Adams RH
    PLoS ONE, 2012-07-27;7(7):e41116.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  29. Notch-dependent VEGFR3 upregulation allows angiogenesis without VEGF-VEGFR2 signalling.
    Authors: Benedito R, Rocha S, Woeste M, Zamykal M, Radtke F, Casanovas O, Duarte A, Pytowski B, Adams R
    Nature, 2012-03-18;484(7392):110-4.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  30. Delta1 expression, cell cycle exit, and commitment to a specific secretory fate coincide within a few hours in the mouse intestinal stem cell system.
    Authors: Stamataki D, Holder M, Hodgetts C
    PLoS ONE, 2011-09-07;6(9):e24484.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  31. Therapeutic efficacy of a DNA vaccine targeting the endothelial tip cell antigen delta-like ligand 4 in mammary carcinoma.
    Authors: Haller BK, Brave A, Wallgard E, Roswall P, Sunkari VG, Mattson U, Hallengard D, Catrina SB, Hellstrom M, Pietras K
    Oncogene, 2010-05-24;29(30):4276-86.
    Species: Human, Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC-Fr, Western Blot
  32. Blocking VEGFR-3 suppresses angiogenic sprouting and vascular network formation.
    Authors: Tammela T, Zarkada G, Wallgard E, Murtomaki A, Suchting S, Wirzenius M, Waltari M, Hellstrom M, Schomber T, Peltonen R, Freitas C, Duarte A, Isoniemi H, Laakkonen P, Christofori G, Yla-Herttuala S, Shibuya M, Pytowski B, Eichmann A, Betsholtz C, Alitalo K
    Nature, 2008-06-25;454(7204):656-60.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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