Mouse EBI3 Antibody Summary
Tyr19-Pro228 (EBI3) & Phe29-Ser234 (p28)
Accession # O35228 (EBI3) & Q8K3I6 (p28)
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Detection of Mouse IL‑27/IL‑35 EBI3 Subunit by Western Blot. Western blot shows lysates of CHO Chinese hamster ovary cell line either mock transfected or transfected with mouse EBI3. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse IL-27/IL-35 EBI3 Subunit Monoclonal Antibody (Catalog # MAB18341) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for IL-27/IL-35 EBI3 Subunit at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: EBI3
IL-27 is a heterodimeric cytokine comprised of the IL-12 p35-related protein, p28, and the IL-12 p40-related protein, EBI3 (Epstein-Barr virus-induced gene 3). IL-27 is expressed by monocytes, endothelial cells and dendritic cells. It binds TCCR/WSX-1 on naive CD4+ T cells and induces the expression of a functional IL-12 receptor, making these cells sensitive to IL-12-mediated Th1 cell development. Human EBI3 also associates with the p35 subunit of IL-12 to form IL-35 which is important for immunosuppressive Treg cell induction. EBI3 is 61% amino acid (aa) identical to mouse EBI3 and includes an 20 aa signal peptide anda 209 aa mature protein with two fibronectin type III domains.
Product Datasheets
Citations for Mouse EBI3 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
7
Citations: Showing 1 - 7
Filter your results:
Filter by:
-
B cell treatment promotes a neuroprotective microenvironment after traumatic brain injury through reciprocal immunomodulation with infiltrating peripheral myeloid cells
Authors: Dwyer, LJ;Maheshwari, S;Levy, E;Poznansky, MC;Whalen, MJ;S�rbulescu, RF;
Journal of neuroinflammation
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Interleukin-35 Prevents the Elevation of the M1/M2 Ratio of Macrophages in Experimental Type 1 Diabetes
Authors: Z Luo, C Soläng, R Larsson, K Singh
International Journal of Molecular Sciences, 2022-07-19;23(14):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Macrophage-mediated RON signaling supports breast cancer growth and progression through modulation of IL-35
Authors: SJ Ruiz-Torre, JR Bourn, NM Benight, BG Hunt, C Lester, SE Waltz
Oncogene, 2021-11-06;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Immunodepletion -
IL-30� (IL-27A): a familiar stranger in immunity, inflammation, and cancer
Authors: B Min, D Kim, MJ Feige
Experimental & Molecular Medicine, 2021-05-28;53(5):823-834.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Capture -
Oral Escherichia coli colonization factor antigen I fimbriae ameliorate arthritis via IL-35, not IL-27.
Authors: Kochetkova I, Thornburg T, Callis G, Holderness K, Maddaloni M, Pascual D
J Immunol, 2013-12-11;192(2):804-16.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
IL-35 stimulation of CD39+ regulatory T cells confers protection against collagen II-induced arthritis via the production of IL-10.
Authors: Kochetkova I, Golden S, Holderness K
J. Immunol., 2010-05-10;184(12):7144-53.
Species: Mouse
Sample Types: Recombinant Protein
Applications: Western Blot -
Tolerogenic signals delivered by dendritic cells to T cells through a galectin-1-driven immunoregulatory circuit involving interleukin 27 and interleukin 10.
Authors: Ilarregui JM, Croci DO, Bianco GA, Toscano MA, Salatino M, Vermeulen ME, Geffner JR, Rabinovich GA
Nat. Immunol., 2009-08-09;10(9):981-91.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Mouse EBI3 Antibody
Average Rating: 4 (Based on 1 Review)
Have you used Mouse EBI3 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
