Mouse
EpCAM/TROP-1 PE-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: EpCAM/TROP1

Epithelial Cellular Adhesion Molecule (EpCAM), also known as ECD326 and TROP-1 is a 36-41 kDa member of the TROP family of meolcules (1, 2). It is a type I transmembrane glycoprotein that is found on embryonic stem cells and adult non-squamous epithelium such as (respiratory) psuedostratified, (mammary) cuboid, and (colon) simple columnar epithelium. In mouse, the molecule has also been reported on T cells, Langerhans cells and thymic epithelium. Mature mouse EpCAM is 292 amino acids (aa) in length. It possesses a 243 aa extracellular region that contains one thyroglobulin type I domain. Such domains are associated with cathepsin binding and inhibition (2). On the cell surface, EpCAM is reported to participate in TEM (Tetraspanin-enriched microdomain) formation in concert with CD44v6, CD9, ADAM10, Claudin-7 and TS8. It is also noted to form homodimers in-cis and homotetramers in-trans. Although this would suggest the EpCAM acts as an adhesion molecule, in effect it does not, instead acting as a disruptor of the E-Cadherin:cytoskeleton interaction, and thus promoting cell migration (1, 2). The extracellular domain (ECD) of EpCAM undergoes multiple cleavages, principally between Ser200 and Ala210 (3). Cleavage at the Arg80-Arg81 site does not generate a soluble fragment, but rather a disulfide-linked "heterodimer" (3). Cleavage after Gly264 in the juxtamembrane region is followed by secretase cleavage of the transmembrane fragment, generating an ICD that translocates to the nucleus (4). The ECD of mouse EpCAM shares 81% and 90% aa sequence identity with human and rat EpCAM, respectively.

Product Datasheets

Related Research Areas

• Adhesion Molecules in Angiogenesis
• Cancer Biomarkers
• Cancer Stem Cell Markers
• Conventional/Classical Dendritic Cells
• Embryonic and Induced Pluripotent Stem Cell Markers
• Epithelial Cell Markers and Intracellular Molecules
• Immunoglobulin Superfamily CAMs
• Langerhans and Dermal Dendritic Cells
• Surface Ectodermal Cells
• Trophoblast Stem Cell Markers