Mouse EphA2 Antibody Summary
Ala22-Ala535
Accession # AAA82113
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: EphA2
EphA2, also known as Eck, Myk2, and Sek2 (1), is a member of the Eph receptor family which binds members of the ephrin ligand family. There are two classes of receptors, designated A and B. Both the A and B class receptors have an extracellular region consisting of a globular domain, a cysteine-rich domain, and two fibronectin type III domains. This is followed by the transmembrane region and cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxy tail which contains one conserved tyrosine residue. Activation of kinase activity occurs after ligand recognition and binding. EphA2 has been shown to bind ephrin-A3, ephrin-A1, ephrin-A5, ephrin-A4, and ephrin-A2 (2, 3). The extracellular domains of mouse and human EphA2 share greater than 92% amino acid identity. Only membrane-bound or Fc‑clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/ephrin families also appear to play a role in angiogenesis (3).
- Eph Nomenclature Committee [letter] (1997) Cell 90:403.
- Flanagan, J.G. and P. Vanderhaegen (1998) Annu. Rev. Neurosci. 21:309.
- Pasquale, E.B. (1997) Curr. Opin. Cell. Biol. 9:608.
Product Datasheets
Citations for Mouse EphA2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Genotype, Age, Genetic Background, and Sex Influence Epha2-Related Cataract Development in Mice
Authors: A Dave, JE Craig, M Alamein, K Skrzypiec, J Beltz, A Pfaff, KP Burdon, N Ercal, RU de Iongh, S Sharma
Investigative Ophthalmology & Visual Science, 2021-09-02;62(12):3.
Species: Mouse
Sample Types: WholeTissue
Applications: IHC -
Brusatol-Mediated Inhibition of c-Myc Increases HIF-1? Degradation and Causes Cell Death in Colorectal Cancer under Hypoxia
Authors: ET Oh, CW Kim, HG Kim, JS Lee, HJ Park
Theranostics, 2017-08-11;7(14):3415-3431.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
EphA2 and ephrin-A5 are not a receptor-ligand pair in the ocular lens
Authors: C Cheng, VM Fowler, X Gong
Exp. Eye Res., 2017-06-23;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Ginsenoside Rg3 inhibition of vasculogenic mimicry in pancreatic cancer through downregulation of VEcadherin/EphA2/MMP9/MMP2 expression.
Authors: Guo J, Zheng Q, Chen H, Chen L, Xu J, Chen M, Lu D, Wang Z, Tong H, Lin S
Int J Oncol, 2014-06-16;45(3):1065-72.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Capillary defects and exaggerated inflammatory response in the airways of EphA2-deficient mice.
Authors: Okazaki T, Ni A, Baluk P, Ayeni OA, Kearley J, Coyle AJ, Humbles A, McDonald DM
Am. J. Pathol., 2009-05-14;174(6):2388-99.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Bidirectional signaling through ephrinA2-EphA2 enhances osteoclastogenesis and suppresses osteoblastogenesis.
Authors: Irie N, Takada Y, Watanabe Y, Matsuzaki Y, Naruse C, Asano M, Iwakura Y, Suda T, Matsuo K
J. Biol. Chem., 2009-03-19;284(21):14637-44.
Species: Mouse
Sample Types: Cell Lysates, Whole Tissue
Applications: IHC, Western Blot -
Disruption of EphA2 receptor tyrosine kinase leads to increased susceptibility to carcinogenesis in mouse skin.
Authors: Guo H, Miao H, Gerber L, Singh J, Denning MF, Gilliam AC, Wang B
Cancer Res., 2006-07-15;66(14):7050-8.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr
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