Mouse Fas Ligand/TNFSF6 Antibody

Name: Mouse Fas Ligand/TNFSF6 Antibody
Brand: R&D Systems
SKU: MAB5262
Rating: 5 (1 reviews)

Catalog #: MAB5262
5 Citations 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5262
MAB5262-SP

Cytotoxicity Induced by Fas Ligand/TNFSF6 and Neutralization by Mouse Fas Ligand/TNFSF6 Antibody.

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Citations (5)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Fas Ligand/TNFSF6 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Fas Ligand/TNFSF6 in ELISAs and Western blots. In Western blots, approximately 2% cross-reactivity with recombinant mouse (rm) TNF-alpha is observed and no cross-reactivity with recombinant human (rh) APRIL, rhFas Ligand, rhGITR Ligand, rhLIGHT, rhTRAIL, rmTRANCE, and rhVEGI is observed.

Source

Monoclonal Rat IgG₁ Clone # 101626

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Fas Ligand/TNFSF6
Pro132-Leu279
Accession # P41047

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse Fas Ligand/TNFSF6 (Catalog # 526-SA)

Mouse Fas Ligand/TNFSF6 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Mouse Fas Ligand/TNFSF6 Biotinylated Antibody (Catalog # BAF526)

Standard: Recombinant Mouse Fas Ligand/TNFSF6 Protein (Catalog # 526-SA)

Neutralization

Measured by its ability to neutralize Fas Ligand/TNFSF6-induced cytotoxicity in the Jurkat human acute T cell leukemia cell line. The Neutralization Dose (ND₅₀) is typically 5-20 µg/mL in the presence of 5 µg/mL Recombinant Mouse Fas Ligand/TNFSF6 and 10 µg/mL of a cross-linking antibody, Mouse polyHistidine Monoclonal Antibody.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Cytotoxicity Induced by Fas Ligand/TNFSF6 and Neutralization by Mouse Fas Ligand/TNFSF6 Antibody. In the presence of a cross-linking antibody, Mouse polyHisti-dine Monoclonal Antibody (10 µg/mL, Catalog # MAB050), Recom-binant Mouse Fas Ligand/ TNFSF6 (Catalog # 526-SA) induces cytotoxicity in the Jurkat human acute T cell leukemia cell line in a dose-dependent manner (orange line). Under these conditions, cytotoxicity elicited by Recombinant Mouse Fas Ligand/ TNFSF6 (5 µg/mL) is neutral-ized (green line) by increasing concentrations of Rat Anti-Mouse Fas Ligand/TNFSF6 Monoclonal Antibody (Catalog # MAB5262). The ND₅₀ is typically 5-20 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fas Ligand/TNFSF6

Fas Ligand (FasL) is a 40 kDa type II membrane protein belonging to the TNF family. In the new TNF super family nomenclature, FasL is referred to as TNFSF6. The specific receptor for FasL is Fas (CD95, Apo-1), a 45 kDa type I transmembrane protein that is a member of the TNF receptor family. FasL is predominantly expressed on activated T cells and NK cells, while Fas is expressed on various types of cells. The Fas/FasL system plays a crucial role in modulating immune response by inducing cell apoptosis to maintain homeostasis, self-tolerance of lymphocytes, and immune privilege. FasL was reported to be a potent chemoattractant for neutrophils, suggesting a novel proinflammatory function of this molecule. Like other members of the TNF family, the membrane-bound FasL can be cleaved by metalloproteinase to generate the soluble Fas ligand (sFasL) which is mainly a non-covalently linked homotrimer. It has been shown that the membrane-bound TNF‑ alpha and FasL are primary activators of their receptors. In contrast to soluble TNF‑ alpha which has potent cytotoxicity, sFasL is much less cytotoxic. Studies have shown that sFasL may competitively inhibit the killing effect of membrane FasL indicating that the cleaving of membrane FasL might be a mechanism to down‑regulate their activities.

References

Suda, T. et al. (1993) Cell 75:1169.
Kägi, D. et al. (1994) Science 265:528.
Schneider, P. et al. (1998) J. Exp. Med. 187:1205.
Seino, K. et al. (1998) J. Immunol. 161:4484.

Entrez Gene IDs

356 (Human); 14103 (Mouse); 25385 (Rat)

Alternate Names

apoptosis (APO-1) antigen ligand 1; Apoptosis antigen ligand; APT1LG1CD95L; APTL; CD178 antigen; CD178; CD95L; CD95-L; Fas antigen ligand; Fas ligand (TNF superfamily, member 6); Fas Ligand; FASLCD95 ligand; FASLG; TNFSF6; TNFSF6FasL; tumor necrosis factor (ligand) superfamily, member 6; tumor necrosis factor ligand superfamily member 6

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse Fas Ligand/TNFSF6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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FasL Modulates Expression of Mmp2 in Osteoblasts
Authors: E Svandova, B Vesela, H Lesot, J Sadoine, A Poliard, E Matalova
Front Physiol, 2018-09-19;9(0):1314.
Species: Mouse
Sample Types: Whole Cells
Applications: Inhibition
Glycolysis promotes caspase-3 activation in lipid rafts in T cells
Authors: MA Secinaro, KA Fortner, O Dienz, A Logan, MP Murphy, V Anathy, JE Boyson, RC Budd
Cell Death Dis, 2018-01-19;9(2):62.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Response patterns of cytokines/chemokines in two murine strains after irradiation.
Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
Cytokine, 2012-01-25;58(2):169-77.
Species: Mouse
Sample Types: Plasma
Applications: Luminex Development
Antigen-specific CD25- Foxp3- IFN-gamma(high) CD4+ T cells restrain the development of experimental allergic encephalomyelitis by suppressing Th17.
Authors: Wildbaum G, Zohar Y, Karin N
Am. J. Pathol., 2010-04-09;176(6):2764-75.
Species: Mouse
Sample Types: In Vivo, Whole Cells
Applications: Neutralization
Estrogen protects bone by inducing Fas ligand in osteoblasts to regulate osteoclast survival.
Authors: Krum SA, Miranda-Carboni GA, Hauschka PV, Carroll JS, Lane TF, Freedman LP, Brown M
EMBO J., 2008-01-24;27(3):535-45.
Species: Mouse
Sample Types: Whole Cells, Whole Tissue
Applications: IHC-P, Neutralization