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Mouse Fc gamma RIII (CD16) APC-conjugated Antibody

Catalog #: FAB19601A
6 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB19601A-025
FAB19601A-100
Detection of Fc gamma RIII (CD16) in RAW 264.7 Mouse Cell Line by Flow Cytometry.
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Citations (6)
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Mouse Fc gamma RIII (CD16) APC-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Fc gamma RIIIA/B (CD16) in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Fcrl3/CD16-2, rmFc gamma RIA or rhFc gamma RIIA is observed.
Source
Monoclonal Rat IgG2A Clone # 275003
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Fc gamma  RIII
Ala31-Thr215
Accession # Q5D5J5
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Fc gamma RIII (CD16) antibody in RAW 264.7 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of Fc gamma RIII (CD16) in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse Fc gamma RIII (CD16) APC-conjugated Monoclonal Antibody (Catalog # FAB19601A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Fc gamma RIII (CD16)

Receptors for the Fc region of IgG (Fc gamma Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, ADCC (antibody-dependent cellular toxicity), cytokine release, and B cell proliferation (1-3). The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma  RI (also known as CD64), Fc gamma  RII (CD32), and Fc gamma  RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors
(~10-8-10-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10-6-10-7 M) only recognizing IgG aggregates surrounding multivalent antigens (1, 4). Fc gamma Rs that deliver an activating signal either have an intrinsic immunoreceptor tyrosine-based activation motif (ITAM) within their cytoplasmic domains or associate with one of the ITAM-bearing adapter subunits, Fc R gamma or zeta (3, 5). The only inhibitory member in human and mouse, Fc gamma  RIIb, has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM). The coordinated functioning of activating and inhibitory receptors is necessary for successful initiation, amplification, and termination of immune responses (5). Mouse CD16 is encoded by a single gene. The protein product is a type I transmembrane protein having two extracellular Ig-like domains. It is expressed on a variety of myeloid and lymphoid cells (4) and associates with Fc R gamma to deliver an activating signal upon ligand binding (5). Mouse CD32 is closely related to mouse CD16 throughout its extracellular domain (95% amino acid sequence identity), but has a divergent cytoplasmic domain and functions as an inhibitory receptor. Together these proteins constitute an activating/inhibiting receptor pair to regulate immune responses (5).

References
  1. van de Winkel, J. and P. Capes (1993) Immunol. Today 14:215. 
  2. Raghaven, M. and P. Bjorkman (1996) Annu. Rev. Cell Dev. Biol. 12:181. 
  3. Ravetch, J. and S. Bolland (2001) Annu. Rev. Immunol. 19:275.
  4. Takai, T. (2002) Nature Rev. Immunol. 2:580.
  5. Ravetch, J. and L. Lanier (2000) Science 290:84.
Long Name
Fc gamma Receptor III
Entrez Gene IDs
14131 (Mouse); 102140945 (Cynomolgus Monkey)
Alternate Names
CD16; CD16A; Fc fragment of IgG receptor IIIa; Fc gamma RIII; FCG3; FCGR3; FcgRIII; FCR-10; FCRIII; FCRIIIA; IGFR3; IMD20

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Citations for Mouse Fc gamma RIII (CD16) APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Fc?R-dependent apoptosis regulates tissue persistence of mucosal and connective tissue mast cells
    Authors: Wu, T;Yan, S;Yeh, YW;Fang, Y;Xiang, Z;
    European journal of immunology
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. In vivo study of the immune response to bioengineered spider silk spheres
    Authors: T Deptuch, K Penderecka, M Kaczmarek, S Molenda, H Dams-Kozlo
    Scientific Reports, 2022-08-05;12(1):13480.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Tumor-Released Products Promote Bone Marrow-Derived Macrophage Survival and Proliferation
    Authors: JM Motta, VM Rumjanek, A Mantovani, M Locati
    Biomedicines, 2021-10-04;9(10):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. MafB enhances the phagocytic activity of RAW264.7 macrophages by promoting Fcgr3 expression
    Authors: Isao Kubota
    Biochem. Biophys. Res. Commun., 2016-11-12;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Investigation of the Immunomodulatory effect of Berberis vulgaris on core-pulsed dendritic cell vaccine
    BMC Complement Altern Med, 2016-08-30;16(1):325.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Fcgamma receptors inhibit mouse and human basophil activation.
    Authors: Cassard L, Jonsson F, Arnaud S, Daeron M
    J Immunol, 2012-08-20;189(6):2995-3006.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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