Mouse Fetuin A/AHSG Biotinylated Antibody

Catalog #: BAM15631 Datasheet / COA / SDS

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BAM15631

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Fetuin A/AHSG Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Fetuin A/AHSG in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant human (rh) Fetuin A, rhTGF-beta 1, recombinant mouse (rm) BMP-4, rmMMP-2, recombinant rat MMP-2, or rmMMP-9 is observed.

Source

Monoclonal Rat IgG_2A Clone # 207432

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Fetuin A/AHSG
Ala19-Ile345
Accession # P29699

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Mouse Fetuin A/AHSG Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.5-2.0 µg/mL

Use in combination with:

Capture Reagent: Mouse Fetuin A/AHSG Antibody (Catalog # MAB15632)

Standard: Recombinant Mouse Fetuin A/AHSG Protein, CF (Catalog # 1563-PI)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fetuin A/AHSG

Mouse Fetuin A, also known as alpha ₂-Heremans-Schmid glycoprotein, is encoded by the AHSG gene. It has been also called "countertrypsin" because of its ability to inhibit trypsin (1). It is a major plasma protein and a member of the cystatin superfamily of protease inhibitors (2, 3). It is expressed by hepatocytes, the principal cell source, and by monocyte/macrophages (4). The major form of plasma Fetuin A corresponds to two disulfide bond-linked chains derived from the single chain (5). Fetuin-A has a number of functions. It is a negative acute-phase protein with normal circulating levels in adults (300‑600 μg/mL), which fall significantly (30‑50%) during injury and infection (5). It enhances entry of cationic inhibitors into macrophages (6). It inhibits both insulin receptor autophosphorylation and undesirable calcification (7, 8).

References