Mouse Fibroblast Activation Protein alpha /FAP Alexa Fluor® 405-conjugated Antibody

Catalog #: FAB9727V Datasheet / COA / SDS

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FAB9727V-100UG

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Mouse Fibroblast Activation Protein alpha /FAP Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Fibroblast Activation Protein alpha /FAP in direct ELISAs.

Source

Monoclonal Rat IgG1 Clone # 983802

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25-1 µg/10⁶ cells

C2C12 mouse myoblast cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitution default not known

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Fibroblast Activation Protein alpha/FAP

FAP (also known as seprase) is a 95 kDa Type II transmembrane serine protease that is structurally related to dipeptidyl peptidase IV (DPPIV/CD26) (1, 2). Within the extracellular domain, mouse FAP shares 90% and 97% amino acid (aa) sequence identity with human and rat FAP, respectively (3, 4). Alternative splicing of mouse FAP generates isoforms with a 33 aa or 5 aa deletion in the extracellular juxtamembrane region (3). FAP is expressed on reactive stromal fibroblasts in tumor tissue and wound healing and on synoviocytes in rheumatoid arthritis (1, 5-7). It exhibits dipeptidyl peptidase activity with substrate specificity similar to DPPIV, which is specific for N-terminal Xaa-Pro sequences (5, 8). FAP is also an endopeptidase that can degrade Gelatin, Collagens I and IV, Fibronectin, and Laminin (1, 5, 8) as well as several peptide hormones (e.g. Neuropeptide Y, Brain Natriuretic Peptide, Substance P, Peptide YY, and Incretins) (9). The enzymatic activity is dependent on FAP association with DPPIV on the cell surface (5, 8, 10, 11). The matrix-dedgrading activity of FAP contributes to tumor cell migration and invasion (10-13). In addition, FAP can enhance tumor cell growth by limiting the development of anti-tumor immunity (14).

References

Zi, F. et al. (2015) Mol. Med. Rep. 11:3203.
Pineiro-Sanchez, M.L. et al. (1997) J. Biol. Chem. 272:7595.
Niedermeyer, J. et al. (1997) Int. J. Cancer 71:383.
Scanlan, M.J. et al. (1994) Proc. Natl. Acad. Sci. USA 91:5657.
Park, J.E. et al. (1999) J. Biol. Chem. 274:36505.
Rettig, W.J. et al. (1988) Proc. Natl. Acad. Sci. USA 85:3110.
Bauer, S. et al. (2006) Arthritis Res. 8:R171.
Aertgeerts, K. et al. (2005) J. Biol. Chem. 280:19441.
Keane, F.M. et al. (2011) FEBS J. 278:1316.
Ghersi, G. et al. (2006) Cancer Res. 66:4652.
Ghersi, G. et al. (2002) J. Biol. Chem. 277:29231.
Cheng, J.D. et al. (2005) Mol. Cancer Ther. 4:351.
Cheng, J.D. et al. (2002) Cancer Res. 62:4767.
Kraman, M. et al. (2010) Science 330:827.

Entrez Gene IDs

2191 (Human); 14089 (Mouse); 102134935 (Cynomolgus Monkey)

Alternate Names

170 kDa melanoma membrane-bound gelatinase; DKFZp686G13158; DPPIV; EC 3.4.21.-; FAP; FAPA; Fibroblast Activation Protein alpha; fibroblast activation protein, alpha; Integral membrane serine protease; Seprase; vibronectin

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Product Specific Notices

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.