Mouse Fibroblast Activation Protein alpha /FAP Antibody

Catalog #: MAB9727
4 Citations Datasheet / COA / SDS

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MAB9727-100
MAB9727-SP

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Detection of Fibroblast Activation Protein alpha/FAP in C2C12 Mouse Cell Line by Flow Cytometry.

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All Fibroblast Activation Protein alpha/FAP Antibodies

Product Details

Citations (4)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Fibroblast Activation Protein alpha /FAP Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Fibroblast Activation Protein alpha /FAP in direct ELISAs.

Source

Monoclonal Rat IgG₁ Clone # 983802

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Fibroblast Activation Protein alpha /FAP
Leu26-Asp761
Accession # P97321

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA capture antibody when paired with Rat Anti-Mouse Fibroblast Activation Protein alpha /FAP Monoclonal Antibody (Catalog # MAB97271).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Flow Cytometry

0.25 µg/10⁶ cells

See below

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Fibroblast Activation Protein alpha/FAP in C2C12 Mouse Cell Line by Flow Cytometry. C2C12 mouse myoblast cell line was stained with Rat Anti-Mouse Fibroblast Activation Protein alpha/FAP Monoclonal Antibody (Catalog # MAB9727, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry

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Fibroblast Activation Protein alpha /FAP in C2C12 Mouse Cell Line. Fibroblast Activation Protein a/FAP was detected in immersion fixed C2C12 mouse myoblast cell line using Rat Anti-Mouse Fibroblast Activation Protein a/FAP Monoclonal Antibody (Catalog # MAB9727) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fibroblast Activation Protein alpha/FAP

FAP (also known as seprase) is a 95 kDa Type II transmembrane serine protease that is structurally related to dipeptidyl peptidase IV (DPPIV/CD26) (1, 2). Within the extracellular domain, mouse FAP shares 90% and 97% amino acid (aa) sequence identity with human and rat FAP, respectively (3, 4). Alternative splicing of mouse FAP generates isoforms with a 33 aa or 5 aa deletion in the extracellular juxtamembrane region (3). FAP is expressed on reactive stromal fibroblasts in tumor tissue and wound healing and on synoviocytes in rheumatoid arthritis (1, 5-7). It exhibits dipeptidyl peptidase activity with substrate specificity similar to DPPIV, which is specific for N-terminal Xaa-Pro sequences (5, 8). FAP is also an endopeptidase that can degrade Gelatin, Collagens I and IV, Fibronectin, and Laminin (1, 5, 8) as well as several peptide hormones (e.g. Neuropeptide Y, Brain Natriuretic Peptide, Substance P, Peptide YY, and Incretins) (9). The enzymatic activity is dependent on FAP association with DPPIV on the cell surface (5, 8, 10, 11). The matrix-dedgrading activity of FAP contributes to tumor cell migration and invasion (10-13). In addition, FAP can enhance tumor cell growth by limiting the development of anti-tumor immunity (14).

References

Zi, F. et al. (2015) Mol. Med. Rep. 11:3203.
Pineiro-Sanchez, M.L. et al. (1997) J. Biol. Chem. 272:7595.
Niedermeyer, J. et al. (1997) Int. J. Cancer 71:383.
Scanlan, M.J. et al. (1994) Proc. Natl. Acad. Sci. USA 91:5657.
Park, J.E. et al. (1999) J. Biol. Chem. 274:36505.
Rettig, W.J. et al. (1988) Proc. Natl. Acad. Sci. USA 85:3110.
Bauer, S. et al. (2006) Arthritis Res. 8:R171.
Aertgeerts, K. et al. (2005) J. Biol. Chem. 280:19441.
Keane, F.M. et al. (2011) FEBS J. 278:1316.
Ghersi, G. et al. (2006) Cancer Res. 66:4652.
Ghersi, G. et al. (2002) J. Biol. Chem. 277:29231.
Cheng, J.D. et al. (2005) Mol. Cancer Ther. 4:351.
Cheng, J.D. et al. (2002) Cancer Res. 62:4767.
Kraman, M. et al. (2010) Science 330:827.

Entrez Gene IDs

2191 (Human); 14089 (Mouse); 102134935 (Cynomolgus Monkey)

Alternate Names

170 kDa melanoma membrane-bound gelatinase; DKFZp686G13158; DPPIV; EC 3.4.21.-; FAP; FAPA; Fibroblast Activation Protein alpha; fibroblast activation protein, alpha; Integral membrane serine protease; Seprase; vibronectin

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Citations for Mouse Fibroblast Activation Protein alpha /FAP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Stromal depletion by TALEN-edited universal hypoimmunogenic FAP-CAR T cells enables infiltration and anti-tumor cytotoxicity of tumor antigen-targeted CAR-T immunotherapy
Authors: Shipra Das, Julien Valton, Philippe Duchateau, Laurent Poirot
Frontiers in Immunology
Tumor Suppression by Anti-Fibroblast Activation Protein Near-Infrared Photoimmunotherapy Targeting Cancer-Associated Fibroblasts
Authors: Glabman, RA;Olkowski, CP;Minor, HA;Bassel, LL;Kedei, N;Choyke, PL;Sato, N;
Cancers
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Pancreatic tumor eradication via selective Pin1 inhibition in cancer-associated fibroblasts and T lymphocytes engagement
Authors: J Liu, Y Wang, C Mu, M Li, K Li, S Li, W Wu, L Du, X Zhang, C Li, W Peng, J Shen, Y Liu, D Yang, K Zhang, Q Ning, X Fu, Y Zeng, Y Ni, Z Zhou, Y Liu, Y Hu, X Zheng, T Wen, Z Li, Y Liu
Nature Communications, 2022-07-25;13(1):4308.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
In utero estrogenic endocrine disruption alters the stroma to increase extracellular matrix density and mammary gland stiffness
Authors: C Wormsbaech, AR Hindman, A Avendano, M Cortes-Med, CE Jones, A Bushman, L Onua, CE Kovalchin, AR Murphy, HL Helber, A Shapiro, K Voytovitch, X Kuang, R Aguilar-Va, JL Leight, JW Song, CJ Burd
Breast Cancer Res., 2020-05-05;22(1):41.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry