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Mouse Frizzled-1 Antibody

Catalog #: AF1120
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1120
AF1120-SP
Frizzled‑1 in Embryonic Mouse Intestine.
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Product Details
Citations (5)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Frizzled-1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Frizzled-1 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant mouse (rm) Frizzled-2, rmFrizzled-4, rmFrizzled-7, and rmFrizzled-8 is observed, and less than 1% cross-reactivity with rmFrizzled-3 and rmFrizzled-6 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Frizzled‑1
Gln72-His248 (Met122Ile)
Accession # O70421
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Frizzled‑1 Fc Chimera (Catalog # 1120-FZ)
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Frizzled‑1 antibody in Embryonic Mouse Intestine by Immunohistochemistry (IHC-Fr). View Larger

Frizzled‑1 in Embryonic Mouse Intestine. Frizzled-1 was detected in immersion fixed frozen sections of embryonic mouse intestine (13 d.p.c.) using 15 µg/mL Goat Anti-Mouse Frizzled-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1120) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of epithelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Frizzled-1

The Wnt genes encode a large family of glycoproteins that are essential in development and tissue maintenance (1). Members of the Frizzled family of proteins serve as receptors for the Wnt signaling pathway (2). Ten mouse and human Frizzled genes have been identified to date. The predicted structure of Frizzled proteins is similar among all family members, containing a divergent N-terminal signal peptide, a highly conserved extracellular cysteine-rich domain (CRD), a variable-length linker region, a seven-pass transmembrane region, and a variable-length C-terminal cytoplasmic domain. The CRD, which comprises 642 amino acid residues and shares 95% identity with the human orthologue in the CRD. Frizzled-1 mRNA has been detected in relatively large amounts in adult heart, placenta, lung, kidney, pancreas, prostate, and ovary, and in fetal lung and kidney (3).

Several Frizzled-dependent signaling pathways exist (2). Their activation depends on the Wnt ligand and the cell context. Members of the low density lipoprotein receptor-related protein (LRP) are co-receptors for the Wnt ligands. LRP5/6 serve as co-receptors in the Wnt/Frizzled canonical pathway that alters gene expression via the stabilization of beta -catenin (4, 5). LRP1 may down regulate Wnt-3a/Frizzled-1 signaling in the canonical pathway by sequestering Frizzled-1 (6). Frizzled-1 is one of the purported Wnt-10b receptors whose signaling inhibits adipogenesis in preadipocytes (7). Frizzled-1 may be part of a feedback mechanism to modulate the effects of BMP-2 in mesenchymal cells since upregulation of its expression by BMP-2 counteracts the effects of BMP-2 and Wnt-3a in inducing the expression of the osteoblast differentiation marker, alkaline phosphatase (8).

References
  1. Wodarz, A. and R. Nusse (1998) Annu. Rev. Cell Dev. Biol. 14:59. 
  2. Hsieh, J-C. (2004) Front. Biosci., 9:1333. 
  3. Xu, L. et al. (2001) Matrix Biol. 2:147. 
  4. Tamai, K. et al. (2000) Nature 407:530. 
  5. Pinson, K. et al. (2000) Nature 407:535.
  6. Zielberberg, A. et al. (2004) J. Biol. Chem. 279:17535.
  7. Bennet, C. et al. (2002) J. Biol. Chem. 277:30998.
  8. Roman-Roman, S. et al. (2004) J. Biol. Chem. 279:5725.
Entrez Gene IDs
8321 (Human); 14362 (Mouse)
Alternate Names
DKFZp564G072; FLJ95923; frizzled family receptor 1; frizzled homolog 1; frizzled, Drosophila, homolog of, 1; Frizzled1; Frizzled-1; fz-1; FZD1; fzE1; hFz1; seven transmembrane spanning receptor; Wnt receptor

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Citations for Mouse Frizzled-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. A Multilevel Screening Strategy Defines a Molecular Fingerprint of Proregenerative Olfactory Ensheathing Cells and Identifies SCARB2, a Protein That Improves Regenerative Sprouting of Injured Sensory Spinal Axons.
    Authors: Roet KC, Franssen EH, de Bree FM et al.
    J Neurosci
  2. Comparative Analysis of the Development of Acquired Radioresistance in Canine and Human Mammary Cancer Cell Lines
    Authors: Mark Gray, Arran K. Turnbull, James Meehan, Carlos Martínez-Pérez, Charlene Kay, Lisa Y. Pang et al.
    Frontiers in Veterinary Science
  3. Wnt-5a/Frizzled9 receptor signaling through the G?o/G?? complex regulates dendritic spine formation
    J Biol Chem, 2016-07-11;0(0):.
    Applications: Bioassay
  4. Frizzled-1 is involved in the neuroprotective effect of Wnt3a against Abeta oligomers.
    Authors: Chacon MA, Varela-Nallar L, Inestrosa NC
    J. Cell. Physiol., 2008-10-01;217(1):215-27.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  5. Fbxw7 acts as an E3 ubiquitin ligase that targets c-Myb for nemo-like kinase (NLK)-induced degradation.
    Authors: Kanei-Ishii C, Nomura T, Takagi T, Watanabe N, Nakayama KI, Ishii S
    J. Biol. Chem., 2008-09-02;283(45):30540-8.
    Species: Rat
    Sample Types: Recombinant Protein
    Applications: Western Blot

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