Mouse GPVI Antibody

Catalog #: MAB6758 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6758
MAB6758-SP

Detection of GPVI in Mouse platelets by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse GPVI Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse GPVI in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human GPVI is observed.

Source

Monoclonal Rat IgG₁ Clone # 784808

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse GPVI
Gly24-Lys265
Accession # P0C191

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of GPVI in Mouse platelets by Flow Cytometry. Mouse platelets were stained with Rat Anti-Mouse GPVI Monoclonal Antibody (Catalog # MAB6758, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).

Immunocytochemistry

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GPVI in Mouse Platelets. GPVI was detected in immersion fixed adult mouse platelets using Rat Anti-Mouse GPVI Monoclonal Antibody (Catalog # MAB6758) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI. Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GPVI

GPVI (Platelet Glycoprotein VI; also glycoprotein 5) is a member of the Ig superfamily. It is found on platelets and megakaryocytes, and serves as the main collagen receptor on platelets. Following exposure to subendothelial connective tissue, GPVI binds to a Gly-Pro-(hydroxy)Pro motif on collagen and generates a noncovalent membrane signaling complex with FcR gamma -chain. This interaction is stabilized by Integrin alpha 2 beta 1, followed by activation of PLC gamma 2 with clot initiation. Mature mouse GPVI is a 292 amino acid (aa) type I transmembrane protein. It possesses a 244 aa extracellular region (aa 22-265) that contains two C2-type Ig-like domains (aa 27-197)and two potential glycosylation sites, plus a 37 aa cytoplasmic tail (aa 287-313). There is one potential splice form that shows a deletion of aa 224-240. Over aa 24-265, mouse GPVI shares 70% and 86% aa identity with human and rat GPVI, respectively.

Long Name

Glycoprotein VI [Platelet]

Entrez Gene IDs

51206 (Human); 243816 (Mouse)

Alternate Names

Glycoprotein 6; glycoprotein VI (platelet); GP6; GPIV; GPVI; GPVIplatelet collagen receptor; MGC138168; platelet glycoprotein VI