Mouse GPVI PE-conjugated Antibody

Catalog #: FAB6758P
1 Citation Datasheet / COA / SDS

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FAB6758P

Detection of GPVI in Mouse Platelets by Flow Cytometry.

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Mouse GPVI PE-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse GPVI in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human GPVI is observed.

Source

Monoclonal Rat IgG₁ Clone # 784808

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse GPVI
Gly24-Lys265
Accession # P0C191

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of GPVI in Mouse Platelets by Flow Cytometry. Mouse platelets were stained with Rat Anti-Mouse GPVI PE-conjugated Monoclonal Antibody (Catalog # FAB6758P, filled histogram) or isotype control antibody (Catalog # IC005P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: GPVI

GPVI (Platelet Glycoprotein VI), also known as Glycoprotein 5, is a member of the Ig superfamily. It is found on platelets and megakaryocytes, and serves as the main collagen receptor on platelets. Following exposure to subendothelial connective tissue, GPVI binds to a Gly-Pro-(hydroxy)Pro motif on collagen and generates a noncovalent membrane signaling complex with FcR gamma -chain. This interaction is stabilized by Integrin alpha 2 beta 1, followed by activation of PLC gamma 2 with clot initiation. Mature mouse GPVI is a 292 amino acid (aa) type I transmembrane protein. It possesses a 244 aa extracellular region (aa 22-265) that contains two C2-type Ig-like domains (aa 27-197)and two potential glycosylation sites, plus a 37 aa cytoplasmic tail (aa 287-313). There is one potential splice form that shows a deletion of aa 224-240. Over aa 24-265, mouse GPVI shares 70% and 86% aa identity with human and rat GPVI, respectively.

Long Name

Glycoprotein VI [Platelet]

Entrez Gene IDs

51206 (Human); 243816 (Mouse)

Alternate Names

Glycoprotein 6; glycoprotein VI (platelet); GP6; GPIV; GPVI; GPVIplatelet collagen receptor; MGC138168; platelet glycoprotein VI

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Citation for Mouse GPVI PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Platelets from mice lacking the aryl hydrocarbon receptor exhibit defective collagen-dependent signaling.
Authors: Lindsey S, Jiang J, Woulfe D, Papoutsakis E
J Thromb Haemost, 2014-01-01;12(3):383-94.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry