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Mouse Gr-1/Ly-6G PerCP-conjugated Antibody

Catalog #: FAB1037C
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB1037C-100
FAB1037C-025
Detection of Gr‑1/Ly‑6G in Mouse Splenocytes by Flow Cytometry.
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Citations (3)
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Mouse Gr-1/Ly-6G PerCP-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Gr-1/Ly-6G. Weak cross-reactivity with Ly-6C is observed.
Source
Monoclonal Rat IgG2B Clone # RB6-8C5
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
The immunogen for this antibody is Ly-6G (Gr-1, Gr1).
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
PerCP (Peridinin-chlorophyll Protein Complex) (Excitation= 482 and 564 nm, Emission= 675 nm)
Purity
Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Gr-1/Ly-6G antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of Gr‑1/Ly‑6G in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R PE-conjugated Monoclonal Antibody (Catalog # FAB1217P) and either (A) Rat Anti-Mouse Gr-1/Ly-6G PerCP-conjugated Monoclonal Antibody (Catalog # FAB1037C) or (B) Rat IgG2BPerCP Isotype Control (Catalog # IC013C). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Ly-6G/Ly-6C (Gr-1)

The myeloid differentiation antigen Gr-1 is a member of the Ly-6 family, Ly-6G (1). The RB6‑8C5 antibody also reacts weakly with Ly-6C-transfected EL4 cells (2). In the periphery, this antibody specifically recognized granulocytes (3, 4).

References
  1. Spangrude, G.J. et al. (1988) Science 241:58.
  2. Fleming, T.J. et al. (1993) J. Immunol. 151:2399.
  3. Lewinsohn, D.M. et al.(1987) J. Immunol. 147:22.
  4. Lagasse, E. and I.L. Weissman (1996) J. Immunol. Methods 197:139.
Long Name
A Myeloid Differentiation Antigen
Entrez Gene IDs
546644 (Mouse)
Alternate Names
C6orf23; G6D; Ly-6C; LY6-D; Ly6G; Ly-6G; Ly-6G/Ly-6C (Gr-1); LY6G6D; lymphocyte antigen 6 family member G6D; MEGT1; NG25

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Citations for Mouse Gr-1/Ly-6G PerCP-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Inflammatory and non-inflammatory monocytes as novel prognostic biomarkers of survival in SOD1G93A mouse model of Amyotrophic Lateral Sclerosis
    Authors: S Gasco, P Zaragoza, A García-Red, AC Calvo, R Osta
    PLoS ONE, 2017-09-08;12(9):e0184626.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. IL-12 promotes myeloid-derived suppressor cell recruitment and bacterial persistence during Staphylococcus aureus orthopedic implant infection.
    Authors: Heim C, Vidlak D, Scherr T, Hartman C, Garvin K, Kielian T
    J Immunol, 2015-03-11;194(8):3861-72.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Macrophage colony-stimulating factor augments Tie2-expressing monocyte differentiation, angiogenic function, and recruitment in a mouse model of breast cancer.
    Authors: Forget M, Voorhees J, Cole S, Dakhlallah D, Patterson I, Gross A, Moldovan L, Mo X, Evans R, Marsh C, Eubank T
    PLoS ONE, 2014-06-03;9(6):e98623.
    Species: Mouse
    Sample Types: Whole Blood
    Applications: Functional Assay

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