Mouse ICAM-1/CD54 Antibody

Catalog #: MAB796
6 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB796-SP
MAB796-500
MAB796-100

All ICAM-1/CD54 Antibodies

Product Details

Citations (6)

Supplemental Products

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse ICAM-1/CD54 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse ICAM-1 in ELISAs and Western blots. In Western blots, this antibody shows weak cross-reactivity with recombinant human (rh) ICAM‑1 and rmICAM-2 and no cross-reactivity with rhCD31, rmDCC, rhICAM-2, rhICAM-3, rrICAM-1, rmMAdCAM-1, or rmVCAM-1.

Source

Monoclonal Rat IgG_2B Clone # 166623

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse ICAM-1
Gln28-Asn485
Accession # P13597

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse ICAM‑1/CD54 Fc Chimera (Catalog # 796-IC)

Mouse ICAM-1/CD54 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Mouse ICAM‑1/CD54 Biotinylated Antibody (Catalog # BAF796)

Standard: Recombinant Mouse ICAM-1/CD54 Fc Chimera Protein, CF (Catalog # 796-IC)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ICAM-1/CD54

Intercellular Adhesion Molecule-1 (ICAM-1, CD54) binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma, TNF-alpha, IL-1 beta, and LPS. Mouse and human ICAM-1 share approximately 54% amino acid sequence identity. Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, leukocyte adhesion deficiency syndrome (LAD), cancer, and transplantation.

References

Pigott, R. and C. Power (1993) in The Adhesion Molecule Facts Book. Academic Press, p. 74.
Siu, G. et al. (1989) J. Immunol. 143:3813.
Ballantyne, C.M. et al. (1989) Nuc. Acid. Res. 17:5853.

Long Name

Intercellular Adhesion Molecule 1

Entrez Gene IDs

3383 (Human); 15894 (Mouse); 25464 (Rat)

Alternate Names

BB2; CD54 antigen; CD54; cell surface glycoprotein P3.58; human rhinovirus receptor; ICAM1; ICAM-1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule 1; Major group rhinovirus receptor; P3.58

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse ICAM-1/CD54 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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An Anti-Human ICAM-1 Antibody Inhibits Rhinovirus-Induced Exacerbations of Lung Inflammation
Authors: Stephanie Traub, Alexandra Nikonova, Alan Carruthers, Rebecca Dunmore, Katherine A. Vousden, Leila Gogsadze et al.
PLoS Pathogens
Lactobacillus plantarum reverse diabetes-induced Fmo3 and ICAM expression in mice through enteric dysbiosis-related c-Jun NH2-terminal kinase pathways
Authors: WC Liu, MC Yang, YY Wu, PH Chen, CM Hsu, LW Chen
PLoS ONE, 2018-05-31;13(5):e0196511.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
Response patterns of cytokines/chemokines in two murine strains after irradiation.
Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
Cytokine, 2012-01-25;58(2):169-77.
Species: Mouse
Sample Types: Plasma
Applications: Luminex Development
Overexpression of sICAM-1 in the Alveolar Epithelial Space Results in an Exaggerated Inflammatory Response and Early Death in Gram Negative Pneumonia.
Authors: Mendez MP, Monroy YK, Du M
Respir. Res., 2011-01-19;12(1):12.
Species: Mouse
Sample Types: BALF
Applications: Western Blot
A new RXR agonist, HX630, suppresses intimal hyperplasia in a mouse blood flow cessation model.
Authors: Haraguchi G, Suzuki J, Kosuge H, Ogawa M, Koga N, Muto S, Itai A, Kagechika H, Shudo K, Isobe M
J. Mol. Cell. Cardiol., 2006-09-11;41(5):885-92.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Shedding of soluble ICAM-1 into the alveolar space in murine models of acute lung injury.
Authors: Mendez MP, Morris SB, Wilcoxen S, Greeson E, Moore B, Paine R
Am. J. Physiol. Lung Cell Mol. Physiol., 2005-12-22;290(5):L962-70.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot