Mouse ICAM-1/CD54 Antibody Summary
Gln28-Asn485
Accession # Q3U8M7
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
ICAM‑1/CD54 in Mouse Testis. ICAM-1/CD54 was detected in perfusion fixed frozen sections of mouse testis using Goat Anti-Mouse ICAM-1/CD54 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF796) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in Leydig cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ICAM-1/CD54
Intercellular Adhesion Molecule-1 (ICAM-1, CD54) binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma, TNF-alpha, IL-1 beta, and LPS. Mouse and human ICAM-1 share approximately 54% amino acid identity.
Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, leukocyte adhesion deficiency syndrome (LAD), cancer, and transplantation.
- Pigott, R. and C. Power (1993) in The Adhesion Molecule Facts Book. Academic Press, p. 74.
- Siu, G. et al. (1989) J. Immunol. 143:3813.
- Ballantyne, C.M. et al. (1989) Nuc. Acid. Res. 17:5853.
Product Datasheets
Citations for Mouse ICAM-1/CD54 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Production of Dermatophagoides farinae Having Low Bacterial Content Using Ampicillin
Authors: Kim, JY;Yi, MH;Kim, M;Choi, JH;Lee, S;Yong, TS;
Journal of immunology research
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
TMEM59 ablation leads to loss of olfactory sensory neurons and impairs olfactory functions via interaction with inflammation
Authors: Z Ma, W Li, L Zhuang, T Wen, P Wang, H Yu, Y Liu, Y Yu
Brain, Behavior, and Immunity, 2023-04-13;111(0):151-168.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Lgr5+ cells are required and dynamically participate in olfactory epithelium regeneration: a revisiting shows Lgr5 expression in multiple cell lineages
Authors: W Ren, Z Ma, L Wang, X Feng, H Yu, Y Yu
Theranostics, 2022-07-18;12(13):5631-5644.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Targeted BRD4 protein degradation by dBET1 ameliorates acute ischemic brain injury and improves functional outcomes associated with reduced neuroinflammation and oxidative stress and preservation of blood-brain barrier integrity
Authors: L Liu, C Yang, BP Lavayen, RJ Tishko, J Larochelle, E Candelario
Journal of Neuroinflammation, 2022-06-27;19(1):168.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Novel role for caspase 1 inhibitor VX765 in suppressing NLRP3 inflammasome assembly and atherosclerosis via promoting mitophagy and efferocytosis
Authors: Y Jin, Y Liu, L Xu, J Xu, Y Xiong, Y Peng, K Ding, S Zheng, N Yang, Z Zhang, L Li, L Tan, H Song, J Fu
Cell Death & Disease, 2022-05-31;13(5):512.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Pericyte Loss Leads to Capillary Stalling Through Increased Leukocyte-Endothelial Cell Interaction in the Brain
Authors: YG Choe, JH Yoon, J Joo, B Kim, SP Hong, GY Koh, DS Lee, WY Oh, Y Jeong
Frontiers in Cellular Neuroscience, 2022-03-11;16(0):848764.
Species: Mouse
Sample Types: Protein Lysates
Applications: Western Blot -
Critical changes in hypothalamic gene networks in response to pancreatic cancer as found by single-cell RNA sequencing
Authors: C Huisman, MA Norgard, PR Levasseur, SM Krasnow, MGP van der Wi, B Olson, DL Marks
Molecular Metabolism, 2022-01-11;0(0):101441.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Deficiency of peroxiredoxin 2 exacerbates angiotensin II-induced abdominal aortic aneurysm
Authors: SJ Jeong, MJ Cho, NY Ko, S Kim, IH Jung, JK Min, SH Lee, JG Park, GT Oh
Exp. Mol. Med., 2020-09-14;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
RNA-seq analysis of gene expression profiles in isolated stria vascularis from wild-type and Alport mice reveals key pathways underling Alport strial pathogenesis
Authors: B Dufek, DT Meehan, D Delimont, K Wilhelm, G Samuelson, R Coenen, J Madison, E Doyle, B Smyth, G Phillips, MA Gratton, D Cosgrove
PLoS ONE, 2020-08-21;15(8):e0237907.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Exposure to Zinc Oxide Nanoparticles Disrupts Endothelial Tight and Adherens Junctions and Induces Pulmonary Inflammatory Cell Infiltration
Authors: CM Chen, ML Wu, YC Ho, PY Gung, MH Tsai, AN Orekhov, IA Sobenin, P Lin, SF Yet
Int J Mol Sci, 2020-05-13;21(10):.
Species: Human
Sample Types: Cell Lys
Applications: Western Blot -
A Multivalent ICAM-1 Binding Nanoparticle which Inhibits ICAM-1 and LFA-1 Interaction Represents a New Tool for the Investigation of Autoimmune-Mediated Dry Eye
Authors: PY Hsueh, Y Ju, A Vega, MC Edman, JA MacKay, SF Hamm-Alvar
Int J Mol Sci, 2020-04-15;21(8):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Cell-specific and athero-protective roles for RIPK3 in a murine model of atherosclerosis
Authors: S Colijn, V Muthukumar, J Xie, S Gao, CT Griffin
Dis Model Mech, 2020-01-24;13(1):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
IgA1 deposition may induce NLRP3 expression and macrophage transdifferentiation of podocyte in IgA nephropathy
Authors: W Peng, GQ Pei, Y Tang, L Tan, W Qin
J Transl Med, 2019-12-03;17(1):406.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Interleukin 1 alpha administration is neuroprotective and neuro-restorative following experimental ischemic stroke
Authors: KE Salmeron, ME Maniskas, DN Edwards, R Wong, I Rajkovic, A Trout, AA Rahman, S Hamilton, JF Fraser, E Pinteaux, GJ Bix
J Neuroinflammation, 2019-11-14;16(1):222.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
VCAM-1 Density and Tumor Perfusion Predict T-cell Infiltration and Treatment Response in Preclinical Models
Authors: J Riegler, H Gill, A Ogasawara, M Hedehus, V Javinal, J Oeh, GZ Ferl, J Marik, S Williams, D Sampath, J Schartner, RAD Carano
Neoplasia, 2019-09-11;21(10):1036-1050.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization -
Intermittent Hypoxia Activates Duration-Dependent Protective and Injurious Mechanisms in Mouse Lung Endothelial Cells
Authors: P Wohlrab, L Soto-Gonza, T Benesch, MP Winter, IM Lang, K Markstalle, V Tretter, KU Klein
Front Physiol, 2018-12-06;9(0):1754.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Early and Late Protective Effect of Bone Marrow Mononuclear Cell Transplantation on Radiation-Induced Vascular Dysfunction and Skin Lesions
Authors: V Holler, V Buard, T Roque, C Squiban, M Benderitte, S Flamant, R Tamarat
Cell Transplant, 2018-11-09;0(0):9636897188103.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Neurokinin-1 receptor antagonism improves postoperative neurocognitive disorder in mice.
Authors: Li Z, Luo T, Ning X, Xiong C, Wu A
Neurosci Lett, 2018-09-28;687(0):189-195.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Complement activation contributes to perioperative neurocognitive disorders in mice
Authors: C Xiong, J Liu, D Lin, J Zhang, N Terrando, A Wu
J Neuroinflammation, 2018-09-04;15(1):254.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Corticosterone Production during Repeated Social Defeat Causes Monocyte Mobilization from the Bone Marrow, Glucocorticoid Resistance and Neurovascular Adhesion Molecule Expression
Authors: A Niraula, Y Wang, JP Godbout, JF Sheridan
J. Neurosci., 2018-01-30;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Suberanilohydroxamic Acid as a Pharmacological Kruppel-Like Factor 2 Activator That Represses Vascular Inflammation and Atherosclerosis
Authors: Y Xu, S Xu, P Liu, M Koroleva, S Zhang, S Si, ZG Jin
J Am Heart Assoc, 2017-11-30;6(12):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Interferon-? treatment in vitro elicits some of the changes in cathepsin S and antigen presentation characteristic of lacrimal glands and corneas from the NOD mouse model of Sj�gren's Syndrome
Authors: Z Meng, W Klinngam, MC Edman, SF Hamm-Alvar
PLoS ONE, 2017-09-13;12(9):e0184781.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Nicotinamide mononucleotide attenuates brain injury after intracerebral hemorrhage by activating Nrf2/HO-1 signaling pathway
Authors: CC Wei, YY Kong, GQ Li, YF Guan, P Wang, CY Miao
Sci Rep, 2017-04-06;7(1):717.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Plasminogen Activator Inhibitor-1 Protects Mice Against Cardiac Fibrosis by Inhibiting Urokinase-type Plasminogen Activator-mediated Plasminogen Activation
Authors: KK Gupta, DL Donahue, MJ Sandoval-C, FJ Castellino, VA Ploplis
Sci Rep, 2017-03-23;7(1):365.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Epidermal RAF prevents allergic skin disease
Elife, 2016-07-19;5(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Neutrophil Extracellular Traps Accumulate in Peripheral Blood Vessels and Compromise Organ Function in Tumor-Bearing Animals.
Authors: Cedervall J, Zhang Y, Huang H, Zhang L, Femel J, Dimberg A, Olsson A
Cancer Res, 2015-06-12;75(13):2653-62.
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Platelet-driven leukotriene C4-mediated airway inflammation in mice is aspirin-sensitive and depends on T prostanoid receptors.
Authors: Liu T, Garofalo D, Feng C, Lai J, Katz H, Laidlaw T, Boyce J
J Immunol, 2015-04-22;194(11):5061-8.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Abrogation of plasminogen activator inhibitor-1-vitronectin interaction ameliorates acute kidney injury in murine endotoxemia.
Authors: Gupta K, Donahue D, Sandoval-Cooper M, Castellino F, Ploplis V
PLoS ONE, 2015-03-23;10(3):e0120728.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Low-dose irradiation affects expression of inflammatory markers in the heart of ApoE -/- mice.
Authors: Mathias D, Mitchel R, Barclay M, Wyatt H, Bugden M, Priest N, Whitman S, Scholz M, Hildebrandt G, Kamprad M, Glasow A
PLoS ONE, 2015-03-23;10(3):e0119661.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Intercellular adhesion molecule-1 expression by skeletal muscle cells augments myogenesis.
Authors: Goh Q, Dearth C, Corbett J, Pierre P, Chadee D, Pizza F
Exp Cell Res, 2014-09-30;331(2):292-308.
Species: Mouse
Sample Types: Whole Cells
Applications: IHC-Fr -
ICAM-1-based rabies virus vaccine shows increased infection and activation of primary murine B cells in vitro and enhanced antibody titers in-vivo.
Authors: Norton, James E, Lytle, Andrew G, Shen, Shixue, Tzvetkov, Evgeni P, Dorfmeier, Corin L, McGettigan, James P
PLoS ONE, 2014-01-29;9(1):e87098.
Species: Hamster
Sample Types: Cell Lysates
Applications: Western Blot -
CD44-deficiency attenuates the immunologic responses to LPS and delays the onset of endotoxic shock-induced renal inflammation and dysfunction.
Authors: Rampanelli, Elena, Dessing, Mark C, Claessen, Nike, Teske, Gwendoli, Joosten, Sander P, Pals, Steven T, Leemans, Jaklien, Florquin, Sandrine
PLoS ONE, 2013-12-23;8(12):e84479.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Peptide inhibitor of NF-kappaB translocation ameliorates experimental atherosclerosis.
Authors: Mallavia B, Recio C, Oguiza A, Ortiz-Munoz G, Lazaro I, Lopez-Parra V, Lopez-Franco O, Schindler S, Depping R, Egido J, Gomez-Guerrero C
Am J Pathol, 2013-04-16;182(5):1910-21.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Skeletal muscle cells express ICAM-1 after muscle overload and ICAM-1 contributes to the ensuing hypertrophic response.
Authors: Dearth C, Goh Q, Marino J, Cicinelli P, Torres-Palsa M, Pierre P, Worth R, Pizza F
PLoS ONE, 2013-03-11;8(3):e58486.
Species: Mouse
Sample Types: Cell Lysates, Whole Cells, Whole Tissue
Applications: ICC, IHC, Western Blot -
Biomarkers of disease and treatment in murine and cynomolgus models of chronic asthma.
Authors: Louten J, Mattson JD, Malinao MC, Li Y, Emson C, Vega F, Wardle RL, Van Scott MR, Fick RB, McClanahan TK, de Waal Malefyt R, Beaumont M
Biomark Insights, 2012-07-10;7(0):87-104.
Species: Mouse
Sample Types: BALF
Applications: Western Blot -
Lung-targeted overexpression of the NF-kappaB member RelB inhibits cigarette smoke-induced inflammation.
Authors: McMillan DH, Baglole CJ, Thatcher TH, Maggirwar S, Sime PJ, Phipps RP
Am. J. Pathol., 2011-05-05;179(1):125-33.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Increased expression of CXCR4 and integrin alphaM in hypoxia-preconditioned cells contributes to improved cell retention and angiogenic potency.
Authors: Kubo M, Li TS, Kamota T, Ohshima M, Qin SL, Hamano K
J. Cell. Physiol., 2009-08-01;220(2):508-14.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
The E-selectin ligand basigin/CD147 is responsible for neutrophil recruitment in renal ischemia/reperfusion.
Authors: Kato N, Yuzawa Y, Kosugi T
J. Am. Soc. Nephrol., 2009-05-14;20(7):1565-76.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC-Fr, Western Blot -
Apelin prevents aortic aneurysm formation by inhibiting macrophage inflammation.
Authors: Leeper NJ, Tedesco MM, Kojima Y, Schultz GM, Kundu RK, Ashley EA, Tsao PS, Dalman RL, Quertermous T
Am. J. Physiol. Heart Circ. Physiol., 2009-03-20;296(5):H1329-35.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Antigen-loaded ER microsomes from APC induce potent immune responses against viral infection.
Authors: Sofra V, Mansour S, Liu M, Gao B, Primpidou E, Wang P, Li S
Eur. J. Immunol., 2009-01-01;39(1):85-95.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
PPARgamma agonist rosiglitazone is neuroprotective after traumatic brain injury via anti-inflammatory and anti-oxidative mechanisms.
Authors: Yi JH, Park SW, Brooks N, Lang BT, Vemuganti R
Brain Res., 2008-10-09;1244(0):164-72.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Plasminogen activator inhibitor-1 regulates neutrophil influx during acute pyelonephritis.
Authors: Roelofs JJ, Teske GJ, Bonta PI, de Vries CJ, Meijers JC, Weening JJ, van der Poll T, Florquin S
Kidney Int., 2008-09-17;75(1):52-9.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Vascular inflammation, insulin resistance, and reduced nitric oxide production precede the onset of peripheral insulin resistance.
Authors: Kim F, Pham M, Maloney E, Rizzo NO, Morton GJ, Wisse BE, Kirk EA, Chait A, Schwartz MW
Arterioscler. Thromb. Vasc. Biol., 2008-09-04;28(11):1982-8.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Atherosclerosis prevention by a fish oil-rich diet in apoE(-/-) mice is associated with a reduction of endothelial adhesion molecules.
Authors: Casos K, Saiz MP, Ruiz-Sanz JI, Mitjavila MT
Atherosclerosis, 2008-03-16;201(2):306-17.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Dietary alpha-lipoic acid supplementation inhibits atherosclerotic lesion development in apolipoprotein E-deficient and apolipoprotein E/low-density lipoprotein receptor-deficient mice.
Authors: Zhang WJ, Bird KE, McMillen TS, LeBoeuf RC, Hagen TM, Frei B
Circulation, 2007-12-24;117(3):421-8.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Fractalkine upregulates intercellular adhesion molecule-1 in endothelial cells through CX3CR1 and the Jak Stat5 pathway.
Authors: Yang XP, Mattagajasingh S, Su S, Chen G, Cai Z, Fox-Talbot K, Irani K, Becker LC
Circ. Res., 2007-09-20;101(10):1001-8.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Crosstalk between keratinocytes and adaptive immune cells in an IkappaBalpha protein-mediated inflammatory disease of the skin.
Authors: Rebholz B, Haase I, Eckelt B, Paxian S, Flaig MJ, Ghoreschi K, Nedospasov SA, Mailhammer R, Debey-Pascher S, Schultze JL, Weindl G, Forster I, Huss R, Stratis A, Ruzicka T, Rocken M, Pfeffer K, Schmid RM, Rupec RA
Immunity, 2007-08-09;27(2):296-307.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
High pressure promotes monocyte adhesion to the vascular wall.
Authors: Riou S, Mees B, Esposito B, Merval R, Vilar J, Stengel D, Ninio E, van Haperen R, de Crom R, Tedgui A, Lehoux S
Circ. Res., 2007-03-29;100(8):1226-33.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
An inflammation-induced mechanism for leukocyte transmigration across lymphatic vessel endothelium.
Authors: Johnson LA, Clasper S, Holt AP, Lalor PF, Baban D, Jackson DG
J. Exp. Med., 2006-11-20;203(12):2763-77.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Activation of mouse protease-activated receptor-2 induces lymphocyte adhesion and generation of reactive oxygen species.
Authors: Lim SY, Tennant GM, Kennedy S, Wainwright CL, Kane KA
Br. J. Pharmacol., 2006-09-18;149(5):591-9.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Shedding of soluble ICAM-1 into the alveolar space in murine models of acute lung injury.
Authors: Mendez MP, Morris SB, Wilcoxen S, Greeson E, Moore B, Paine R
Am. J. Physiol. Lung Cell Mol. Physiol., 2005-12-22;290(5):L962-70.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P
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