Mouse ICOS Antibody

Catalog #: AF168 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF168
AF168-SP

All ICOS Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse ICOS Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse ICOS in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross‑reactivity with recombinant mouse (rm) CTLA-4, rmCD28, and rmPD-1 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse ICOS
Met1-Leu142
Accession # CAB71153

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse ICOS Fc Chimera (Catalog # 168-CS)

Blockade of Receptor-ligand Interaction

In a functional ELISA, 10-40 µg/mL of this antibody will block 50% of the binding of 500 ng/mL of Biotinylated Recombinant Human B7-H2 Fc Chimera to immobilized Recombinant Mouse ICOS (Catalog # 168-CS) coated at 1 µg/mL (100 µL/well). At 75 μg/mL, this antibody will block >90% of the binding.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ICOS

Mouse ICOS (inducible co-stimulator), also called AILIM (activiation-inducible lymphocyte immunomediatory molecule) and CRP-1 (CD28-related protein-1), is a member of the CD28 family of immune costimulatory receptors. Other family members are CD28, CTLA-4 and PD-1. Mouse ICOS is a homodimeric type I transmembrane protein consisting of 200 amino acids (aa) with a putative 20 aa signal sequence, a 122 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain. ICOS shares approximately 39% aa similarity with CD 28 and CTLA-4. Mouse and human ICOS share approximately 72% aa identity. ICOS is expressed on most CD45RO⁺ cells. ICOS expression is upregulated within approximately 24‑48 hours of activation on T_h primed cells. B7-H2, a member of the B7 family of costimulatory ligands, has been identified as the ICOS ligand. The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and T_h differentiation.

References

Coyle, A.J. and J.C. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
Mages, H.W. et al. (2000) Eur. J. Immunol. 30:1040.
Yoshinaga, S.K. et al. (1999) Nature 402:827.
Hutloff, A. et al. (1999) Nature 397:263.
Aicher, A. et al. (2000) J. Immunol. 164:4689.
Coyle, A.J. et al. (2000) Immunity 13:95.
Gonzalo, J.A. et al. (2001) J. Immunol. 166:1.

Long Name

Inducible Co-stimulator

Entrez Gene IDs

29851 (Human); 54167 (Mouse); 102116512 (Cynomolgus Monkey)

Alternate Names

Activation-inducible lymphocyte immunomediatory molecule; AILIM; AILIMCD278; CD278 antigen; CD278; CRP-1; CVID1; ICOS; inducible costimulator; inducible T-cell costimulator; inducible T-cell co-stimulator; MGC39850