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Mouse IGF-I/IGF-1 Antibody

Catalog #: AF791
21 Citations 1 Review Datasheet / COA / SDS
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AF791
AF791-SP
Cell Proliferation Induced by IGF-I/IGF-1 and Neutralization by Mouse IGF-I/IGF-1 Antibody.
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Citations (21)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IGF-I/IGF-1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IGF-I/IGF-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human IGF-I/IGF-1 is observed, and less than 2% cross-reactivity with recombinant mouse IGF-II/IGF-2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse IGF-I/IGF-1
Gl33-Ala102
Accession # Q8CAR0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse IGF-I/IGF-1 (Catalog # 791-MG)
Immunohistochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize IGF-I/IGF-1-induced proliferation in the MCF‑7 human breast cancer cell line. Karey, K. P. et al. (1988) Cancer Research 48:4083. The Neutralization Dose (ND50) is typically 0.2-1.0 µg/mL in the presence of 15 ng/mL Recombinant Mouse IGF-I/IGF-1.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by IGF-I/IGF-1 and Neutralization by Mouse IGF-I/IGF-1 Antibody. View Larger

Cell Proliferation Induced by IGF-I/IGF-1 and Neutralization by Mouse IGF-I/IGF-1 Antibody. Recombinant Mouse IGF-I/IGF-1 (Catalog # 791-MG) stimulates proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IGF-I/IGF-1 (15 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Mouse IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF791). The ND50 is typically 0.2-1.0 µg/mL.

Immunohistochemistry IGF-I/IGF-1 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

IGF-I/IGF-1 in Mouse Embryo. IGF-I/IGF-1 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF791) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to developing brain and muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGF-I/IGF-1

Insulin-like growth factor I, also known as somatomedin C, is the dominant effector of growth hormone and is structurally homologous to proinsulin. Mouse IGF-I is synthesized as two precursor isoforms with alternate N- and C-terminal propeptides (1). These isoforms are differentially expressed by various tissues (1). The 7.6 kDa mature IGF-I is identical between isoforms and is generated by proteolytic removal of the N- and C-terminal regions. Mature mouse IGF-I shares 94% and 99% aa sequence identity with human and rat IGF-I, respectively (2), and exhibits cross-species activity. It shares 60% aa sequence identity with mature mouse IGF‑II. Circulating IGF-I is produced by hepatocytes, while local IGF-I is produced by many other tissues in which it has paracrine effects (1). IGF-I induces the proliferation, migration, and differentiation of a wide variety of cell types during development and postnatally (3). IGF-I regulates glucose and fatty acid metabolism, steroid hormone activity, and cartilage and bone metabolism (4-7). It plays an important role in muscle regeneration and tumor progression (1, 8). IGF-I binds IGF-I R, IGF-II R, and the insulin receptor, although its effects are mediated primarily by IGF-I R (9). IGF-I association with IGF binding proteins increases its plasma half-life and modulates its interactions with receptors (10).

References
  1. Philippou, A. et al. (2007) In Vivo 21:45.
  2. Bell, G.I. et al. (1986) Nucleic Acids Res. 14:7873.
  3. Guvakova, M.A. (2007) Int. J. Biochem. Cell Biol. 39:890.
  4. Clemmons, D.R. (2006) Curr. Opin. Pharmacol. 6:620.
  5. Bluher, S. et al. (2005) Best Pract. Res. Clin. Endocrinol. Metab. 19:577.
  6. Garcia-Segura, L.M. et al. (2006) Neuroendocrinology 84:275.
  7. Malemud, C.J. (2007) Clin. Chim. Acta 375:10.
  8. Samani, A.A. et al. (2007) Endocrine Rev. 28:20.
  9. LeRoith, D. and S. Yakar (2007) Nat. Clin. Pract. Endocrinol. Metab. 3:302.
  10. Denley, A. et al. (2005) Cytokine Growth Factor Rev. 16:421.
Long Name
Insulin-like Growth Factor I/Insulin-like Growth Factor 1
Entrez Gene IDs
3479 (Human); 16000 (Mouse); 24482 (Rat)
Alternate Names
IBP1; IGF1; IGF-1; IGF1A; IGFI; IGF-I; IGF-IA; IGF-IB; insulin-like growth factor 1 (somatomedin C); insulin-like growth factor 1; insulin-like growth factor I; insulin-like growth factor IA; insulin-like growth factor IB; Mechano growth factor; MGF; Somatomedin A; Somatomedin C; somatomedin-C

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Citations for Mouse IGF-I/IGF-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

21 Citations: Showing 1 - 10
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  1. Bone marrow-derived IGF-1 orchestrates maintenance and regeneration of the adult skeleton
    Authors: J Wang, Q Zhu, D Cao, Q Peng, X Zhang, C Li, C Zhang, BO Zhou, R Yue
    Proceedings of the National Academy of Sciences of the United States of America, 2022-12-28;120(1):e2203779120.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  2. Single-cell transcriptome analyses reveal microglia types associated with proliferative retinopathy
    Authors: Z Liu, H Shi, J Xu, Q Yang, Q Ma, X Mao, Z Xu, Y Zhou, Q Da, Y Cai, DJ Fulton, Z Dong, A Sodhi, RB Caldwell, Y Huo
    JCI Insight, 2022-12-08;7(23):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC/IF
  3. Microglia-neuron interaction at nodes of Ranvier depends on neuronal activity through potassium release and contributes to remyelination
    Authors: R Ronzano, T Roux, M Thetiot, MS Aigrot, L Richard, FX Lejeune, E Mazuir, JM Vallat, C Lubetzki, A Desmazière
    Nature Communications, 2021-09-01;12(1):5219.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Muscle insulin-like growth factor-I modulates murine craniofacial bone growth
    Authors: HJ Kok, CN Crowder, L Koo Min Ch, HY Choi, N Lin, ER Barton
    European cells & materials, 2021-07-19;41(0):72-89.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  5. Lacrimal gland budding requires PI3K-dependent suppression of EGF signaling
    Authors: Wang Q, Tao C, Hannan A et al.
    Science Advances
  6. Forceful mastication activates osteocytes and builds a stout jawbone
    Authors: M Inoue, T Ono, Y Kameo, F Sasaki, T Ono, T Adachi, T Nakashima
    Sci Rep, 2019-03-20;9(1):4404.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: IHC-P, Neutralization
  7. Microglia are an essential component of the neuroprotective scar that forms after spinal cord injury
    Authors: V Bellver-La, F Bretheau, B Mailhot, N Vallières, M Lessard, ME Janelle, N Vernoux, MÈ Tremblay, T Fuehrmann, MS Shoichet, S Lacroix
    Nat Commun, 2019-01-31;10(1):518.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. Obesity-Activated Adipose-Derived Stromal Cells Promote Breast Cancer Growth and Invasion
    Authors: LE Hillers, JV D'Amato, T Chamberlin, G Paderta, LM Arendt
    Neoplasia, 2018-10-12;20(11):1161-1174.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: Neutralization, Western Blot
  9. AHNAK Loss in Mice Promotes Type II Pneumocyte Hyperplasia and Lung Tumor Development
    Authors: JW Park, IY Kim, JW Choi, HJ Lim, JH Shin, YN Kim, SH Lee, Y Son, M Sohn, JK Woo, JH Jeong, C Lee, YS Bae, JK Seong
    Mol. Cancer Res., 2018-05-03;0(0):.
    Species: Mouse
    Sample Types: Tissue Micorarray
    Applications: IHC
  10. Systemic IGF-1 gene delivery by rAAV9 improves spontaneous autoimmune peripheral polyneuropathy (SAPP)
    Authors: T Gao, N Bogdanova, S Ghauri, G Zhang, J Lin, K Sheikh
    Sci Rep, 2018-04-03;8(1):5408.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. Immunization with Bacillus Calmette-Gu�rin (BCG) alleviates neuroinflammation and cognitive deficits in APP/PS1 mice via the recruitment of inflammation-resolving monocytes to the brain
    Authors: Z Zuo, F Qi, J Yang, X Wang, Y Wu, Y Wen, Q Yuan, J Zou, K Guo, ZB Yao
    Neurobiol. Dis, 2017-02-09;101(0):27-39.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  12. Combined Effects of Androgen and Growth Hormone on Osteoblast Marker Expression in Mouse C2C12 and MC3T3-E1 Cells Induced by Bone Morphogenetic Protein
    Authors: K Kimura, T Terasaka, N Iwata, T Katsuyama, M Komatsubar, R Nagao, K Inagaki, F Otsuka
    J Clin Med, 2017-01-05;6(1):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  13. Endothelial Cords Promote Tumor Initial Growth prior to Vascular Function through a Paracrine Mechanism
    Authors: C Zhao, W Zhang, Y Zhao, Y Yang, H Luo, G Ji, E Dong, H Deng, S Lin, Y Wei, H Yang
    Sci Rep, 2016-01-14;6(0):19404.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  14. Survival of neural stem cell grafts in the lesioned spinal cord is enhanced by a combination of treadmill locomotor training via insulin-like growth factor-1 signaling.
    Authors: Hwang D, Shin H, Kwon M, Choi J, Ryu B, Kim B
    J Neurosci, 2014-09-17;34(38):12788-800.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  15. Viral expression of insulin-like growth factor I E-peptides increases skeletal muscle mass but at the expense of strength.
    Authors: Brisson, Becky K, Spinazzola, Janelle, Park, SooHyun, Barton, Elisabet
    Am J Physiol Endocrinol Metab, 2014-02-25;306(8):E965-74.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  16. Attenuation of AD-like neuropathology by harnessing peripheral immune cells: local elevation of IL-10 and MMP-9.
    Authors: Koronyo-Hamaoui M, Ko MK, Koronyo Y, Azoulay D, Seksenyan A, Kunis G, Pham M, Bakhsheshian J, Rogeri P, Black KL, Farkas DL, Schwartz M
    J. Neurochem., 2009-09-24;111(6):1409-24.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  17. Role of IGF signaling in olfactory sensory map formation and axon guidance.
    Authors: Scolnick JA, Cui K, Duggan CD, Xuan S, Yuan XB, Efstratiadis A, Ngai J
    Neuron, 2008-03-27;57(6):847-57.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  18. Low-dose GH supplementation reduces the TLR2 and TNF-alpha expressions in visceral fat.
    Authors: Kubota Y, Unoki H, Bujo H, Rikihisa N, Udagawa A, Yoshimoto S, Ichinose M, Saito Y
    Biochem. Biophys. Res. Commun., 2008-01-15;368(1):81-7.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  19. Glatiramer acetate fights against Alzheimer's disease by inducing dendritic-like microglia expressing insulin-like growth factor 1.
    Authors: Butovsky O, Koronyo-Hamaoui M, Kunis G, Ophir E, Landa G, Cohen H, Schwartz M
    Proc. Natl. Acad. Sci. U.S.A., 2006-07-24;103(31):11784-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  20. Induction and blockage of oligodendrogenesis by differently activated microglia in an animal model of multiple sclerosis.
    Authors: Butovsky O, Landa G, Kunis G, Ziv Y, Avidan H, Greenberg N, Schwartz A, Smirnov I, Pollack A, Jung S, Schwartz M
    J. Clin. Invest., 2006-03-23;116(4):905-15.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC-Fr
  21. Microglia activated by IL-4 or IFN-gamma differentially induce neurogenesis and oligodendrogenesis from adult stem/progenitor cells.
    Authors: Butovsky O, Ziv Y, Schwartz A, Landa G, Talpalar AE, Pluchino S, Martino G, Schwartz M
    Mol. Cell. Neurosci., 2005-11-16;31(1):149-60.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC, Neutralization

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Mouse IGF-I/IGF-1 Antibody
By Rika Ohkubo on 04/18/2019
Application: WB Sample Tested: Purified protein Species: Mouse

Could not detect IGF-1 when I used liver samples from mice.

Western Blot Mouse IGF-I/IGF-1 Antibody AF791