Mouse IGFBP-5 Biotinylated Antibody

Catalog #: BAF578 Datasheet / COA / SDS

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BAF578

All IGFBP-5 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IGFBP-5 Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IGFBP-5 in ELISAs and Western blots. In sandwich immunoassays, less than 3% cross-reactivity with recombinant human (rh) IGFBP-5 is observed and 0.3% cross-reactivity with recombinant mouse (rm) IGF-I, rmIGF-II, rmIGFBP-I, rmIGFBP-2, rmIGFBP-3, and rmIGFBP-6 is observed. In Western blots, approximately 15% cross-reactivity with rhIGFBP-5 is observed and less than 1% cross-reactivity with rmIGFBP-1, rmIGFBP-2, rmIGFBP-3, and rmIGFBP-6 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse IGFBP-5
Val15-Glu271
Accession # Q3UQV0

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse IGFBP‑5 (Catalog # 578-B5)

Mouse IGFBP-5 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Mouse IGFBP‑5 Antibody (Catalog # AF578)

Standard: Recombinant Mouse IGFBP-5 Protein, CF (Catalog # 578-B5)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGFBP-5

The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-translational modifications of IGFBPs, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF.

Mouse IGFBP-5 cDNA encodes a 271 amino acid (aa) residue precursor protein with a putative 19 aa residue signal peptide that is processed to generate the 252 aa residue mature protein. Mouse, human and rat IGFBP-5 share 97% identity. IGFBP-5 is expressed by fibroblasts, myoblasts and osteoblasts, making it the predominant IGFBP found in bone extracts. IGFBP-5 has a strong affinity for hydroxyapatite, allowing it to bind to bone cells. When bound to extracelluar matrix, IGFBP-5 is protected from proteolysis and potentiates IGF activity, but when it is soluble, IGFBP-5 is cleaved to a biologically inactive 21 kDa fragment.

References