Mouse IL-11 R alpha Biotinylated Antibody

Catalog #: BAF490 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
BAF490

Product Details

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IL-11 R alpha Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IL-11 R alpha in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse IL-11 R alpha
Met1-Gln367
Accession # Q64385

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse IL‑11 R alpha Fc Chimera (Catalog # 490-IR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-11 R alpha

Mouse interleukin 11 receptor alpha (IL-11 R alpha ), originally designated NR1, is a member of the hematopoietic cytokine receptor family that was cloned on the basis of its conserved WSXWS motif. IL-11 R alpha cDNA encodes a 432 amino acid (aa) residue precursor type I membrane protein with a 23 aa residue signal peptide, a 344 aa residue extracellular domain containing two potential glycosylation sites, a 26 aa residue transmembrane region and a short (39 aa residue) cytoplasmic domain. In comparison with other hematopoietic cytokine receptors, the extracellular domain of IL-11 R alpha is closely related to that of IL-6 R alpha, CNTF R alpha and the p40 subunit of IL‑12, exhibiting 24%, 22% and 16% amino acid sequence identity, respectively. By itself, IL-11 R alpha binds IL-11 with low affinity. IL-11 R alpha, together with gp130, forms a functional high-affinity receptor complex for IL-11. The expression of IL-11 R alpha has been detected in all adult tissues examined (lung, stomach, intestine), during embryonic development and in totipotent and differentiating embryonic stem cells. Recombinant soluble IL-11 R alpha has been shown to mediate IL-11 responsiveness in cells expressing the gp130 molecule. In cells expressing transmembrane IL-11 R alpha and gp130, soluble IL-11 R alpha has been shown to act as an IL-11 antagonist.

References