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Mouse IL-12 p70 Quantikine ELISA Kit

Catalog #: M1270
112 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
PM1270
SM1270
M1270-1
Mouse IL-12 p70 ELISA Linearity
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Citations (112)
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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Mouse IL-12 p70 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL)
Sensitivity
2.5 pg/mL
Assay Range
7.8 - 500 pg/mL (Cell Culture Supernates, Serum)
Specificity
Natural and recombinant mouse IL-12 p70
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse IL-12 p70 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IL-12 p70 in cell culture supernates and mouse serum. It contains recombinant mouse IL-12 expressed in Sf 21 insect cells using a baculovirus expression vector and antibodies raised against recombinant mouse IL-12. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse IL-12 showed linear curves that were parallel to the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-12 p70.

Precision

Intra-Assay Precision (Precision within an assay)
Inter-Assay Precision (Precision between assays)

Cell Culture Supernates, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 36 44 36
Mean (pg/mL) 28 68 193 20 56 230
Standard Deviation 2.5 6.5 19.4 2.3 6.2 20
CV% 8.9 9.6 10.1 11.5 11.1 8.7

Recovery

The recovery of mouse IL-12 p70 spiked to three levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=7) 101 95-106
Serum (n=7) 97 88-104

Linearity

To assess the linearity of the assay, samples containing and/or spiked with various concentrations of mouse IL-12 p70 in each matrix were diluted with Calibrator Diluent and assayed.
Mouse IL-12 p70 ELISA Linearity

Scientific Data

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Mouse IL-12 p70 ELISA Standard Curve

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Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-12 p70

Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. The IL-12 p40 subunit is shared by IL-23, another heterodimeric cytokine that has biological activities similar to, as well as distinct from, IL-12. IL-12 is produced by macrophages and B cells and has been shown to have multiple effects on T cells and natural killer (NK) cells. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on mouse cells.

Long Name:
Interleukin 12 p70
Entrez Gene IDs:
3592 (Human); 16159 (Mouse); 84405 (Rat)
Alternate Names:
CLMF p35; Cytotoxic lymphocyte maturation factor 35 kDa subunit; IL12 p70; IL-12 p70; IL-12 subunit p35; IL-12, subunit p35; interleukin 12, p35; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35); interleukin-12 alpha chain; interleukin-12 subunit alpha; natural killer cell stimulatory factor 1, 35 kD subunit; NF cell stimulatory factor chain 1; NK cell stimulatory factor chain 1; NKSF1; p35
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 50 µL Assay Diluent
  3.   Add 50 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
    6. 100 µL Conjugate
  6.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate and wash 5 times.
    8. 100 µL Substrate Solution
  8.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    9. 100 µL Stop Solution
  9.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Mouse IL-12 p70 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

112 Citations: Showing 1 - 10
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  1. Fine tuning of CpG spatial distribution with DNA origami for improved cancer vaccination
    Authors: Zeng, YC;Young, OJ;Wintersinger, CM;Anastassacos, FM;MacDonald, JI;Isinelli, G;Dellacherie, MO;Sobral, M;Bai, H;Graveline, AR;Vernet, A;Sanchez, M;Mulligan, K;Choi, Y;Ferrante, TC;Keskin, DB;Fell, GG;Neuberg, D;Wu, CJ;Mooney, DJ;Kwon, IC;Ryu, JH;Shih, WM;
    Nature nanotechnology
    Species: Murine polyomavirus strain A3
    Sample Types: Cell Culture Supernates
  2. Decrease in Heparan Sulphate Binding in Tropism-Retargeted Oncolytic Herpes Simplex Virus (ReHV) Delays Blood Clearance and Improves Systemic Anticancer Efficacy
    Authors: Vannini, A;Parenti, F;Forghieri, C;Vannini, G;Barboni, C;Zaghini, A;Gianni, T;Campadelli-Fiume, G;
    Cancers
    Species: Murine polyomavirus strain A3
    Sample Types: Cell Culture Supernates
  3. The antimicrobial peptide cathelicidin drives development of experimental autoimmune encephalomyelitis in mice by affecting Th17 differentiation
    Authors: KJ Smith, D Minns, BJ McHugh, RK Holloway, R O'Connor, A Williams, L Melrose, R McPherson, VE Miron, DJ Davidson, E Gwyer Find
    PloS Biology, 2022-08-26;20(8):e3001554.
    Species: Mouse
    Sample Types: CSF
  4. Activation of TLR-pathway to induce host Th1 immune response against visceral leishmaniasis: Involvement of galactosylated-flavonoids
    Authors: S Pradhan, Snehlata, D Manna, S Karmakar, MK Singh, A Bhattachar, B Mukherjee, J Paul
    Heliyon, 2022-07-03;8(7):e09868.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  5. Liposomal beta-Sitosterol Suppresses Metastasis of CT26/luc Colon Carcinoma via Inhibition of MMP-9 and Evoke of Immune System
    Authors: CY Shen, CF Lee, WT Chou, JJ Hwang, YS Tyan, HY Chuang
    Pharmaceutics, 2022-06-07;14(6):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  6. Functional insights into Brucella transcriptional regulator ArsR
    Authors: C Shi, S Wang, J Han, L Xi, M Li, Z Li, H Zhang
    Microbial pathogenesis, 2022-05-24;168(0):105557.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  7. Nociceptor-derived Reg3gamma prevents endotoxic death by targeting kynurenine pathway in microglia
    Authors: E Sugisawa, T Kondo, Y Kumagai, H Kato, Y Takayama, K Isohashi, E Shimosegaw, N Takemura, Y Hayashi, T Sasaki, MM Martino, M Tominaga, K Maruyama
    Cell Reports, 2022-03-08;38(10):110462.
    Species: Mouse
    Sample Types: Plasma
  8. Engineering immunoproteasome-expressing mesenchymal stromal cells: A potent cellular vaccine for lymphoma and melanoma in mice
    Authors: Jamilah Abusarah, Fatemeh Khodayarian, Nehme El-Hachem, Natasha Salame, Martin Olivier, Mohammad Balood, Katiane Roversi, Sebastien Talbot, Jean-Pierre Bikorimana, Jingkui Chen, Mario Jolicoeur, Louis-Eric Trudeau, Samaneh Kamyabiazar, Borhane Annabi, Francis Robert, Jerry Pelletier, Abed-El-Hakim El-Kadiry, Riam Shammaa, Moutih Rafei
    Cell Rep Med
    Species: Murine polyomavirus strain A3
    Sample Types: Cell Culture Supernates
  9. Non-Clinical In Vitro Evaluation of Antibiotic Resistance Gene-Free Plasmids Encoding Human or Murine IL-12 Intended for First-in-Human Clinical Study
    Authors: S Kos, M Bosnjak, T Jesenko, B Markelc, U Kamensek, K Znidar, U Matkovic, A Rencelj, G Sersa, R Hudej, A Tuljak, M Peterka, M Cemazar
    Pharmaceutics, 2021-10-19;13(10):.
    Species: Mouse
    Sample Types: Cell Cultue Supernates
  10. Mechanism of chlorogenic acid in alveolar macrophage polarization in Klebsiella pneumoniae-induced pneumonia
    Authors: QR Li, SR Tan, L Yang, W He, L Chen, FX Shen, Z Wang, HF Wang
    Journal of leukocyte biology, 2021-09-29;0(0):.
    Species: Mouse
    Sample Types: BALF
  11. Pore-forming alpha-hemolysin efficiently improves the immunogenicity and protective efficacy of protein antigens
    Authors: JT Zou, HM Jing, Y Yuan, LH Lei, ZF Chen, Q Gou, QS Xiong, XL Zhang, Z Zhao, XK Zhang, H Zeng, QM Zou, JY Zhang
    PloS Pathogens, 2021-07-21;17(7):e1009752.
    Species: Mouse
    Sample Types: Tissue Homogenates
  12. Nanoparticle-mediated tumor cell expression of mIL-12 via systemic gene delivery treats syngeneic models of murine lung cancers
    Authors: HH Ahn, C Carrington, Y Hu, HW Liu, C Ng, H Nam, A Park, C Stace, W West, HQ Mao, MG Pomper, CG Ullman, I Minn
    Scientific Reports, 2021-05-06;11(1):9733.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  13. Phagocytosis and macrophage polarization on bacterially contaminated dental implant materials and effects on tissue integration
    Authors: Y Yuan, Y Ren, M Dijk, GI Geertsema-, J Atema-Smit, HJ Busscher, HC van der Me
    European cells & materials, 2021-03-30;41(0):421-430.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  14. Oral administration of eugenol oleate cures experimental visceral leishmaniasis through cytokines abundance
    Authors: MR Charan Raj, A Kar, S Srinivasan, D Chellappan, J Debnath, S Kar Mahapa
    Cytokine, 2020-10-28;0(0):155301.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  15. Zinc Finger Protein St18 Protects against Septic Death by Inhibiting VEGF-A from Macrophages
    Authors: K Maruyama, H Kidoya, N Takemura, E Sugisawa, O Takeuchi, T Kondo, MMA Eid, H Tanaka, MM Martino, N Takakura, Y Takayama, S Akira, A Vandenbon, Y Kumagai
    Cell Rep, 2020-07-14;32(2):107906.
    Species: Mouse
    Sample Types: Serum
  16. Combined administration of SHP2 inhibitor SHP099 and the &alpha7nAChR agonist PNU282987 protect mice against DSS?induced colitis
    Authors: J Xiao, G Zhang, S Gao, J Shen, H Feng, Z He, C Xu
    Mol Med Rep, 2020-07-10;22(3):2235-2244.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  17. Requirements for the differentiation of innate T-bethigh memory-phenotype CD4+ T lymphocytes under steady state
    Authors: T Kawabe, J Yi, A Kawajiri, K Hilligan, D Fang, N Ishii, H Yamane, J Zhu, D Jankovic, KS Kim, G Trinchieri, A Sher
    Nat Commun, 2020-07-06;11(1):3366.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  18. Sphingosine-1-Phosphate Receptor 1 Activity Promotes Tumor Growth by Amplifying VEGF-VEGFR2 Angiogenic Signaling
    Authors: VA Balaji Rag, M Anwar, MZ Akhter, A Chavez, Y Mao, V Natarajan, S Lakshmikan, M Chrzanowsk, AZ Dudek, L Claesson-W, JK Kitajewski, KK Wary, AB Malik, D Mehta
    Cell Rep, 2019-12-10;29(11):3472-3487.e4.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  19. Integrated Cross-Species Analysis Identifies a Conserved Transitional Dendritic Cell Population
    Authors: R Leylek, M Alcántara-, Z Lanzar, A Lüdtke, OA Perez, B Reizis, J Idoyaga
    Cell Rep, 2019-12-10;29(11):3736-3750.e8.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  20. Downregulation of RalGTPase-activating protein promotes colitis-associated cancer via NLRP3 inflammasome activation
    Authors: T Iida, Daisuke Hi, N Minami, M Matsuura, K Wagatsuma, K Kawakami, K Nagaishi, M Nojima, H Ikeuchi, S Hirota, R Shirakawa, H Horiuchi, H Nakase
    Cell Mol Gastroenterol Hepatol, 2019-10-15;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  21. Oncolytic activity of HF10 in head and neck squamous cell carcinomas
    Authors: S Esaki, F Goshima, H Ozaki, G Takano, Y Hatano, D Kawakita, K Ijichi, T Watanabe, Y Sato, T Murata, H Iwata, Y Shibamoto, S Murakami, Y Nishiyama, H Kimura
    Cancer Gene Ther., 2019-09-03;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  22. Emodin regulates neutrophil phenotypes to prevent hypercoagulation and lung carcinogenesis
    Authors: Z Li, Y Lin, S Zhang, L Zhou, G Yan, Y Wang, M Zhang, M Wang, H Lin, Q Tong, Y Duan, G Du
    J Transl Med, 2019-03-18;17(1):90.
    Species: Mouse
    Sample Types: Alveolar Fluid
  23. Dendritic cells modulate c-kit expression on the edge between activation and death
    Authors: S Simonetti, ABB Seijas, A Natalini, S Vitale, D Runci, A Soriani, A Di Virgili, E Aricò, L Gabriele, A Santoni, F Di Rosa
    Eur. J. Immunol., 2019-02-25;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  24. Tumoral NOX4 recruits M2 tumor-associated macrophages via ROS/PI3K signaling-dependent various cytokine production to promote NSCLC growth
    Authors: J Zhang, H Li, Q Wu, Y Chen, Y Deng, Z Yang, L Zhang, B Liu
    Redox Biol, 2019-02-06;22(0):101116.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  25. Intratumoral expression using a NFkB-based promoter enhances IL12 antitumor efficacy
    Authors: G Garaulet, G Pérez-Chac, H Alarcón, A Alfranca, F Mulero, J Martínez-T, JM Zapata, A Rodríguez
    Cancer Gene Ther., 2019-01-09;0(0):.
    Species: Mouse
    Sample Types: Serum
  26. Non-replicating Newcastle Disease Virus as an adjuvant for DNA vaccine enhances antitumor efficacy through the induction of TRAIL and granzyme B expression
    Authors: A Mohebbi, MS Ebrahimzad, S Baghban Ra, M Saeidi, A Tabarraei, SR Mohebbi, S Shirian, A Gorji, A Ghaemi
    Virus Res., 2018-12-29;261(0):72-80.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  27. Cutting Edge: Quantitative Determination of CD40L Threshold for IL-12 and IL-23 Production from Dendritic Cells
    Authors: K Abdi, K Laky, K Padhan, C Petrovas, J Skinner, J Kabat, DW Dorward, J Brzostowsk, EO Long, G Trinchieri, R Varma
    J. Immunol., 2018-10-12;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  28. ?-Synuclein disrupts the anti-inflammatory role of Drd2 via interfering ?-arrestin2-TAB1 interaction in astrocytes
    Authors: RH Du, Y Zhou, ML Xia, M Lu, JH Ding, G Hu
    J Neuroinflammation, 2018-09-10;15(1):258.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  29. Regulated intratumoral expression of IL-12 using a RheoSwitch Therapeutic System� (RTS�) gene switch as gene therapy for the treatment of glioma
    Authors: JA Barrett, H Cai, J Miao, PD Khare, P Gonzalez, J Dalsing-He, G Sharma, T Chan, LJN Cooper, F Lebel
    Cancer Gene Ther., 2018-05-14;0(0):.
    Species: Mouse
    Sample Types: Serum
  30. Carnosic acid protects mice from high-fat diet-induced NAFLD by regulating MARCKS
    Authors: HM Song, X Li, YY Liu, WP Lu, ZH Cui, L Zhou, D Yao, HM Zhang
    Int. J. Mol. Med., 2018-03-27;0(0):.
    Species: Mouse
    Sample Types: Serum
  31. Topical use and systemic action of green and roasted coffee oils and ground oils in a cutaneous incision model in rats (Rattus norvegicus albinus)
    Authors: BG Lania, J Morari, AL Souza, MND Silva, AR de Almeida, G Veira-Dami, SM Alegre, CL César, LA Velloso, ML Cintra, NB Maia, PENF Velho
    PLoS ONE, 2017-12-13;12(12):e0188779.
    Species: Rat
    Sample Types: Serum
  32. Fungal immunomodulatory protein-fve could modulate airway remodel through by affect IL17 cytokine
    Authors: YT Lee, CT Wu, HL Sun, JL Ko, KH Lue
    J Microbiol Immunol Infect, 2017-06-29;0(0):.
    Species: Mouse
    Sample Types: BALF
  33. STAT1 Represses Cytokine-Producing Group 2 and Group 3 Innate Lymphoid Cells during Viral Infection
    Authors: MT Stier, K Goleniewsk, JY Cephus, DC Newcomb, TP Sherrill, KL Boyd, MH Bloodworth, ML Moore, K Chen, JK Kolls, RS Peebles
    J. Immunol., 2017-06-02;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  34. Gene Electrotransfer of Plasmid-Encoding IL-12 Recruits the M1 Macrophages and Antigen-Presenting Cells Inducing the Eradication of Aggressive B16F10 Murine Melanoma
    Authors: U Lampreht T, L Loiacono, M Cemazar, U Kamensek, VM Fazio, G Sersa, E Signori
    Mediators Inflamm., 2017-05-16;2017(0):5285890.
    Species: Mouse
    Sample Types: Serum
  35. Efficacy of Juzentaihoto for Tumor Immunotherapy in B16 Melanoma Metastasis Model
    Authors: S Ishikawa, T Ishikawa, C Tezuka, K Asano, M Sunagawa, T Hisamitsu
    Evid Based Complement Alternat Med, 2017-02-12;2017(0):6054706.
    Species: Mouse
    Sample Types: Serum
  36. Microwave ablation combined with OK-432 induces Th1-type response and specific antitumor immunity in a murine model of breast cancer
    Authors: L Li, W Wang, H Pan, G Ma, X Shi, H Xie, X Liu, Q Ding, W Zhou, S Wang
    J Transl Med, 2017-01-31;15(1):23.
    Species: Mouse
    Sample Types: Plasma
  37. Tailor-made fibroblast-specific and antibiotic-free interleukin 12 plasmid for gene electrotransfer-mediated cancer immunotherapy
    Authors: Maja Cemazar
    Plasmid, 2016-11-24;89(0):9-15.
    Species: Human, Mouse
    Sample Types: Cell Culture Supernates
  38. Subtilisin-like protease-1 secreted through type IV secretion system contributes to high virulence of Streptococcus suis 2
    Authors: Supeng Yin
    Sci Rep, 2016-06-08;6(0):27369.
    Species: Mouse
    Sample Types: Serum
  39. Epigenetic changes in bone marrow progenitor cells influence the inflammatory phenotype and alter wound healing in type 2 diabetes.
    Authors: Gallagher K, Joshi A, Carson W, Schaller M, Allen R, Mukerjee S, Kittan N, Feldman E, Henke P, Hogaboam C, Burant C, Kunkel S
    Diabetes, 2014-11-03;64(4):1420-30.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  40. Prostaglandin E2 negatively regulates the production of inflammatory cytokines/chemokines and IL-17 in visceral leishmaniasis.
    Authors: Saha A, Biswas A, Srivastav S, Mukherjee M, Das P, Ukil A
    J Immunol, 2014-07-21;193(5):2330-9.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  41. The effects of protein malnutrition on the TNF-RI and NF-kappaB expression via the TNF-alpha signaling pathway.
    Authors: de Oliveira D, Hastreiter A, Mello A, de Oliveira Beltran J, Oliveira Santos E, Borelli P, Fock R
    Cytokine, 2014-07-06;69(2):218-25.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  42. Leishmania donovani targets tumor necrosis factor receptor-associated factor (TRAF) 3 for impairing TLR4-mediated host response.
    Authors: Gupta P, Giri J, Srivastav S, Chande A, Mukhopadhyaya R, Das P, Ukil A
    FASEB J, 2014-01-03;28(4):1756-68.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  43. Evaluation of p21 promoter for interleukin 12 radiation induced transcriptional targeting in a mouse tumor model.
    Authors: Kamensek, Urska, Sersa, Gregor, Cemazar, Maja
    Mol Cancer, 2013-11-12;12(1):136.
    Species: Mouse
    Sample Types: Tissue Homogenates
  44. Hypomorphic mutation in the RAG2 gene affects dendritic cell distribution and migration.
    Authors: Maina, Virginia, Marrella, Veronica, Mantero, Stefano, Cassani, Barbara, Fontana, Elena, Anselmo, Achille, Del Prete, Annalisa, Sozzani, Silvano, Vezzoni, Paolo, Poliani, Pietro L, Villa, Anna
    J Leukoc Biol, 2013-09-19;94(6):1221-30.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  45. PGI(2) signaling inhibits antigen uptake and increases migration of immature dendritic cells.
    Authors: Toki S, Goleniewska K, Huckabee M, Zhou W, Newcomb D, FitzGerald G, Lawson W, Peebles R
    J Leukoc Biol, 2013-04-26;94(1):77-88.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  46. Enhanced immunogenicity of a tricomponent mannan tetanus toxoid conjugate vaccine targeted to dendritic cells via Dectin-1 by incorporating beta-glucan.
    Authors: Lipinski T, Fitieh A, St Pierre J, Ostergaard H, Bundle D, Touret N
    J Immunol, 2013-03-20;190(8):4116-28.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  47. Antisense oligonucleotide treatment enhances the recovery of acute lung injury through IL-10-secreting M2-like macrophage-induced expansion of CD4+ regulatory T cells.
    Authors: Guo Z, Wen Z, Qin A, Zhou Y, Liao Z, Liu Z, Liang Y, Ren T, Xu L
    J Immunol, 2013-03-20;190(8):4337-48.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  48. TLR-mediated distinct IFN-gamma/IL-10 pattern induces protective immunity against murine visceral leishmaniasis.
    Eur. J. Immunol., 2012-08-01;42(8):2087-99.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  49. IL-10-Producing Th1 Cells and Disease Progression Are Regulated by Distinct CD11c+ Cell Populations during Visceral Leishmaniasis.
    Authors: Owens BM, Beattie L, Moore JW, Brown N, Mann JL, Dalton JE, Maroof A, Kaye PM
    PLoS Pathog., 2012-07-26;8(7):e1002827.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  50. The Receptor Slamf1 on the Surface of Myeloid Lineage Cells Controls Susceptibility to Infection by Trypanosoma cruzi.
    Authors: Calderon J, Maganto-Garcia E, Punzon C, Carrion J, Terhorst C, Fresno M
    PLoS Pathog., 2012-07-12;8(7):e1002799.
    Species: Mouse
    Sample Types: Serum
  51. Protein kinase C delta (PKCdelta)-extracellular signal-regulated kinase 1/2 (ERK1/2) signaling cascade regulates glycogen synthase kinase-3 (GSK-3) inhibition-mediated interleukin-10 (IL-10) expression in lipopolysaccharide (LPS)-induced endotoxemia.
    Authors: Noh KT, Son KH, Jung ID, Kang HK, Hwang SA, Lee WS, You JC, Park YM
    J. Biol. Chem., 2012-03-05;287(17):14226-33.
    Species: Mouse
    Sample Types: Serum
  52. The interaction between IL-18 and IL-18 receptor limits the magnitude of protective immunity and enhances pathogenic responses following infection with intracellular bacteria.
    Authors: Ghose P, Ali AQ, Fang R, Forbes D, Ballard B, Ismail N
    J. Immunol., 2011-06-29;187(3):1333-46.
    Species: Mouse
    Sample Types: Serum
  53. Improved host defense against septic peritonitis in mice lacking MyD88 and TRIF is linked to a normal interferon response.
    Authors: Reim D, Rossmann-Bloeck T, Jusek G, Prazeres da Costa O, Holzmann B
    J. Leukoc. Biol., 2011-05-31;90(3):613-20.
    Species: Mouse
    Sample Types: Plasma
  54. Dual functions of prostaglandin D2 in murine contact hypersensitivity via DP and CRTH2.
    Authors: Yamamoto Y, Otani S, Hirai H, Nagata K, Aritake K, Urade Y, Narumiya S, Yokozeki H, Nakamura M, Satoh T
    Am. J. Pathol., 2011-05-10;179(1):302-14.
    Species: Mouse
    Sample Types: Tissue Homogenates
  55. Tumors Suppress In Situ Proliferation of Cytotoxic T Cells by Promoting Differentiation of Gr-1+ Conventional Dendritic Cells through IL-6.
    Authors: Diao J, Zhao J, Winter E
    J. Immunol., 2011-03-23;186(9):5058-67.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  56. The TLR7 ligand 9-benzyl-2-butoxy-8-hydroxy adenine inhibits IL-17 response by eliciting IL-10 and IL-10-inducing cytokines.
    Authors: Vultaggio A, Nencini F, Pratesi S, Maggi L, Guarna A, Annunziato F, Romagnani S, Parronchi P, Maggi E
    J. Immunol., 2011-03-09;186(8):4707-15.
    Species: Mouse
    Sample Types: Serum
  57. Histone Deacetylase Inhibitor LAQ824 Augments Inflammatory Responses in Macrophages through Transcriptional Regulation of IL-10.
    Authors: Wang H, Cheng F
    J. Immunol., 2011-03-02;186(0):3986.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  58. Anti-inflammatory effects of FTY720 do not prevent neuronal cell loss in a rat model of optic neuritis.
    Authors: Rau CR, Hein K, Sattler MB, Kretzschmar B, Hillgruber C, McRae BL, Diem R, Bahr M
    Am. J. Pathol., 2011-02-26;178(4):1770-81.
    Species: Rat
    Sample Types: Tissue Homogenates
  59. Zoledronic acid impairs myeloid differentiation to tumour-associated macrophages in mesothelioma.
    Authors: Veltman JD, Lambers ME, van Nimwegen M
    Br. J. Cancer, 2010-07-27;103(5):629-41.
    Species: Mouse
    Sample Types: Peritoneal Fluid
  60. Proinflammatory clearance of apoptotic neutrophils induces an IL-12(low)IL-10(high) regulatory phenotype in macrophages.
    Authors: Filardy AA, Pires DR, Nunes MP, Takiya CM, Freire-de-Lima CG, Ribeiro-Gomes FL, DosReis GA
    J. Immunol., 2010-07-21;185(4):2044-50.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  61. Critical role of the disintegrin metalloprotease ADAM17 for intestinal inflammation and regeneration in mice.
    Authors: Chalaris A, Adam N, Sina C, Rosenstiel P, Lehmann-Koch J, Schirmacher P, Hartmann D, Cichy J, Gavrilova O, Schreiber S, Jostock T, Matthews V, Hasler R, Becker C, Neurath MF, Reiss K, Saftig P, Scheller J, Rose-John S
    J. Exp. Med., 2010-07-05;207(8):1617-24.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  62. Oncostatin M induces dendritic cell maturation and Th1 polarization.
    Authors: Jung ID, Noh KT, Lee CM, Chun SH, Jeong SK, Park JW, Park WS, Kim HW, Yun CH, Shin YK, Park YM
    Biochem. Biophys. Res. Commun., 2010-03-04;394(2):272-8.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  63. Varying susceptibility of pulmonary cytokine production to lipopolysaccharide in mice.
    Authors: Alm AS, Li K, Yang D, Andersson R, Lu Y, Wang X
    Cytokine, 2009-12-29;49(3):256-63.
    Species: Mouse
    Sample Types: BALF
  64. The CCL7-CCL2-CCR2 axis regulates IL-4 production in lungs and fungal immunity.
    Authors: Szymczak WA, Deepe GS
    J. Immunol., 2009-07-08;183(3):1964-74.
    Species: Mouse
    Sample Types: Tissue Homogenates
  65. TLR9 activation coupled to IL-10 deficiency induces adverse pregnancy outcomes.
    Authors: Thaxton JE, Romero R, Sharma S
    J. Immunol., 2009-06-26;183(2):1144-54.
    Species: Mouse
    Sample Types: Serum
  66. A MyD88-dependent early IL-17 production protects mice against airway infection with the obligate intracellular pathogen Chlamydia muridarum.
    Authors: Zhang X, Gao L, Lei L, Zhong Y, Dube P, Berton MT, Arulanandam B, Zhang J, Zhong G
    J. Immunol., 2009-06-19;183(2):1291-300.
    Species: Mouse
    Sample Types: BALF
  67. TLR9 agonist, but not TLR7/8, functions as an adjuvant to diminish FI-RSV vaccine-enhanced disease, while either agonist used as therapy during primary RSV infection increases disease severity.
    Authors: Johnson TR, Rao S, Seder RA, Chen M, Graham BS
    Vaccine, 2009-04-03;27(23):3045-52.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  68. Intravenous tolerance modulates macrophage classical activation and antigen presentation in experimental autoimmune encephalomyelitis.
    Authors: Li H, Ciric B, Yang J, Xu H, Fitzgerald DC, Elbehi M, Fonseca-Kelly Z, Yu S, Zhang GX, Rostami A
    J. Neuroimmunol., 2009-02-01;208(1):54-60.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  69. Inhibition of monocyte-derived inflammatory cytokines by IL-25 occurs via p38 Map kinase-dependent induction of Socs-3.
    Authors: Caruso R, Stolfi C, Sarra M, Rizzo A, Fantini MC, Pallone F, MacDonald TT, Monteleone G
    Blood, 2009-01-07;113(15):3512-9.
    Species: Mouse
    Sample Types: Serum
  70. Pulmonary stromal cells induce the generation of regulatory DC attenuating T-cell-mediated lung inflammation.
    Authors: Li Q, Guo Z, Xu X, Xia S, Cao X
    Eur. J. Immunol., 2008-10-01;38(10):2751-61.
    Species: Mouse
    Sample Types: BALF
  71. Essential involvement of CX3CR1-mediated signals in the bactericidal host defense during septic peritonitis.
    Authors: Ishida Y, Hayashi T, Goto T, Kimura A, Akimoto S, Mukaida N, Kondo T
    J. Immunol., 2008-09-15;181(6):4208-18.
    Species: Mouse
    Sample Types: Peritoneal Lavage Fluid
  72. TLR-2 and IL-17A in chitin-induced macrophage activation and acute inflammation.
    Authors: Da Silva CA, Hartl D, Liu W, Lee CG, Elias JA
    J. Immunol., 2008-09-15;181(6):4279-86.
    Species: Mouse
    Sample Types: BALF
  73. Combined triggering of dendritic cell receptors results in synergistic activation and potent cytotoxic immunity.
    Authors: Wells JW, Cowled CJ, Farzaneh F
    J. Immunol., 2008-09-01;181(5):3422-31.
    Species: Mouse
    Sample Types: Serum
  74. Curcumin attenuates ovalbumin-induced airway inflammation by regulating nitric oxide.
    Authors: Moon DO, Kim MO, Lee HJ, Choi YH, Park YM, Heo MS, Kim GY
    Biochem. Biophys. Res. Commun., 2008-08-13;375(2):275-9.
    Species: Mouse
    Sample Types: BALF
  75. The function of TRADD in signaling through tumor necrosis factor receptor 1 and TRIF-dependent Toll-like receptors.
    Authors: Pobezinskaya YL, Kim YS, Choksi S, Morgan MJ, Li T, Liu C, Liu Z
    Nat. Immunol., 2008-07-20;9(9):1047-54.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  76. The novel inosine analogue, INO-2002, protects against diabetes development in multiple low-dose streptozotocin and non-obese diabetic mouse models of type I diabetes.
    Authors: Mabley JG, Pacher P, Murthy KG, Williams W, Southan GJ, Salzman AL, Szabo C
    J. Endocrinol., 2008-06-18;198(3):581-9.
    Species: Mouse
    Sample Types: Tissue Homogenates
  77. Antifibrotic action of pirfenidone and prednisolone: different effects on pulmonary cytokines and growth factors in bleomycin-induced murine pulmonary fibrosis.
    Authors: Oku H, Shimizu T, Kawabata T, Nagira M, Hikita I, Ueyama A, Matsushima S, Torii M, Arimura A
    Eur. J. Pharmacol., 2008-06-16;590(1):400-8.
    Species: Mouse
    Sample Types: Tissue Homogenates
  78. CD47 deficiency protects mice from lipopolysaccharide-induced acute lung injury and Escherichia coli pneumonia.
    Authors: Su X, Johansen M, Looney MR, Brown EJ, Matthay MA
    J. Immunol., 2008-05-15;180(10):6947-53.
    Species: Mouse
    Sample Types: BALF
  79. Dendritic cells derived from TBP-2-deficient mice are defective in inducing T cell responses.
    Authors: Son A, Nakamura H, Okuyama H, Oka S, Yoshihara E, Liu W, Matsuo Y, Kondo N, Masutani H, Ishii Y, Iyoda T, Inaba K, Yodoi J
    Eur. J. Immunol., 2008-05-01;38(5):1358-67.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  80. Morphine withdrawal inhibits IL-12 induction in a macrophage cell line through a mechanism that involves cAMP.
    Authors: Kelschenbach J, Ninkovic J, Wang J, Krishnan A, Charboneau R, Barke RA, Roy S
    J. Immunol., 2008-03-15;180(6):3670-9.
    Species: Mouse
    Sample Types: Whole Cells
  81. A tumor-selective biotherapy with prolonged impact on established metastases based on cytokine gene-engineered MSCs.
    Authors: Chen X, Lin X, Zhao J, Shi W, Zhang H, Wang Y, Kan B, DU L, Wang B, Wei Y, Liu Y, Zhao X
    Mol. Ther., 2008-02-05;16(4):749-56.
    Species: Mouse
    Sample Types: Serum
  82. CD40 and CD80/86 act synergistically to regulate inflammation and mortality in polymicrobial sepsis.
    Authors: Nolan A, Weiden M, Kelly A, Hoshino Y, Hoshino S, Mehta N, Gold JA
    Am. J. Respir. Crit. Care Med., 2007-11-07;177(3):301-8.
    Species: Mouse
    Sample Types: Plasma
  83. Reversion of immune tolerance in advanced malignancy: modulation of myeloid-derived suppressor cell development by blockade of stem-cell factor function.
    Authors: Pan PY, Wang GX, Yin B, Ozao J, Ku T, Divino CM, Chen SH
    Blood, 2007-09-20;111(1):219-28.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  84. Granulocyte-macrophage colony-stimulating factor prevents diabetes development in NOD mice by inducing tolerogenic dendritic cells that sustain the suppressive function of CD4+CD25+ regulatory T cells.
    Authors: Gaudreau S, Guindi C, Menard M, Besin G, Dupuis G, Amrani A
    J. Immunol., 2007-09-15;179(6):3638-47.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  85. Dectin-1 interaction with Mycobacterium tuberculosis leads to enhanced IL-12p40 production by splenic dendritic cells.
    Authors: Rothfuchs AG, Bafica A, Feng CG, Egen JG, Williams DL, Brown GD, Sher A
    J. Immunol., 2007-09-15;179(6):3463-71.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  86. Mrp8 and Mrp14 are endogenous activators of Toll-like receptor 4, promoting lethal, endotoxin-induced shock.
    Authors: Vogl T, Tenbrock K, Ludwig S, Leukert N, Ehrhardt C, van Zoelen MA, Nacken W, Foell D, van der Poll T, Sorg C, Roth J
    Nat. Med., 2007-09-02;13(9):1042-9.
    Species: Mouse
    Sample Types: Serum
  87. Placental growth factor down-regulates type 1 T helper immune response by modulating the function of dendritic cells.
    Authors: Lin YL, Liang YC, Chiang BL
    J. Leukoc. Biol., 2007-08-30;82(6):1473-80.
    Species: Human
    Sample Types: Cell Culture Supernates
  88. IL-27 synthesis induced by TLR ligation critically depends on IFN regulatory factor 3.
    Authors: Molle C, Nguyen M, Flamand V, Renneson J, Trottein F, De Wit D, Willems F, Goldman M, Goriely S
    J. Immunol., 2007-06-15;178(12):7607-15.
    Species: Mouse
    Sample Types: Serum
  89. The direct action of 1alpha,25(OH)2-vitamin D3 on purified mouse Langerhans cells.
    Authors: Fujita H, Asahina A, Komine M, Tamaki K
    Cell. Immunol., 2007-05-15;245(2):70-9.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  90. Therapeutic targeting of CC ligand 21 or CC chemokine receptor 7 abrogates pulmonary fibrosis induced by the adoptive transfer of human pulmonary fibroblasts to immunodeficient mice.
    Authors: Pierce EM, Carpenter K, Jakubzick C, Kunkel SL, Flaherty KR, Martinez FJ, Hogaboam CM
    Am. J. Pathol., 2007-04-01;170(4):1152-64.
    Species: Mouse
    Sample Types: Tissue Homogenates
  91. The immunomodulation of endotoxin-induced acute lung injury by hesperidin in vivo and in vitro.
    Authors: Yeh CC, Kao SJ, Lin CC, Wang SD, Liu CJ, Kao ST
    Life Sci., 2007-02-03;80(20):1821-31.
    Species: Mouse
    Sample Types: BALF
  92. Protectin D1 is generated in asthma and dampens airway inflammation and hyperresponsiveness.
    Authors: Levy BD, Kohli P, Gotlinger K, Haworth O, Hong S, Kazani S, Israel E, Haley KJ, Serhan CN
    J. Immunol., 2007-01-01;178(1):496-502.
    Species: Mouse
    Sample Types: BALF
  93. CD27 contributes to the early systemic immune response to Mycobacterium tuberculosis infection but does not affect outcome.
    Authors: Wieland CW, Kerver ME, Florquin S, Nolte MA, Borst J, van Lier R, van Oers MH, van der Poll T
    Int. Immunol., 2006-09-11;18(11):1531-9.
    Species: Mouse
    Sample Types: Tissue Homogenates
  94. Adenosine A2A receptor inactivation increases survival in polymicrobial sepsis.
    Authors: Nemeth ZH, Csoka B, Wilmanski J, Xu D, Lu Q, Ledent C, Deitch EA, Pacher P, Spolarics Z, Hasko G
    J. Immunol., 2006-05-01;176(9):5616-26.
    Species: Mouse
    Sample Types: Plasma
  95. Inhalation of ultrafine carbon particles triggers biphasic pro-inflammatory response in the mouse lung.
    Authors: Andre E, Stoeger T, Takenaka S, Bahnweg M, Ritter B, Karg E, Lentner B, Reinhard C, Schulz H, Wjst M
    Eur. Respir. J., 2006-04-26;28(2):275-85.
    Species: Mouse
    Sample Types: BALF
  96. MyD88-dependent and -independent murine cytomegalovirus sensing for IFN-alpha release and initiation of immune responses in vivo.
    Authors: Delale T, Paquin A, Asselin-Paturel C, Dalod M, Brizard G, Bates EE, Kastner P, Chan S, Akira S, Vicari A, Biron CA, Trinchieri G, Briere F
    J. Immunol., 2005-11-15;175(10):6723-32.
    Species: Mouse
    Sample Types: Serum
  97. Immunization with gp120-depleted whole killed HIV immunogen and a second-generation CpG DNA elicits strong HIV-specific responses in mice.
    Authors: Trabattoni D, Clivio A, Bray DH, Bhagat L, Beltrami S, Maffeis G, Cesana E, Lowry P, Lissoni F, Kandimalla ER, Sullivan T, Agrawal S, Bartholomew R, Clerici M
    Vaccine, 2005-09-22;24(9):1470-7.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  98. Suppression of autoimmune retinal disease by lovastatin does not require Th2 cytokine induction.
    Authors: Gegg ME, Harry R, Hankey D, Zambarakji H, Pryce G, Baker D, Adamson P, Calder V, Greenwood J
    J. Immunol., 2005-02-15;174(4):2327-35.
    Species: Rat
    Sample Types: Cell Culture Supernates
  99. Endogenous interleukin-12 improves the early antimicrobial host response to murine Escherichia coli peritonitis.
    Authors: Weijer S, Florquin S, van der Poll T
    Shock, 2005-01-01;23(1):54-8.
    Species: Mouse
    Sample Types: Peritoneal Fluid
  100. Vaccination of apoptotic Fas ligand-expressing tumors decreased antitumor responses by enhanced production of immunosuppressive cytokines.
    Authors: Wada A, Tada Y, Shimozato O, Takiguchi Y, Tatsumi K, Kuriyama T, Tagawa M
    Anticancer Res., 2005-01-01;25(1):299-303.
    Species: Mouse
    Sample Types: Tissue Secretion
  101. Gamma interferon does not enhance clearance of Pseudomonas aeruginosa but does amplify a proinflammatory response in a murine model of postseptic immunosuppression.
    Authors: Murphey ED, Herndon DN, Sherwood ER
    Infect. Immun., 2004-12-01;72(12):6892-901.
    Species: Mouse
    Sample Types: Plasma
  102. Increased interleukin-10 associated with low IL-6 concentration correlated with greater survival rates in mice infected by rabies virus vaccinated against it and immunomodulated with P. acnes.
    Authors: Megid J, Kaneno R, Nozaki CN, Brito CJ, Almeida MF
    Comp. Immunol. Microbiol. Infect. Dis., 2004-11-01;27(6):393-411.
    Species: Mouse
    Sample Types: Serum
  103. Increased and long-term generation of dendritic cells with reduced function from IL-6-deficient bone marrow.
    Authors: Bleier JI, Pillarisetty VG, Shah AB, DeMatteo RP
    J. Immunol., 2004-06-15;172(12):7408-16.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  104. Effects of poly(ADP-ribose) polymerase inhibition on inflammatory cell migration in a murine model of asthma.
    Authors: Virag L, Bai P, Bak I, Pacher P, Mabley JG, Liaudet L, Bakondi E, Gergely P, Kollai M, Szabo C
    Med. Sci. Monit., 2004-03-01;10(3):BR77-83.
    Species: Mouse
    Sample Types: BALF
  105. Distinct modulatory effects of LPS and CpG on IL-18-dependent IFN-gamma synthesis.
    Authors: Gould MP, Greene JA, Bhoj V, DeVecchio JL, Heinzel FP
    J. Immunol., 2004-02-01;172(3):1754-62.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  106. A protective role of locally administered immunostimulatory CpG oligodeoxynucleotide in a mouse model of genital herpes infection.
    Authors: Harandi AM, Eriksson K, Holmgren J
    J. Virol., 2003-01-01;77(2):953-62.
    Species: Mouse
    Sample Types: Serum
  107. Endogenous granulocyte-macrophage colony-stimulating factor overexpression in vivo results in the long-term recruitment of a distinct dendritic cell population with enhanced immunostimulatory function.
    Authors: Miller G, 107331, Pillarisetty VG, Shah AB, Lahrs S, Xing Z, DeMatteo RP
    Lamin A/C impairments cause mitochondrial dysfunction by attenuating PGC1alpha and the NAMPT-NAD+ pathway, 2002-09-15;169(6):2875-85.
    Species: Mouse
    Sample Types: Serum
  108. Cutting edge: myeloid differentiation factor 88 deficiency improves resistance against sepsis caused by polymicrobial infection.
    Authors: Weighardt H, Bê Ë„Æ%í, Kaiser-Moore S, Vabulas RM, Kirschning CJ, Wagner H, Holzmann B
    2002-09-15;169(6):2823-7.
    Species: Mouse
    Sample Types: Serum
  109. A profibrotic function of IL-12p40 in experimental pulmonary fibrosis.
    Authors: Huaux F, 107322, Arras M, Tomasi D, Barbarin V, Delos M, Coutelier JP, Vink A, Phan SH, Renauld JC, Lison D
    Interferon-gamma modulates articular chondrocyte and osteoblast metabolism through protein kinase R-independent and dependent mechanisms, 2002-09-01;169(5):2653-61.
    Species: Mouse
    Sample Types: Tissue Homogenates
  110. The protein tyrosine kinase inhibitor AG126 prevents the massive microglial cytokine induction by pneumococcal cell walls.
    Authors: Hanisch UK, Prinz M, Angstwurm K, Hausler KG, Kann O, Kettenmann H, Weber JR
    Eur. J. Immunol., 2001-07-01;31(7):2104-15.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  111. Local anticandidal immune responses in a rat model of vaginal infection by and protection against Candida albicans.
    Authors: de Bernardis F, Santoni G, Boccanera M, Spreghini E, Adriani D, Morelli L, Cassone A
    Infect. Immun., 2000-06-01;68(6):3297-304.
    Species: Rat
    Sample Types: Vaginal Fluid
  112. In vitro and in vivo immunostimulatory potential of bone marrow-derived mast cells on B- and T-lymphocyte activation.
    Authors: Tkaczyk C, Villa I, Peronet R, David B, Chouaib S, Mecheri S
    J. Allergy Clin. Immunol., 2000-01-01;105(1):134-42.
    Species: Mouse
    Sample Types: Cell Culture Supernates

FAQs

  1. Assay Diluent RD1-14 contains visible precipitate. Is this acceptable to use?

    • A small amount of precipitate is expected and acceptable in Assay Diluent RD1-14. Variability has been observed between vials and diluent lots. It may not fully resolubilize but this will not impact assay performance. It is not recommended to filter the precipitate.

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Mouse IL-12 p70 Quantikine ELISA Kit
By Anonymous on 11/02/2018
Sample Tested: Serum
Mouse IL-12 p70 Quantikine ELISA Kit
By Anonymous on 10/07/2018
Sample Tested: EDTA Plasma
Mouse IL-12 p70 Quantikine ELISA Kit M1270