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Mouse IL-15R alpha Antibody

Catalog #: AF551
7 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF551
AF551-SP
Cell Proliferation Induced by IL‑15 and Neutralization by Mouse IL‑15 R alpha Antibody.
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Citations (7)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IL-15R alpha Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-15 R alpha in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) IL‑15 R alpha is observed and less than 1% cross-reactivity with rhIL-2 R alpha, recombinant mouse (rm) IL-2 R beta, and rmIL-2 R gamma is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IL-15 R alpha
Gly33-Lys205
Accession # Q60819
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Purity
Immunogen affinity purified

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse IL‑15 R alpha Fc Chimera (Catalog # 551-MR)
Flow Cytometry
2.5 µg/106 cells
EL‑4 mouse lymphoblast cell line
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Neutralization
Measured by its ability to neutralize IL‑15-induced proliferation in the CTLL‑2 mouse cytotoxic T cell line. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 276. The Neutralization Dose (ND50) is typically 0.3-1.5 µg/mL in the presence of 0.8 ng/mL Recombinant Human IL‑15.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by IL‑15 and Neutralization by Mouse IL‑15 R alpha Antibody. View Larger

Cell Proliferation Induced by IL‑15 and Neutralization by Mouse IL‑15 R alpha Antibody. Recombinant Human IL‑15 (Catalog # 247-IL) stimulates proliferation in the CTLL‑2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑15 (0.8 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL‑15 Ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF551). The ND50 is typically 0.3-1.5 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-15R alpha

Interleukin 15 receptor alpha (IL-15 R alpha ) is a high affinity receptor that specifically binds IL-15 with high affinity and associates as a heterotrimer with the IL-2 receptors beta and gamma subunits to initiate signal transduction. IL-15 R alpha is expressed on a wide variety of T cells and B cells as well as non-lymphoid cells. IL‑15 R alpha is a 58-60 kDa protein that shares structural similarities to the IL-2 R alpha protein. IL-15 R alpha and IL-2 R alpha genes also share similar intron-exon organization and are closely linked on human chromosome 10p14-p15. Human IL-15 R alpha shares 45% amino acid (aa) homology with the mouse form of the receptor. Signaling of IL-15 can occur in one of three ways; through the heterotrimeric complex of IL-15 R alpha, IL-2 R beta, and IL-2 R gamma c, through the heterodimeric complex of IL-2 receptors beta and gamma common, through a novel 60-65 kDa IL-15 RX subunit found on mast cells. The binding of IL-15 to IL-15 R alpha has been reported to antagonize the TNF-alpha -mediated apoptosis in fibroblasts by competing with TNF RI for TRAF2 binding.

References
  1. Anderson, D.M. et al. (1995) J. Biol. Chem. 270:29862.
  2. Bulfone-Paus, S. et al. (1999) FASEB 13:1575.
  3. Waldemann, T.A. and Y. Tagaya (1999) Ann. Rev. Immunol. 17:19.
  4. Dubois, S. et al. (1999) J. Biol. Chem. 274:26978.
Long Name
Interleukin 15 Receptor alpha
Entrez Gene IDs
3601 (Human); 16169 (Mouse); 102121571 (Cynomolgus Monkey)
Alternate Names
CD215 antigen; CD215; IL15 R alpha; IL-15 R alpha; IL-15 receptor subunit alpha; IL-15R alpha; IL15RA; IL-15Ra; IL-15R-alpha; interleukin 15 receptor, alpha; interleukin-15 receptor subunit alpha; MGC104179

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Citations for Mouse IL-15R alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Anti-inflammatory cytokines directly inhibit innate but not adaptive CD8+ T cell functions.
    Authors: Freeman B, Meyer C, Slifka M
    J Virol, 2014-04-16;88(13):7474-84.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Paracrine and transpresentation functions of IL-15 are mediated by diverse splice versions of IL-15Ralpha in human monocytes and dendritic cells.
    Authors: Muller J, Waldmann T, Kruhlak M, Dubois S
    J Biol Chem, 2012-10-16;287(48):40328-38.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Mechanisms of NK cell-macrophage Bacillus anthracis crosstalk: a balance between stimulation by spores and differential disruption by toxins.
    Authors: Klezovich-Benard M, Corre JP, Jusforgues-Saklani H, Fiole D, Burjek N, Tournier JN, Goossens PL
    PLoS Pathog., 2012-01-12;8(1):e1002481.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  4. Immunological mechanisms and clinical implications of regulatory T cell deficiency in a systemic autoimmune disorder: roles of IL-2 versus IL-15.
    Authors: Yang CH, Tian L, Ling GS, Trendell-Smith NJ, Ma L, Lo CK, Stott DI, Liew FY, Huang FP
    Eur. J. Immunol., 2008-06-01;38(6):1664-76.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Mouse fetal and embryonic liver cells differentiate human umbilical cord blood progenitors into CD56-negative natural killer cell precursors in the absence of interleukin-15.
    Authors: McCullar V, Oostendorp R, Panoskaltsis-Mortari A, Yun G, Lutz CT, Wagner JE, Miller JS
    Exp. Hematol., 2008-03-04;36(5):598-608.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Combined stimulation with interleukin-18 and CpG induces murine natural killer dendritic cells to produce IFN-gamma and inhibit tumor growth.
    Authors: Chaudhry UI, Kingham TP, Plitas G, Katz SC, Raab JR, DeMatteo RP
    Cancer Res., 2006-11-01;66(21):10497-504.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Distinct modulatory effects of LPS and CpG on IL-18-dependent IFN-gamma synthesis.
    Authors: Gould MP, Greene JA, Bhoj V, DeVecchio JL, Heinzel FP
    J. Immunol., 2004-02-01;172(3):1754-62.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization

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