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Mouse IL-17/IL-17A Antibody

Catalog #: AF-421-NA
18 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF-421-NA
AF-421-SP
Detection of IL-17 in EL-4 Mouse Cell Line by Flow Cytometry.
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Product Details
Citations (18)
FAQs
Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IL-17/IL-17A Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse and rat IL-17 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human IL-17A is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse IL‑17
Thr22-Ala158
Accession # Q62386
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse IL‑17 (Catalog # 421-ML)
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
See below
Neutralization
Measured by its ability to neutralize IL‑17-induced IL‑6 secretion in the NIH‑3T3 mouse embryonic fibroblast cell line. Yao, Z. et al. (1995) Immunity 3:811. The Neutralization Dose (ND50) is typically 0.05-0.25 µg/mL in the presence of 10 ng/mL Recombinant Mouse IL‑17.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of IL-17 antibody in EL-4 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of IL-17 in EL-4 Mouse Cell Line by Flow Cytometry. EL-4 mouse lymphoblast cell line was treated for 16 hours with 50 ng/mL PMA then stained with Goat Anti-Mouse IL-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-421-NA, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Neutralization IL-6 Secretion Induced by IL-17 and Neutralization by Mouse IL-17 Antibody. View Larger

IL-6 Secretion Induced by IL-17 and Neutralization by Mouse IL-17 Antibody. Recombinant Mouse IL-17 (Catalog # 421-ML) stimulates IL-6 secretion in the NIH-3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line), as measured by the Mouse IL-6 Quantikine ELISA Kit (Catalog # M6000B). IL-6 secretion elicited by Recombinant Mouse IL-17 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-17 Antigen Affinity-purified Polyclonal Anti-body (Catalog # AF-421-NA). The ND50 is typically 0.05-0.25 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17/IL-17A

Interleukin 17 (also known as CTLA-8) is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpesvirus Saimiri. cDNA clones encoding IL-17 have been isolated from activated rat, mouse, and human T cells. Mouse IL-17 cDNA encodes a 158 amino acid (aa) residue precursor protein with a 21 amino acid residue signal peptide that is cleaved to yield the 137 aa residue mature  IL-17. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. At the amino acid level, mIL-17 shows 57% and 87% sequence identity with herpesvirus and rat IL-17, respectively. An IL-17 specific mouse cell surface receptor (IL-17 R) has been cloned. While the expression of IL-17 mRNA is restricted to activated alpha beta TCR+CD4-CD8-T cells, the expression of mIL-17 R mRNA has been detected in virtually all cells and tissues tested. IL-17 exhibits multiple biological activities on a variety of cells including: the induction of IL-6 and IL-8 production in fibroblasts; the enhancement of surface expression of ICAM-1 in fibroblasts; activation of NF-kappa B and costimulation of T cell proliferation.

References
  1. Kennedy, J. et al. (1996) J. Interferon Cytokine Res. 16:611.
  2. Yao, Z. et al. (1995) J. Immunol. 155:5483.
  3. Yao, Z. et al. (1995) Immunity 3:811.
  4. Rouvier, E. et al. (1993) J. Immunol. 150:5445.
Long Name
Interleukin 17
Entrez Gene IDs
3605 (Human); 16171 (Mouse); 301289 (Rat); 449530 (Porcine); 481837 (Canine); 102119976 (Cynomolgus Monkey)
Alternate Names
CTLA8; CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17; IL-17; IL17A; IL-17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; IL17interleukin-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A

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Citations for Mouse IL-17/IL-17A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

18 Citations: Showing 1 - 10
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  1. The parasitic worm product ES-62 promotes health- and life-span in a high calorie diet-accelerated mouse model of ageing
    Authors: J Crowe, FE Lumb, J Doonan, M Broussard, A Tarafdar, MA Pineda, C Landabaso, L Mulvey, PA Hoskisson, SA Babayan, C Selman, W Harnett, MM Harnett
    PLoS Pathog., 2020-03-12;16(3):e1008391.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Development of an IL-17A DNA Vaccine to Treat Systemic Lupus Erythematosus in Mice
    Authors: H Koriyama, Y Ikeda, H Nakagami, M Shimamura, S Yoshida, H Rakugi, R Morishita
    Vaccines (Basel), 2020-02-12;8(1):.
    Species: Mouse
    Sample Types: Protein
    Applications: Western Blot
  3. Targeting TMEM176B Enhances Antitumor Immunity and Augments the Efficacy of Immune Checkpoint Blockers by Unleashing Inflammasome Activation
    Authors: Mercedes Segovia, Sofia Russo, Mathias Jeldres, Yamil D. Mahmoud, Valentina Perez, Maite Duhalde et al.
    Cancer Cell
  4. QiShenYiQi pill attenuates atherosclerosis by promoting regulatory T cells, inhibiting T helper 17 cells and accelerating cholesterol excretion
    Authors: Li Peng, Chong-Shan Lv, Yun Zhao, Shao-Dong Chen, Yang Huang, Da-Wei Lu et al.
    Oncotarget
  5. IL-33 Signaling Regulates Innate IL-17A and IL-22 Production via Suppression of Prostaglandin E2 during Lung Fungal Infection
    Authors: JM Garth, KM Reeder, MS Godwin, JJ Mackel, CW Dunaway, JP Blackburn, C Steele
    J. Immunol., 2017-08-07;199(6):2140-2148.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  6. IL17 Promotes Mammary Tumor Progression by Changing the Behavior of Tumor Cells and Eliciting Tumorigenic Neutrophils Recruitment.
    Authors: Benevides L, da Fonseca D, Donate P, Tiezzi D, De Carvalho D, de Andrade J, Martins G, Silva J
    Cancer Res, 2015-07-24;75(18):3788-99.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  7. RETRACTED: Enhanced IL-17 signalling following myocardial ischaemia/reperfusion injury
    Authors: Seán P. Barry, Samir Ounzain, James McCormick, Tiziano M. Scarabelli, Carol Chen-Scarabelli, Louis I.I. Saravolatz et al.
    International Journal of Cardiology
  8. Deleterious Effect of the IL‐23/IL‐17A Axis and gamma δT Cells on Left Ventricular Remodeling After Myocardial Infarction
    Authors: Xiaoxiang Yan, Takashi Shichita, Yoshinori Katsumata, Tomohiro Matsuhashi, Hideyuki Ito, Kentaro Ito et al.
    Journal of the American Heart Association
  9. IL-17 and VEGF are necessary for efficient corneal nerve regeneration.
    Authors: Li Z, Burns AR, Han L, Rumbaut RE, Smith CW
    Am. J. Pathol., 2011-03-01;178(3):1106-16.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  10. Microenvironment-derived IL-1 and IL-17 interact in the control of lung metastasis.
    Authors: Carmi Y, Rinott G, Dotan S, Elkabets M, Rider P, Voronov E, Apte RN
    J. Immunol., 2011-02-07;186(6):3462-71.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  11. Targeting Tim-1 to overcome resistance to transplantation tolerance mediated by CD8 T17 cells.
    Authors: Yuan X, Ansari MJ, D'Addio F, Paez-Cortez J, Schmitt I, Donnarumma M, Boenisch O, Zhao X, Popoola J, Clarkson MR, Yagita H, Akiba H, Freeman GJ, Iacomini J, Turka LA, Glimcher LH, Sayegh MH
    Proc. Natl. Acad. Sci. U.S.A., 2009-06-15;106(26):10734-9.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  12. Prostaglandin mediates IL-23/IL-17-induced neutrophil migration in inflammation by inhibiting IL-12 and IFNgamma production.
    Authors: Lemos HP, Grespan R, Vieira SM, Cunha TM, Verri WA, Fernandes KS, Souto FO, McInnes IB, Ferreira SH, Liew FY, Cunha FQ
    Proc. Natl. Acad. Sci. U.S.A., 2009-03-16;106(14):5954-9.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  13. Anti-inflammatory effects of IL-17A on Helicobacter pylori-induced gastritis.
    Authors: Otani K, Watanabe T, Tanigawa T, Okazaki H, Yamagami H, Watanabe K, Tominaga K, Fujiwara Y, Oshitani N, Arakawa T
    Biochem. Biophys. Res. Commun., 2009-02-26;382(2):252-8.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  14. Tumor-specific Th17-polarized cells eradicate large established melanoma.
    Authors: Muranski P, Boni A, Antony PA, Cassard L, Irvine KR, Kaiser A, Paulos CM, Palmer DC, Touloukian CE, Ptak K, Gattinoni L, Wrzesinski C, Hinrichs CS, Kerstann KW, Feigenbaum L, Chan CC, Restifo NP
    Blood, 2008-03-19;112(2):362-73.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  15. Resveratrol inhibits high glucose-induced PI3K/Akt/ERK-dependent interleukin-17 expression in primary mouse cardiac fibroblasts.
    Authors: Venkatachalam K, Mummidi S, Cortez DM, Prabhu SD, Valente AJ, Chandrasekar B
    Am. J. Physiol. Heart Circ. Physiol., 2008-02-29;294(5):H2078-87.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  16. T-bet regulates the fate of Th1 and Th17 lymphocytes in autoimmunity.
    Authors: Gocke AR, Cravens PD, Ben LH, Hussain RZ, Northrop SC, Racke MK, Lovett-Racke AE
    J. Immunol., 2007-02-01;178(3):1341-8.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  17. Role of mesothelial cell-derived granulocyte colony-stimulating factor in interleukin-17-induced neutrophil accumulation in the peritoneum.
    Authors: Witowski J, Ksiazek K, Warnecke C, Kuzlan M, Korybalska K, Tayama H, Wisniewska-Elnur J, Pawlaczyk K, Trominska J, Breborowicz A, Jorres A
    Kidney Int., 2007-01-17;71(6):514-25.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  18. IL-17, produced by lymphocytes and neutrophils, is necessary for lipopolysaccharide-induced airway neutrophilia: IL-15 as a possible trigger.
    Authors: Ferretti S, Bonneau O, Dubois GR, Jones CE, Trifilieff A
    J. Immunol., 2003-02-15;170(4):2106-12.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization

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Mouse IL-17/IL-17A Antibody
By Anonymous on 11/27/2017
Application: ELISA Sample Tested: Serum and Plasma Species: Mouse

Antibody MAB421 was used as the capture with this antibody (AF421) as the detection in an ELISA.

ELISA Mouse IL-17/IL-17A Antibody AF-421-NA