Mouse IL-17E/IL-25 Antibody

Catalog #: MAB1399
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1399-SP
MAB1399-100
MAB1399-500

All IL-17E/IL-25 Antibodies

Product Details

Citations (3)

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IL-17E/IL-25 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IL-17E/IL-25 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant human (rh) IL‑17C, rhIL-17D, rhIL-17E, rhIL-17F, recombinant mouse (rm) IL-17, or rmIL-17B.

Source

Monoclonal Rat IgG_2B Clone # 207710

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse IL‑17E/IL-25
Val17-Ala169
Accession # NP_542767

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse IL-17E/IL-25 (Catalog # 1399-IL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17E/IL-25

The Interleukin 17 (IL-17) family proteins, comprising six members (IL-17, IL-17B through IL-17F), are secreted, structurally related proteins that share a conserved cysteine-knot fold near the C-terminus, but have considerable sequence divergence at the N-terminus. With the exception of IL-17B, which exists as a non-covalently linked dimer, all IL-17 family members are disulfide-linked dimers. IL-17 family proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions (1, 2). Mouse IL-17E cDNA encodes a 169 amino acid (aa) residues precursor protein with a putative 16 aa signal peptide (5). Mature mouse IL-17E shares 76% and 91% amino sequence (aa) identity with mature human and rat IL-17E, respectively. Mouse IL-17E also shares from 24% to 32% sequence identity with the other mouse IL‑17 family members. IL-17E expression was detected at very low levels by PCR in various peripheral tissues including brain, kidney, lung, prostate, testis, adrenal gland spinal cord, and trachea. IL-17E binds and activates IL-17 B Receptor (IL-17B R) (alternatively known as IL-17 Rh1, IL-17E R, and EVI27) (3, 4), which is expressed in kidney and liver, and at lower levels in brain, testis and other endocrine tissues. The expression of IL-17B R is up regulated under inflammatory conditions. Ligation of IL-17E to IL-17 RB induces activation of nuclear factor kappa-B and stimulates the production of the proinflamatory cytokine IL-8 (3). IL-17 has also been found to promote the expression of the prototypical Th2 genes (4, 5).

References

Aggarwal, S. and A.L. Gurney (2002) J. Leukoc. Biol. 71:1.
Moseley, T.A. et al. (2003) Cytokine & Growth Factor Rev. 14:155.
Lee, J. et al. (2001) J. Biol. Chem. 276:1660.
Hurst, S.D. et al. (2002) J. Immunol. 169:443.
Pan, G. et al. (2001) J. Immunol. 167:6569.

Long Name

Interleukin 17E

Entrez Gene IDs

64806 (Human); 140806 (Mouse); 501996 (Rat)

Alternate Names

IL17E; IL-17E; IL25; IL-25; interleukin 25; Interleukin-17E; interleukin-25

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse IL-17E/IL-25 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Epithelial cell-derived IL-25, but not Th17 cell-derived IL-17 or IL-17F, Is crucial for murine asthma.
Authors: Suzukawa M, Morita H, Nambu A, Arae K, Shimura E, Shibui A, Yamaguchi S, Suzukawa K, Nakanishi W, Oboki K, Kajiwara N, Ohno T, Ishii A, Korner H, Cua D, Suto H, Yoshimoto T, Iwakura Y, Yamasoba T, Ohta K, Sudo K, Saito H, Okumura K, Broide D, Matsumoto K, Nakae S
J Immunol, 2012-08-31;189(7):3641-52.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P
IL-25 enhances allergic airway inflammation by amplifying a TH2 cell-dependent pathway in mice.
Authors: Tamachi T, Maezawa Y, Ikeda K, Kagami S, Hatano M, Seto Y, Suto A, Suzuki K, Watanabe N, Saito Y, Tokuhisa T, Iwamoto I, Nakajima H
J. Allergy Clin. Immunol., 2006-06-21;118(3):606-14.
Species: Mouse
Sample Types: BALF
Applications: ELISA Development
Harnessing the Plasma Proteome to Mirror Current and Predict Future Cardiac Remodeling After Myocardial Infarction
Authors: Upendra Chalise, Mediha Becirovic-Agic, Jocelyn R. Rodriguez-Paar, Shelby R. Konfrst, Sharon D. B. de Morais, Catherine S. Johnson et al.
Journal of Cardiovascular Translational Research