Mouse IL-18 R alpha /IL-1 R5 Fluorescein-conjugated Antibody

Catalog #: FAB1216F
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB1216F-100
FAB1216F-025

Detection of IL‑18 R alpha /IL‑1 R5 in Mouse Splenocytes by Flow Cytometry.

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Citations (2)

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Mouse IL-18 R alpha /IL-1 R5 Fluorescein-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IL‑18 R alpha /IL‑1 R5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) IL-1 RI, rmIL-1 RII, recombinant human (rh) IL-1 R3, rhIL-1 R4, recombinant rat (rr) IL-1 R6, rmIL-1 R7, rhIL-1 R8, rhIL-1 R9, or rmSIGIRR is observed.

Source

Monoclonal Rat IgG₁ Clone # 112614

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse IL‑18 R alpha /IL‑1 R5
Ser19-Gly326
Accession # Q61098

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of IL‑18 R alpha /IL‑1 R5 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse IL-18 Ra/IL-1 R5 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1216F, filled histogram) or isotype control antibody (Catalog # IC005F, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-18 R alpha/IL-1 R5

IL-18 R, also known as IL-18 R alpha, is a member of the IL-1 receptor family and has been designated IL-1 R5.

Long Name

Interleukin 18 Receptor alpha

Entrez Gene IDs

8809 (Human); 16182 (Mouse)

Alternate Names

CD218a; CD218a;CDw218a;IL18R1;IL18RA;IL-18Ralpha;IL-18R-alpha;IL18Ralpha2;IL1RRP;IL-1Rrp;Interleukin-18 receptor 1; IL-1 R5; IL-1 Rrp; IL-18 R alpha; IL-18 R1; IL18R alpha; IL18R1; IL-18Ra

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Product Datasheet

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Related Research Areas

Citations for Mouse IL-18 R alpha /IL-1 R5 Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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IL-18 acts in synergy with IL-7 to promote ex vivo expansion of T lymphoid progenitor cells.
Authors: Gandhapudi S, Tan C, Marino J, Taylor A, Pack C, Gaikwad J, Van De Wiele C, Wren J, Teague T
J Immunol, 2015-03-16;194(8):3820-8.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Anti-inflammatory cytokines directly inhibit innate but not adaptive CD8+ T cell functions.
Authors: Freeman B, Meyer C, Slifka M
J Virol, 2014-04-16;88(13):7474-84.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry