Mouse IL-2 Antibody

Catalog #: AF-402-NA
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF-402-NA
AF-402-SP

Cell Proliferation Induced by IL‑2 and Neutralization by Mouse IL‑2 Antibody.

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All IL-2 Antibodies

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Citations (2)

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Mouse IL-2 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IL-2 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant rat IL‑2 and recombinant cotton rat IL‑2 is observed and less than 2% cross-reactivity with recombinant human IL‑2, recombinant canine IL‑2, recombinant feline IL‑2, recombinant equine IL‑2, recombinant bovine IL‑2, and recombinant porcine IL‑2 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse IL-2 (R&D Systems, Catalog # 402-ML)
Ala21-Gln169
Accession # P04351

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse IL‑2 (Catalog # 402-ML)

ELISA

This antibody functions as an ELISA detection antibody when paired with Rabbit Anti-Mouse IL‑2 Monoclonal Antibody(Catalog # MAB4022).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Mouse IL-2 DuoSet ELISA Kit (Catalog # DY402) for convenient development of a sandwich ELISA or the Mouse IL-2 Quantikine ELISA Kit (Catalog # M2000) for a complete optimized ELISA.

Neutralization

Measured by its ability to neutralize IL‑2-induced proliferation in the CTLL‑2 mouse cytotoxic T cell line [Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 276]. The Neutralization Dose (ND₅₀) is typically 0.1-0.3 µg/mL in the presence of 2 ng/mL Recombinant Mouse IL‑2.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Cell Proliferation Induced by IL‑2 and Neutralization by Mouse IL‑2 Antibody. Recombinant Mouse IL‑2 (Catalog # 402-ML) stimulates proliferation in the CTLL‑2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-402-NA). The ND₅₀ is typically 0.1-0.3 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-2

Interleukin-2 (IL-2) is an O-glycosylated four alpha -helix bundle cytokine that has potent stimulatory activity for antigen-activated T cells. It is expressed by CD4⁺ and CD8⁺ T cells, gamma δ T cells, B cells, dendritic cells, and eosinophils. Mature mouse IL-2 shares 56% and 73% aa sequence identity with human and rat IL-2, respectively. It shows strain-specific heterogeneity in an N-terminal region that contains a poly-glutamine stretch. Mouse and human IL-2 exhibit cross-species activity. The receptor for IL-2 consists of three subunits that are present on the cell surface in varying preformed complexes. The 55 kDa IL-2 R alpha is specific for IL-2 and binds with low affinity. The 75 kDa IL-2 R beta, which is also a component of the IL-15 receptor, binds IL-2 with intermediate affinity. The 64 kDa common gamma chain gamma c/IL-2 R gamma, which is shared with the receptors for IL-4, -7, -9, -15, and -21, does not independently interact with IL-2. Upon ligand binding, signal transduction is performed by both IL-2 R beta and gamma c. IL-2 is best known for its autocrine and paracrine activity on T cells. It drives resting T cells to proliferate and induces IL-2 and IL-2 R alpha synthesis. It contributes to T cell homeostasis by promoting the Fas-induced death of naïve CD4⁺ T cells but not activated CD4⁺ memory lymphocytes. IL-2 plays a central role in the expansion and maintenance of regulatory T cells, although it inhibits the development of Th17 polarized cells. Thus, IL-2 may be a key cytokine in the natural suppression of autoimmunity.

Long Name

Interleukin 2

Entrez Gene IDs

3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)

Alternate Names

Aldesleukin; IL2; IL-2; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; Proleukin; T cell growth factor; T-cell growth factor; TCGF

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Citations for Mouse IL-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Enhanced production of IL-2 from anti-CD3 antibody-stimulated mouse spleen cells by artepillin C, a major component of Brazilian green propolis
Authors: H Tsuruta, M Mizuno-Kam, M Takahashi, M Ando, K Ikeno, K Ueno, E Takayama, H Kawaki, G Nakamura, T Nikaido, H Fujita, N Kondoh
Journal of oral biosciences, 2022-06-03;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Atherogenic effect of interleukin-2 and antiatherogenic effect of interleukin-2 antibody in apo-E-deficient mice.
Authors: Upadhya S, Mooteri S, Peckham N, Pai RG
Angiology, 2004-05-01;55(3):289-94.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization