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Mouse IL-2 Quantikine ELISA Kit

Catalog #: M2000
54 Citations 7 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
PM2000
SM2000
M2000-1
Mouse IL-2 ELISA Standard Curve
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Product Details
Procedure
Citations (54)
FAQs
Supplemental Products
Reviews (7)
Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Mouse IL-2 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL)
Sensitivity
3 pg/mL
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum)
Specificity
Natural and recombinant mouse IL-2
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse IL-2 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IL-2 levels in cell culture supernates and serum. It contains E. coli-expressed recombinant mouse IL-2 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse IL-2 accurately. Results obtained using natural mouse IL-2 showed dose curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-2.

Precision

Intra-Assay Precision (Precision within an assay)
Inter-Assay Precision (Precision between assays)

Cell Culture Supernates, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 70.4 187 595 70.9 175 559
Standard Deviation 3.9 10 23.4 3.6 9.6 24.5
CV% 5.5 5.3 3.9 5.1 5.5 4.4

Recovery

The recovery of mouse IL-2 spiked to three levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=6) 102 84-110
Serum (n=6) 102 96-108

Linearity

To assess the linearity of the assay, five or more samples containing and/or spiked with various concentrations of mouse IL-2 in each matrix were diluted with Calibrator Diluent and then assayed.
Mouse IL-2 ELISA Linearity

Scientific Data

N/A Mouse IL-2 ELISA Standard Curve View Larger

Mouse IL-2 ELISA Standard Curve

Product Datasheets

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Product Datasheet
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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-2

IL-2 (Interleukin 2) is a critical cytokine in T cell biology. It promotes T cell activation and expansion, the development, maintenance and function of regulatory T cells (Treg), and the differentiation of CD8+ T cells into terminal effector cells and memory cells. IL-2 signals through a receptor complex composed of CD25/IL-2 R alpha, IL-2 R beta, and the Common gamma Chain (gamma c). IL-2 R beta is also a component of the IL-15 receptor complex. Gamma c is also a signaling subunit in the receptors for IL-4, -7, -9, -15, and -21.

Long Name:
Interleukin 2
Entrez Gene IDs:
3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)
Alternate Names:
Aldesleukin; IL2; IL-2; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; Proleukin; T cell growth factor; T-cell growth factor; TCGF
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 50 µL Assay Diluent
  3.   Add 50 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
    6. 100 µL Conjugate
  6.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate and wash 5 times.
    8. 100 µL Substrate Solution
  8.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    9. 100 µL Stop Solution
  9.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Mouse IL-2 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

54 Citations: Showing 1 - 10
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  1. IRF8-mutant B cell lymphoma evades immunity through a CD74-dependent deregulation of antigen processing and presentation in MHC CII complexes
    Authors: Qiu, Z;Khalife, J;Lin, AP;Ethiraj, P;Jaafar, C;Chiou, L;Huelgas-Morales, G;Aslam, S;Arya, S;Gupta, YK;Dahia, PLM;Aguiar, RCT;
    bioRxiv : the preprint server for biology
    Species: Mouse
    Sample Types: Cell Culture Supernates
  2. Immune-Stimulating Potential of Lacticaseibacillus rhamnosus LM1019 in RAW 264.7 Cells and Immunosuppressed Mice Induced by Cyclophosphamide
    Authors: You, Y;Kim, SH;Kim, CH;Kim, IH;Shin, Y;Kim, TR;Sohn, M;Park, J;
    Microorganisms
    Species: Mouse
    Sample Types: Serum
  3. STING signaling activation modulates macrophage polarization via CCL2 in radiation-induced lung injury
    Authors: Ni, J;Guo, T;Zhou, Y;Jiang, S;Zhang, L;Zhu, Z;
    Journal of translational medicine
    Species: Mouse
    Sample Types: BALF, Serum
  4. CLEC-1 is a death sensor that limits antigen cross-presentation by dendritic cells and represents a target for cancer immunotherapy
    Authors: M Drouin, J Saenz, V Gauttier, B Evrard, G Teppaz, S Pengam, C Mary, A Desselle, V Thepenier, E Wilhelm, E Merieau, C Ligeron, I Girault, MD Lopez, C Fourgeux, D Sinha, I Baccelli, A Moreau, C Louvet, R Josien, J Poschmann, N Poirier, E Chiffoleau
    Science Advances, 2022-11-18;8(46):eabo7621.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  5. Immunomodulatory effects of cyclotides isolated from Viola odorata in an experimental autoimmune encephalomyelitis animal model of multiple sclerosis
    Authors: L Dayani, MS Dinani, M Aliomrani, H Hashempour, J Varshosaz, A Taheri
    Oncogene, 2022-06-10;64(0):103958.
    Species: Mouse
    Sample Types: Serum
  6. Commensal oral microbiota induces osteoimmunomodulatory effects separate from systemic microbiome in mice
    Authors: JD Hathaway-S, JD Aartun, NA Poulides, MB Kuhn, BE McCormick, ME Chew, E Huang, RP Darveau, C Westwater, CM Novince
    JCI Insight, 2022-02-22;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  7. Augmenting ATG14 alleviates atherosclerosis and inhibits inflammation via promotion of autophagosome-lysosome fusion in macrophages
    Authors: H Zhang, S Ge, B Ni, K He, P Zhu, X Wu, Y Shao
    Autophagy, 2021-05-04;0(0):.
    Species: Mouse
    Sample Types: Serum
  8. Structures of mouse and human GITR-GITRL complexes reveal unique TNF superfamily interactions
    Authors: F Wang, B Chau, SM West, CR Kimberlin, F Cao, F Schwarz, B Aguilar, M Han, W Morishige, C Bee, G Dollinger, A Rajpal, P Strop
    Nature Communications, 2021-03-02;12(1):1378.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  9. Oral administration of eugenol oleate cures experimental visceral leishmaniasis through cytokines abundance
    Authors: MR Charan Raj, A Kar, S Srinivasan, D Chellappan, J Debnath, S Kar Mahapa
    Cytokine, 2020-10-28;0(0):155301.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  10. Curcumin Enhances the Antitumoral Effect Induced by the Recombinant Vaccinia Neu Vaccine (rV-neuT) in Mice with Transplanted Salivary Gland Carcinoma Cells
    Authors: C Focaccetti, M Benvenuto, S Ciuffa, S Fazi, M Scimeca, A Nardi, MT Miele, A Battisti, E Bonanno, A Modesti, L Masuelli, R Bei
    Nutrients, 2020-05-14;12(5):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  11. CCL11 increases the proportion of CD4+CD25+Foxp3+ Treg cells and the production of IL?2 and TGF?&beta by CD4+ T�cells via the STAT5 signaling pathway
    Authors: R Wang, K Huang
    Mol Med Rep, 2020-04-01;0(0):.
    Species: Mouse
    Sample Types: Serum
  12. Oncolytic activity of HF10 in head and neck squamous cell carcinomas
    Authors: S Esaki, F Goshima, H Ozaki, G Takano, Y Hatano, D Kawakita, K Ijichi, T Watanabe, Y Sato, T Murata, H Iwata, Y Shibamoto, S Murakami, Y Nishiyama, H Kimura
    Cancer Gene Ther., 2019-09-03;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  13. Liver-target nanotechnology facilitates berberine to ameliorate cardio-metabolic diseases
    Authors: HH Guo, CL Feng, WX Zhang, ZG Luo, HJ Zhang, TT Zhang, C Ma, Y Zhan, R Li, S Wu, Z Abliz, C Li, XL Li, XL Ma, LL Wang, WS Zheng, YX Han, JD Jiang
    Nat Commun, 2019-04-30;10(1):1981.
    Species: Mouse
    Sample Types: Plasma
  14. Topical essential fatty acid oil on wounds: Local and systemic effects
    Authors: BG Lania, J Morari, AR Almeida, MND Silva, G Vieira-Dam, KA Lins, CL César, LA Velloso, NB Maia, ML Cintra, PENF Velho
    PLoS ONE, 2019-01-04;14(1):e0210059.
    Species: Mouse
    Sample Types: Serum
  15. Carnosic acid protects mice from high-fat diet-induced NAFLD by regulating MARCKS
    Authors: HM Song, X Li, YY Liu, WP Lu, ZH Cui, L Zhou, D Yao, HM Zhang
    Int. J. Mol. Med., 2018-03-27;0(0):.
    Species: Mouse
    Sample Types: Serum
  16. Glucans and Cancer: Comparison of Commercially Available ?-glucans - Part IV
    Authors: V Vetvicka, J Vetvickova
    Anticancer Res., 2018-03-01;38(3):1327-1333.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  17. Altered gut microbiome promotes proteinuria in mice induced by Adriamycin
    Authors: Q Jiang, X He, Y Zou, Y Ding, H Li, H Chen
    AMB Express, 2018-02-28;8(1):31.
    Species: Mouse
    Sample Types: Serum
  18. Role of the EHD Family of Endocytic Recycling Regulators for TCR Recycling and T Cell Function
    Authors: FM Iseka, BT Goetz, I Mushtaq, W An, LR Cypher, TA Bielecki, EC Tom, P Arya, S Bhattachar, MD Storck, CL Semerad, JE Talmadge, RL Mosley, V Band, H Band
    J. Immunol., 2017-12-06;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  19. Viral RNA-Unprimed Rig-I Restrains Stat3 Activation in the Modulation of Regulatory T Cell/Th17 Cell Balance
    Authors: H Yang, HZ Guo, XY Li, J Lin, W Zhang, JM Zhao, HX Zhang, SJ Chen, Z Chen, J Zhu
    J. Immunol., 2017-05-26;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  20. Interference of the T Cell and Antigen-Presenting Cell Costimulatory Pathway Using CTLA4-Ig (Abatacept) Prevents Staphylococcal Enterotoxin B Pathology
    Authors: SJ Whitfield, C Taylor, JE Risdall, GD Griffiths, JT Jones, ED Williamson, S Rijpkema, L Saraiva, S Vessillier, AC Green, AJ Carter
    J. Immunol, 2017-03-20;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  21. Death receptor 6 contributes to autoimmunity in lupus-prone mice
    Authors: D Fujikura, M Ikesue, T Endo, S Chiba, H Higashi, T Uede
    Nat Commun, 2017-01-03;8(0):13957.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  22. Anti-tumor effects of (1?3)-?-d-glucan from Saccharomyces cerevisiae in S180 tumor-bearing mice
    Authors: Yong Zhan
    Int. J. Biol. Macromol., 2016-11-09;0(0):.
    Species: Mouse
    Sample Types: Serum
  23. Intrafibrillar silicified collagen scaffold modulates monocyte to promote cell homing, angiogenesis and bone regeneration
    Authors: Ji-Hua Chen
    Biomaterials, 2016-10-31;113(0):203-216.
    Species: Mouse
    Sample Types: Plasma
  24. Use of Recombinant Virus Replicon Particles for Vaccination against Mycobacterium ulcerans Disease.
    Authors: Bolz M, Kerber S, Zimmer G, Pluschke G
    PLoS Negl Trop Dis, 2015-08-14;9(8):e0004011.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  25. MDSC and TGFbeta Are Required for Facilitation of Tumor Growth in the Lungs of Mice Exposed to Carbon Nanotubes.
    Authors: Shvedova A, Kisin E, Yanamala N, Tkach A, Gutkin D, Star A, Shurin G, Kagan V, Shurin M
    Cancer Res, 2015-03-05;75(8):1615-23.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  26. IL-1 signaling modulates activation of STAT transcription factors to antagonize retinoic acid signaling and control the TH17 cell-iTreg cell balance.
    Authors: Basu R, Whitley S, Bhaumik S, Zindl C, Schoeb T, Benveniste E, Pear W, Hatton R, Weaver C
    Nat Immunol, 2015-02-02;16(3):286-95.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  27. Celecoxib sensitizes Staphylococcus aureus to antibiotics in macrophages by modulating SIRT1.
    Authors: Annamanedi M, Kalle A
    PLoS ONE, 2014-06-20;9(6):e99285.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  28. Diisopropylamine dichloroacetate, a novel pyruvate dehydrogenase kinase 4 inhibitor, as a potential therapeutic agent for metabolic disorders and multiorgan failure in severe influenza.
    Authors: Yamane, Kazuhiko, Indalao, Irene L, Chida, Junji, Yamamoto, Yoshikaz, Hanawa, Masaaki, Kido, Hiroshi
    PLoS ONE, 2014-05-27;9(5):e98032.
    Species: Mouse
    Sample Types: Tissue Homogenates
  29. Suppression of the macrophage proteasome by ethanol impairs MHC class I antigen processing and presentation.
    Authors: D'Souza, Alain J, Desai, Shyamal, Rudner, Xiaowen, Kelly, Michelle, Ruan, SanBao, Shellito, Judd E
    PLoS ONE, 2013-02-25;8(2):e56890.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  30. The Role of IL-15 in Activating STAT5 and Fine-Tuning IL-17A Production in CD4 T Lymphocytes.
    Authors: Pandiyan P, Yang X, Saravanamuthu S, Zheng L, Ishihara S
    J. Immunol., 2012-09-19;189(9):4237-46.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  31. Activated protein C inhibits pancreatic islet inflammation, stimulates T regulatory cells, and prevents diabetes in non-obese diabetic (NOD) mice.
    Authors: Xue M, Dervish S, Harrison LC, Fulcher G, Jackson CJ
    J. Biol. Chem., 2012-03-23;287(20):16356-64.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  32. Origination of new immunological functions in the costimulatory molecule B7-H3: the role of exon duplication in evolution of the immune system.
    Authors: Sun J, Fu F, Gu W, Yan R, Zhang G, Shen Z, Zhou Y, Wang H, Shen B, Zhang X
    PLoS ONE, 2011-09-13;6(9):e24751.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  33. Control of mouse graft-versus-host disease following allogeneic bone marrow transplantation by blocking the CD28/B7 signaling pathway with lentiviral vector-mediated RNA interference.
    Authors: Sang W, Zhou C, Cheng N, Li Z, Zeng L, Xu K
    Immunol. Lett., 2011-01-26;136(2):194-202.
    Species: Mouse
    Sample Types: Plasma
  34. The adjuvancy of OX40 ligand (CD252) on an HIV-1 canarypox vaccine.
    Authors: Liu J, Ngai N, Stone GW, Yue FY, Ostrowski MA
    Vaccine, 2009-06-30;27(37):5077-84.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  35. Dietary curcumin and limonin suppress CD4+ T-cell proliferation and interleukin-2 production in mice.
    Authors: Kim W, Fan YY, Smith R, Patil B, Jayaprakasha GK, McMurray DN, Chapkin RS
    J. Nutr., 2009-03-25;139(5):1042-8.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  36. Antigen-loaded ER microsomes from APC induce potent immune responses against viral infection.
    Authors: Sofra V, Mansour S, Liu M, Gao B, Primpidou E, Wang P, Li S
    Eur. J. Immunol., 2009-01-01;39(1):85-95.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  37. The LTK63 adjuvant improves protection conferred by Ag85B DNA-protein prime-boosting vaccination against Mycobacterium tuberculosis infection by dampening IFN-gamma response.
    Authors: Palma C, Iona E, Giannoni F, Pardini M, Brunori L, Fattorini L, Del Giudice G, Cassone A
    Vaccine, 2008-06-12;26(33):4237-43.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  38. Thymic stromal lymphopoietin and thymic stromal lymphopoietin-conditioned dendritic cells induce regulatory T-cell differentiation and protection of NOD mice against diabetes.
    Authors: Besin G, Gaudreau S, Menard M, Guindi C, Dupuis G, Amrani A
    Diabetes, 2008-05-13;57(8):2107-17.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  39. Dendritic cells derived from TBP-2-deficient mice are defective in inducing T cell responses.
    Authors: Son A, Nakamura H, Okuyama H, Oka S, Yoshihara E, Liu W, Matsuo Y, Kondo N, Masutani H, Ishii Y, Iyoda T, Inaba K, Yodoi J
    Eur. J. Immunol., 2008-05-01;38(5):1358-67.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  40. Lipopolysaccharide-activated IL-10-secreting dendritic cells suppress experimental autoimmune uveoretinitis by MHCII-dependent activation of CD62L-expressing regulatory T cells.
    Authors: Lau AW, Biester S, Cornall RJ, Forrester JV
    J. Immunol., 2008-03-15;180(6):3889-99.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  41. The endothelial cell-produced antiangiogenic cytokine vascular endothelial growth inhibitor induces dendritic cell maturation.
    Authors: Tian F, Grimaldo S, Fujita M, Fugita M, Cutts J, Vujanovic NL, Li LY
    J. Immunol., 2007-09-15;179(6):3742-51.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  42. Granulocyte-macrophage colony-stimulating factor prevents diabetes development in NOD mice by inducing tolerogenic dendritic cells that sustain the suppressive function of CD4+CD25+ regulatory T cells.
    Authors: Gaudreau S, Guindi C, Menard M, Besin G, Dupuis G, Amrani A
    J. Immunol., 2007-09-15;179(6):3638-47.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  43. Suppression of established experimental autoimmune uveitis by anti-LFA-1alpha Ab.
    Authors: Ke Y, Sun D, Zhang P, Jiang G, Kaplan HJ, Shao H
    Invest. Ophthalmol. Vis. Sci., 2007-06-01;48(6):2667-75.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  44. Interleukin-2 gene variation impairs regulatory T cell function and causes autoimmunity.
    Authors: Yamanouchi J, Rainbow D, Serra P, Howlett S, Hunter K, Garner VE, Gonzalez-Munoz A, Clark J, Veijola R, Cubbon R, Chen SL, Rosa R, Cumiskey AM, Serreze DV, Gregory S, Rogers J, Lyons PA, Healy B, Smink LJ, Todd JA, Peterson LB, Wicker LS, Santamaria P
    Nat. Genet., 2007-02-04;39(3):329-37.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  45. Intercellular adhesion molecule-1 expression is required on multiple cell types for the development of experimental autoimmune encephalomyelitis.
    Authors: Bullard DC, Hu X, Schoeb TR, Collins RG, Beaudet AL, Barnum SR
    J. Immunol., 2007-01-15;178(2):851-7.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  46. Tetrathiomolybdate is effective in a mouse model of arthritis.
    Authors: McCubbin MD, Hou G, Abrams GD, Dick R, Brewer GJ
    J. Rheumatol., 2006-10-15;33(12):2501-6.
    Species: Mouse
    Sample Types: Serum
  47. Blockade of transforming growth factor-{beta} signaling in tumor-reactive CD8(+) T cells activates the antitumor immune response cycle.
    Authors: Zhang Q, Yang XJ, Kundu SD, Pins M, Javonovic B, Meyer R, Kim SJ, Greenberg NM, Kuzel T, Meagher R, Guo Y, Lee C
    Mol. Cancer Ther., 2006-07-01;5(7):1733-43.
    Species: Mouse
    Sample Types: Serum
  48. Immunosuppressant triptolide inhibits dendritic cell-mediated chemoattraction of neutrophils and T cells through inhibiting Stat3 phosphorylation and NF-kappaB activation.
    Authors: Liu Q, Chen T, Chen G, Li N, Wang J, Ma P, Cao X
    Biochem. Biophys. Res. Commun., 2006-05-12;345(3):1122-30.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  49. PKCtheta and PKA are antagonistic partners in the NF-AT transactivation pathway of primary mouse CD3+ T lymphocytes.
    Authors: Hermann-Kleiter N, Thuille N, Pfeifhofer C, Gruber T, Schafer M, Zitt C, Hatzelmann A, Schudt C, Leitges M, Baier G
    Blood, 2006-03-02;107(12):4841-8.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  50. Increased interleukin-10 associated with low IL-6 concentration correlated with greater survival rates in mice infected by rabies virus vaccinated against it and immunomodulated with P. acnes.
    Authors: Megid J, Kaneno R, Nozaki CN, Brito CJ, Almeida MF
    Comp. Immunol. Microbiol. Infect. Dis., 2004-11-01;27(6):393-411.
    Species: Mouse
    Sample Types: Serum
  51. EphrinB1 is essential in T-cell-T-cell co-operation during T-cell activation.
    Authors: Yu G, Luo H, Wu Y, Wu J
    J. Biol. Chem., 2004-10-22;279(53):55531-9.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  52. Dopamine, through the extracellular signal-regulated kinase pathway, downregulates CD4+CD25+ regulatory T-cell activity: implications for neurodegeneration.
    Authors: Kipnis J, Cardon M, Avidan H, Lewitus GM, Mordechay S, Rolls A, Shani Y, Schwartz M
    J. Neurosci., 2004-07-07;24(27):6133-43.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  53. Anti-tumor mechanism of Z-100, an immunomodulatory Arabinomannan extracted from Mycobacterium tuberculosis strain Aoyama B, on pulmonary metastases of B16F10 melanoma: restoration of helper T cell responses via suppression of glucocorticoid-genesis.
    Authors: Oka H, Emori Y, Sasaki H, Shiraishi Y, Yoshinaga K, Kurimoto T
    Microbiol. Immunol., 2002-01-01;46(5):343-51.
  54. The protein tyrosine kinase inhibitor AG126 prevents the massive microglial cytokine induction by pneumococcal cell walls.
    Authors: Hanisch UK, Prinz M, Angstwurm K, Hausler KG, Kann O, Kettenmann H, Weber JR
    Eur. J. Immunol., 2001-07-01;31(7):2104-15.
    Species: Mouse
    Sample Types: Cell Culture Supernates

FAQs

  1. Assay Diluent RD1-14 contains visible precipitate. Is this acceptable to use?

    • A small amount of precipitate is expected and acceptable in Assay Diluent RD1-14. Variability has been observed between vials and diluent lots. It may not fully resolubilize but this will not impact assay performance. It is not recommended to filter the precipitate.

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Average Rating: 4.4 (Based on 7 Reviews)

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Mouse IL-2 Quantikine ELISA Kit
By Anonymous on 12/12/2022
Sample Tested: Cell culture supernatant

Virus-infected Cell culture supernatant diluted 1:10,000

Mouse IL-2 Quantikine ELISA Kit M2000
Mouse IL-2 Quantikine ELISA Kit
By Thanh Nguyen on 10/18/2020
Sample Tested: activated mouse CD8 T cell
Mouse IL-2 Quantikine ELISA Kit
By Dhivya Sridaran on 04/04/2019
Sample Tested: Jurkat human acute T cell leukemia cell line,MOLT-4 human acute lymphoblastic leukemia cell line
Mouse IL-2 Quantikine ELISA Kit
By Shambhavi Bhagwat on 03/21/2019
Sample Tested: activated mouse CD8 T cell
Mouse IL-2 Quantikine ELISA Kit
By Anonymous on 06/19/2018
Sample Tested: Serum
Mouse IL-2 Quantikine ELISA Kit
By Anonymous on 06/12/2018
Sample Tested: Serum
Mouse IL-2 Quantikine ELISA Kit
By Hannah White on 05/24/2017
Sample Tested: Concanavalin A-stimulated splenocytes