Mouse IL-27 R alpha /WSX-1/TCCR Antibody

Catalog #: MAB2109 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2109
MAB2109-SP

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IL-27 R alpha /WSX-1/TCCR Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IL-27 R alpha /WSX‑1/TCCR in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse gp130 or recombinant human IL-27 R alpha is observed.

Source

Monoclonal Rat IgG_2A Clone # 263517

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse IL-27 R alpha /WSX‑1/TCCR
Gly29-Lys510
Accession # O70394

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse IL-27 R alpha /WSX‑1/TCCR Fc Chimera (Catalog # 2109-TC)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-27 R alpha/WSX-1/TCCR

IL‑27 R alpha (also known as WSX‑1 and TCCR) is a 85‑95 kDa member of the type I, group 2 cytokine receptor family (1‑6). Mature IL‑27 R alpha is a type I transmembrane glycoprotein that contains a 486 amino acid (aa) extracellular region, a 21 aa transmembrane segment and a 92 aa cytoplasmic domain. Consistent with type I cytokine receptors, the extracellular region contains four positionally conserved cysteine residues, a WSxWS motif (for receptor folding and ligand binding), and three fibronectin type III repeats. The intracellular domain contains a “box‑1” motif that may be involved with Janus kinases (3). In mouse, a soluble 33 kDa splice form that shows a 20 aa substitution for aa 251‑623 has been identified (7). The mouse IL‑27 R alpha extracellular region shares 63% amino acid identity with the human IL‑27 R alpha extracellular domain (2, 3). IL‑27 R alpha is expressed in mast cells, endothelial cells, NK cells, macrophages, monocytes, B cells, dendritic cells, and naïve T cells (1, 2, 4, 8). Typical of other class I cytokine receptor chains, the ligand binding IL‑27 R alpha molecule is known to heterodimerize with a signal‑transducing subunit (gp130) to form a functional IL‑27 receptor (9, 10). In addition, IL‑27 R alpha is reported to complex with CNTFR alpha and gp130 form a humanin receptor on neurons (7, 11), and to complex with gp130 and IL‑6 R to form a receptor for a p28:CLF heterodimeric cytokine on lymphocytes (12). Studies using IL‑27 R alpha /WSX‑1^-/- mice reveal that IL‑27 has the ability to suppress T cell activity during infection, and to mediate an inhibition of both type 1 and type 2 T cell immunity (4, 13, 14). In particular, IL‑27 is known to act on naïve T cells, blocking their differentiation into a Th17 phenotype. Notably, cells committed to a Th17 phenotype, although they express a functional IL‑27 receptor, are unresponsive to the effects of IL‑27 (15). Activated T cells that are CD4+ and CD8+, and which express the IL‑27 receptor, can be induced by IL‑27 to form a double‑positive CD25⁺ FoxP3^- IFN‑ gamma plus IL‑10 secreting phenotype that both promotes and suppresses the inflammatory response (16).

References

Villarino, A.V. et al. (2004) J. Immunol. 173:715.
Chen, Q. et al. (2000) Nature 407:916.
Sprecher, C.A. et al. (1998) Biochem. Biophys. Res. Commun. 246:82.
Artis, D. et al. (2004) J. Immunol. 173:5626.
Yoshida, H. and Y. Miyazaki (2008) Int. J. Biochem. Cell Biol. 40:2379.
Yoshida, H. and M. Yoshiyuki (2008) Immunol. Rev. 226:234.
Hashimoto, Y. et al. (2009) Biochem. Biophys. Res. Commun. 389:95
Holscher, C. et al. (2005) J. Immunol. 174:3534.
Pflanz, S. et al. (2004) J. Immunol. 172:2225.
Scheller, J. et al. (2005) Biochem. Biophys. Res. Commun. 326:724.
Hashimoto, Y. et al. (2009) Mol. Biol. Cell 20:2864.
Crabe, S. et al. (2009) J. Immunol. 183:7692.
Villarino, A. et al. (2003) J. Immunol. 170:645.
Hamano., S. et al. (2003) Immunity 19:657.
El-behi, M. et al. (2009) J. Immunol. 183:4957.
Fitzgerald, D.C. et al. (2007) Nat. Immunol. 8:1372.

Long Name

Interleukin-27 Receptor Subunit alpha

Entrez Gene IDs

9466 (Human); 50931 (Mouse)

Alternate Names

class I cytokine receptor; CRL1IL-27R; Cytokine receptor-like 1; IL-27 R alpha; IL27R alpha; IL27R; IL27RA; IL-27Ra; IL-27R-alpha; interleukin 27 receptor, alpha; interleukin-27 receptor subunit alpha; TCCR; TCCRIL-27 receptor subunit alpha; T-cell cytokine receptor type 1; Type I T-cell cytokine receptor; WSX-1; WSX1IL-27R subunit alpha; zcytor1