Mouse IL-3R alpha /CD123 Antibody

Catalog #: AF983 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF983
AF983-SP

All IL-3R alpha Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IL-3R alpha /CD123 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IL‑3 R alpha /CD123 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant human IL-3 R alpha and recombinant mouse IL-3 R beta is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse IL‑3 R alpha /CD123
Ser17-Lys331
Accession # P26952

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Purity

Immunogen affinity purified

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse IL‑3 R alpha /CD123 Fc Chimera (Catalog # 983-MR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-3R alpha

Interleukin 3 (IL-3) is a pleiotropic cytokine produced primarily by activated T cells or mast cells. IL-3 stimulates the proliferation and differentiation of hemopoietic cells including the pluripotent hematopoietic stem cells as well as various lineage-committed cells. The biological effects of IL-3 on the various cell types are mediated by the binding of IL-3 to specific cell surface receptor complexes. The functional high-affinity IL-3 receptor is a heterodimer consisting of a ligand binding alpha subunit and the beta subunit. The alpha subunit alone binds IL-3 with low affinity. The beta subunit is required for the high-affinity binding of IL-3 to the heterodimeric receptor complex. The beta subunit has also been found to be a component of the high-affinity receptor complex for IL-5 and GM-CSF and is also referred to as the beta common ( beta c) chain. In the mouse, there are two IL-3 R beta proteins. The first identified mouse IL-3 R beta was also called AIC2A and binds IL-3 with low affinity (1). The second mIL-3 R beta was referred to as AIC2B (2). AIC2B doesn’t bind IL-3 and is the homolog of the human IL-3 R beta. AIC2A was found to be the result of a gene duplication event. The mouse IL-3 R alpha, also called SUT-1, will form complexes with either mouse IL-3 R beta protein (3). Both the alpha and the beta subunits are members of the cytokine receptor superfamily.

References

Itoh, N. et al. (1990) Science 247:324.
Gorman, D.M. et al. (1990) Proc. Natl. Acad. Sci. USA 87:5459.
Hara, T. and A. Miyajima (1992) EMBO J. 11:1875.
Schrader, J.W. (2001) Cytokine Reference, Oppenheim, J.J. and M. Feldmann, eds, Academic Press, New York, p. 1899.

Long Name

Interleukin 3 Receptor alpha

Entrez Gene IDs

3563 (Human); 16188 (Mouse); 102138639 (Cynomolgus Monkey)

Alternate Names

CD123 antigen; CD123; hIL3Ra; hIL-3Ra; IL-3 R alpha; IL-3 receptor subunit alpha; IL3R alpha; IL-3R alpha; IL-3R subunit alpha; IL3R; IL3RA; IL-3Ra; IL-3R-alpha; IL3RAY; IL3RX; IL3RY; interleukin 3 receptor, alpha (low affinity); interleukin-3 receptor subunit alpha; MGC34174