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Mouse IL-4 MAb (Clone 30340)

Catalog #: MAB404
26 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB404-SP
MAB404-100
MAB404-500
Cell Proliferation Induced by IL‑4 and Neutralization by Mouse IL‑4 Antibody.
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Product Details
Citations (26)
FAQs
Supplemental Products
Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IL-4 MAb (Clone 30340) Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-4 in ELISAs and Western blots.
Source
Monoclonal Rat IgG1 Clone # 30340
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse IL-4
His23-Ser140
Accession # P07750
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse IL‑4 (Catalog # 404-ML)
under non-reducing conditions only

Mouse IL-4 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Mouse IL‑4 Biotinylated Antibody (Catalog # BAF404)

Standard: Recombinant Mouse IL-4 Protein (Catalog # 404-ML)

Neutralization
Measured by its ability to neutralize IL‑4-induced proliferation in the HT‑2 mouse T cell line. The Neutralization Dose (ND50) is typically 0.1-0.6 µg/mL in the presence of 7.5 ng/mL Recombinant Mouse IL‑4.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by IL‑4 and Neutralization by Mouse IL‑4 Antibody. View Larger

Cell Proliferation Induced by IL‑4 and Neutralization by Mouse IL‑4 Antibody. Recombinant Mouse IL-4 (Catalog # 404-ML) stimulates proliferation in the HT-2 mouse T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat Anti-Mouse IL-4 (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL-4 Monoclonal Antibody (Catalog # MAB404). The ND50 is typically 0.1-0.6 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-4

Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13-18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1‑4). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (5). Mouse IL-4 is synthesized with a 24 amino acid (aa) signal sequence. Mature mouse IL-4 shares 39%, 39%, and 59% aa sequence identity with bovine, human, and rat IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities (6-8). IL-4 exerts its effects through two receptor complexes (9, 10). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgG1 and IgE in mouse B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (11‑14). IL-4 plays a dominant role in the development of allergic inflammation and asthma (13, 15).

References
  1. Benczik, M. and S.L. Gaffen (2004) Immunol. Invest. 33:109.
  2. Chomarat, P. and J. Banchereau (1998) Int. Rev. Immunol. 17:1.
  3. Lee, F. et al. (1986) Proc. Natl. Acad. Sci. USA 83:2061.
  4. Noma, Y. et al. (1986) Nature 319:640.
  5. Redfield, C. et al. (1991) Biochemistry 30:11029.
  6. Ramirez, F. et al. (1988) J. Immunol. Meth. 221:141.
  7. Leitenberg, D. and T.L. Feldbush (1988) Cell. Immunol. 111:451.
  8. Mosman, T.R. et al. (1987) J. Immunol. 138:1813.
  9. Mueller, T.D. et al. (2002) Biochim. Biophys. Acta 1592:237.
  10. Nelms, K. et al. (1999) Annu. Rev. Immunol. 17:701.
  11. Paludan, S.R. (1998) Scand. J. Immunol. 48:459.
  12. Corthay, A. (2006) Scand. J. Immunol. 64:93.
  13. Ryan, J.J. et al. (2007) Crit. Rev. Immunol. 27:15.
  14. Grone, A. (2002) Vet. Immunol. Immunopathol. 88:1.
  15. Rosenberg, H.F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.
Long Name
Interleukin 4
Entrez Gene IDs
3565 (Human); 16189 (Mouse); 287287 (Rat); 397225 (Porcine); 280824 (Bovine); 403785 (Canine); 574281 (Primate); 100302454 (Rabbit)
Alternate Names
B cell growth factor 1; BCDF; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; IL4; IL-4; IL-4B_cell stimulatory factor 1; IL4E12; interleukin 4; interleukin-4; Lymphocyte stimulatory factor 1; MGC79402; pitrakinra

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Citations for Mouse IL-4 MAb (Clone 30340)

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

26 Citations: Showing 1 - 10
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  1. Interleukin-4 promotes microglial polarization toward a neuroprotective phenotype after retinal ischemia/reperfusion injury
    Authors: D Chen, C Peng, XM Ding, Y Wu, CJ Zeng, L Xu, WY Guo
    Oncogene, 2022-12-01;17(12):2755-2760.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. EC-18 prevents autoimmune arthritis by suppressing inflammatory cytokines and osteoclastogenesis
    Authors: JS Park, SC Yang, HY Jeong, SY Lee, JG Ryu, JW Choi, HY Kang, SM Kim, SH Hwang, ML Cho, SH Park
    Arthritis Research & Therapy, 2022-11-17;24(1):254.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  3. Depletion of Foxp3+ regulatory T cells augments CD4+ T cell immune responses in atherosclerosis-prone hypercholesterolemic mice
    Authors: K Kasahara, N Sasaki, HZ Amin, T Tanaka, S Horibe, T Yamashita, KI Hirata, Y Rikitake
    Heliyon, 2022-07-19;8(7):e09981.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Capture
  4. The YAP/HIF-1&alpha/miR-182/EGR2 axis is implicated in asthma severity through the control of Th17 cell differentiation
    Authors: J Zhou, N Zhang, W Zhang, C Lu, F Xu
    Cell & bioscience, 2021-05-12;11(1):84.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  5. Ablation of RhoA impairs Th17 cell differentiation and alleviates house dust mite-triggered allergic airway inflammation
    Authors: JQ Yang, KW Kalim, Y Li, Y Zheng, F Guo
    J. Leukoc. Biol., 2019-07-01;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Intraperitoneal infusion of mesenchymal stem cell attenuates severity of collagen antibody induced arthritis
    Authors: Y Nam, SM Jung, YA Rim, H Jung, K Lee, N Park, J Kim, Y Jang, YB Park, SH Park, JH Ju
    PLoS ONE, 2018-06-07;13(6):e0198740.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay
  7. Characterization of the immune response elicited by the vaccinia virus L3 protein delivered as naked DNA
    Authors: M Ramírez, S Santos, O Martínez, R Rodríguez, E Miranda, WD Ramos-Pere, M Otero
    Vaccine, 2018-03-07;36(15):2049-2055.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ELISA Capture
  8. Armc5 deletion causes developmental defects and compromises T-cell immune responses
    Authors: Y Hu, L Lao, J Mao, W Jin, H Luo, T Charpentie, S Qi, J Peng, B Hu, MM Marcinkiew, A Lamarre, J Wu
    Nat Commun, 2017-02-07;8(0):13834.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  9. Targeting the binding interface on a shared receptor subunit of a cytokine family enables the inhibition of multiple member cytokines with selectable target spectrum.
    Authors: Nata T, Basheer A, Cocchi F, van Besien R, Massoud R, Jacobson S, Azimi N, Tagaya Y
    J Biol Chem, 2015-07-16;290(37):22338-51.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  10. Mesenchymal stem/stromal cells protect against autoimmunity via CCL2-dependent recruitment of myeloid-derived suppressor cells.
    Authors: Lee H, Ko J, Jeong H, Ko A, Kim M, Wee W, Yoon S, Oh J
    J Immunol, 2015-03-13;194(8):3634-45.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  11. ROR inverse agonist suppresses insulitis and prevents hyperglycemia in a mouse model of type 1 diabetes.
    Authors: Solt L, Banerjee S, Campbell S, Kamenecka T, Burris T
    Endocrinology, 2015-01-05;156(3):869-81.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  12. TLR2 engagement on CD4(+) T cells enhances effector functions and protective responses to Mycobacterium tuberculosis.
    Authors: Reba S, Li Q, Onwuzulike S, Ding X, Karim A, Hernandez Y, Fulton S, Harding C, Lancioni C, Nagy N, Rodriguez M, Wearsch P, Rojas R
    Eur J Immunol, 2014-03-14;44(5):1410-21.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  13. To investigate the necessity of STRA6 upregulation in T cells during T cell immune responses.
    Authors: Terra R, Wang X, Hu Y, Charpentier T, Lamarre A, Zhong M, Sun H, Mao J, Qi S, Luo H, Wu J
    PLoS ONE, 2013-12-31;8(12):e82808.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  14. Basophils control T-cell responses and limit disease activity in experimental murine colitis.
    Authors: Gomez M, Talke Y, Hofmann C, Ketelsen I, Hermann F, Reich B, Goebel N, Schmidbauer K, Dunger N, Bruhl H, Renner K, Syed S, Mack M
    Mucosal Immunol, 2013-06-12;7(1):188-99.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  15. CCL25 induces alpha 4 beta 7 integrin-dependent migration of IL-17+ gamma/delta T lymphocytes during an allergic reaction.
    Authors: Costa MF, Bornstein VU, Candea AL, Henriques-Pons A, Henriques MG, Penido C
    Eur. J. Immunol., 2012-05-01;42(5):1250-60.
    Species: Mouse
    Sample Types: Pleural Lavage Fluid
    Applications: ELISA Development
  16. Response patterns of cytokines/chemokines in two murine strains after irradiation.
    Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
    Cytokine, 2012-01-25;58(2):169-77.
    Species: Mouse
    Sample Types: Plasma
    Applications: Luminex Development
  17. The effect of conditional EFNB1 deletion in the T cell compartment on T cell development and function.
    Authors: Jin W, Qi S, Luo H
    BMC Immunol., 2011-12-19;12(0):68.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  18. ST2 deficient mice display a normal host defense against pulmonary infection with Mycobacterium tuberculosis.
    Authors: Wieland CW, van der Windt GJ, Florquin S, McKenzie AN, van der Poll T
    Microbes Infect., 2009-03-13;11(4):524-30.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  19. TH17 cells mediate steroid-resistant airway inflammation and airway hyperresponsiveness in mice.
    Authors: McKinley L, Alcorn JF, Peterson A, Dupont RB, Kapadia S, Logar A, Henry A, Irvin CG, Piganelli JD, Ray A, Kolls JK
    J. Immunol., 2008-09-15;181(6):4089-97.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  20. Mitochondrial structural changes and dysfunction are associated with experimental allergic asthma.
    Authors: Mabalirajan U, Dinda AK, Kumar S, Roshan R, Gupta P, Sharma SK, Ghosh B
    J. Immunol., 2008-09-01;181(5):3540-8.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  21. Role of Valpha14+ NKT cells in the development of Hepatitis B virus-specific CTL: activation of Valpha14+ NKT cells promotes the breakage of CTL tolerance.
    Authors: Ito H, Ando K, Ishikawa T
    Int. Immunol., 2008-05-16;20(7):869-79.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  22. CD14 contributes to pulmonary inflammation and mortality during murine tuberculosis.
    Authors: Wieland CW, van der Windt GJ, Wiersinga WJ, Florquin S, van der Poll T
    Immunology, 2008-04-03;125(2):272-9.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development
  23. Cytopiloyne, a novel polyacetylenic glucoside from Bidens pilosa, functions as a T helper cell modulator.
    Authors: Chiang YM, Chang CL, Chang SL, Yang WC, Shyur LF
    J Ethnopharmacol, 2006-10-19;110(3):532-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  24. Severe sepsis exacerbates cell-mediated immunity in the lung due to an altered dendritic cell cytokine profile.
    Authors: Wen H, Hogaboam CM, Gauldie J, Kunkel SL
    Am. J. Pathol., 2006-06-01;168(6):1940-50.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development
  25. Attenuation of autoimmune disease in Fas-deficient mice by treatment with a cytotoxic benzodiazepine.
    Authors: Bednarski JJ, Warner RE, Rao T
    Arthritis Rheum., 2003-03-01;48(3):757-66.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ELISpot Development
  26. AMD3100, a CxCR4 antagonist, attenuates allergic lung inflammation and airway hyperreactivity.
    Authors: Lukacs NW, Berlin A, Schols D, Skerlj RT, Bridger GJ
    Am. J. Pathol., 2002-04-01;160(4):1353-60.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development

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Mouse IL-4 MAb (Clone 30340)
By Anonymous on 11/30/2018
Application: ELISA Sample Tested: Purified Standard Species: Mouse
Mouse IL-4 Antibody
By Michelle Chen on 05/10/2017
Application: Func Sample Tested: Peripheral blood neutrophils Species: Human