Mouse IL-7 Antibody

Catalog #: AF407
7 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF407
AF407-SP

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Cell Proliferation Induced by IL‑7 and Neutralization by Mouse IL‑7 Antibody.

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All IL-7 Antibodies

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Citations (7)

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Mouse IL-7 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IL-7 in ELISAs and Western blots. In sandwich immunoassays, approximately 20% cross-reactivity with recombinant rat IL-7 is observed, and less than 0.1% cross‑reactivity with recombinant human IL-7 is observed. Neutralizes the biological activity of recombinant mouse IL-7. It will not neutralize the biological activity of recombinant human IL-7.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse IL-7
Glu26-Ile154
Accession # Q544C8

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Purity

Immunogen affinity purified

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse IL‑7 (Catalog # 407-ML)

Mouse IL-7 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

0.2-0.8 µg/mL

Use in combination with:

Detection Reagent: Mouse IL‑7 Biotinylated Antibody (Catalog # BAF407)

Standard: Recombinant Mouse IL-7 Protein (Catalog # 407-ML)

Neutralization

Measured by its ability to neutralize IL‑7-induced proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC). The Neutralization Dose (ND₅₀) is typically 0.2-0.4 µg/mL in the presence of 1.5 ng/mL Recombinant Mouse IL‑7.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Cell Proliferation Induced by IL‑7 and Neutralization by Mouse IL‑7 Antibody. Recombinant Mouse IL-7 (Catalog # 407-ML) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-7 (1.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF407). The ND₅₀ is typically 0.2-0.4 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-7

IL-7, previously known as pre-B-cell growth factor and lymphopoietin-1, was originally purified on the basis of its ability to promote the proliferation of precursor B‑cells. It has now been shown that IL-7 can also stimulate the proliferation of thymocytes, T cell progenitors and mature CD4⁺and CD8⁺ T cells. IL-7 can induce the formation of lymphokine-activated killer (LAK) cells as well as the development of cytotoxic T lymphocytes (CTL). IL-7 was also shown to induce the V(D)J rearrangement of the T cell receptor beta gene in mouse fetal thymocytes. Among myeloid lineage cells, IL-7 can up-regulate the production of pro-inflammatory cytokines and stimulate the tumorocidal activity of monocytes/macrophages. IL-7 is expressed by adherent stromal cells from various tissues.

Mouse IL-7 cDNA encodes a precursor protein of 154 amino residues containing a 25 amino acid residue signal peptide. Human IL-7 has approximately 65% amino acid sequence identity with mouse IL-7 and both proteins exhibit cross-species activity.

IL-7 bioactivities are mediated by the binding of IL-7 to functional high-affinity receptor complexes. The ligand binding subunit (IL-7 R) of the IL-7 receptor complex has been cloned from human and mouse sources. In addition to the membrane-anchored form of the IL-7 receptor, a human cDNA clone that encodes a soluble form of the IL-7 R has also been isolated. The gamma chain of the IL-2 receptor complex has been shown to be an essential component for IL-7 signal transduction. Both IL-7 R and IL-2 R gamma are members of the hematopoietin receptor superfamily. Cells known to express IL-7 receptors include pre-B cells, T cells and bone marrow cells.

Long Name

Interleukin 7

Entrez Gene IDs

3574 (Human); 16196 (Mouse); 25647 (Rat)

Alternate Names

IL7; IL-7; IL-7interleukin-7; interleukin 7; Lymphopoietin-1; PBGF

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Product Datasheet

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Citations for Mouse IL-7 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Blockade of IL-7 signaling suppresses inflammatory responses and reverses alopecia areata in C3H/HeJ mice
Authors: Z Dai, EHC Wang, L Petukhova, Y Chang, EY Lee, AM Christiano
Science Advances, 2021-04-02;7(14):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Commensal microflora and interferon-gamma promote steady-state interleukin-7 production in vivo.
Authors: Shalapour S, Deiser K, Sercan O, Tuckermann J, Minnich K, Willimsky G, Blankenstein T, Hammerling GJ, Arnold B, Schuler T
Eur. J. Immunol., 2010-09-01;40(9):2391-400.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC
BCL6 is critical for the development of a diverse primary B cell repertoire.
Authors: Duy C, Yu JJ, Nahar R, Swaminathan S, Kweon SM, Polo JM, Valls E, Klemm L, Shojaee S, Cerchietti L, Schuh W, Jack HM, Hurtz C, Ramezani-Rad P, Herzog S, Jumaa H, Koeffler HP, de Alboran IM, Melnick AM, Ye BH, Muschen M
J. Exp. Med., 2010-05-24;207(6):1209-21.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization
Thymic regulation of autoimmune disease by accelerated differentiation of Foxp3+ regulatory T cells through IL-7 signaling pathway.
Authors: Chen X, Fang L, Song S, Guo TB, Liu A, Zhang JZ
J. Immunol., 2009-10-19;183(10):6135-44.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization
Osteoblasts support B-lymphocyte commitment and differentiation from hematopoietic stem cells.
Authors: Zhu J, Garrett R, Jung Y, Zhang Y, Kim N, Wang J, Joe GJ, Hexner E, Choi Y, Taichman RS, Emerson SG
Blood, 2007-01-16;109(9):3706-12.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization
Murine and human IL-7 activate STAT5 and induce proliferation of normal human pro-B cells.
Authors: Johnson SE, Shah N, Panoskaltsis-Mortari A, Lebien TW
J. Immunol., 2005-12-01;175(11):7325-31.
Species: Mouse
Sample Types: Cell Culture Supernates, Whole Cells
Applications: ELISA Development, Neutralization
A recombinant single-chain IL-7/HGFbeta hybrid cytokine induces juxtacrine interactions of the IL-7 and HGF (c-Met) receptors and stimulates the proliferation of CFU-S12, CLPs, and pre-pro-B cells.
Authors: Lai L, Zeff RA, Goldschneider I
Blood, 2005-11-22;107(5):1776-84.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot