Mouse Integrin alpha 7 Antibody

Catalog #: MAB3518
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3518
MAB3518-SP

Detection of Integrin alpha 7 in C2C12 Mouse Cell Line by Flow Cytometry.

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Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Integrin alpha 7 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Integrin alpha 7 in direct ELISAs. Reactivity with other Integrin alpha 7 isoforms was not tested. In direct ELISAs, no cross-reactivity with recombinant mouse Integrin alpha 2, alpha 3, alpha 4, alpha 5, alpha 9, alpha E, alpha X, recombinant human Integrin alpha L, or alpha 11 is observed.

Source

Monoclonal Rat IgG_2B Clone # 334908

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin alpha 7
Phe34-Pro1036
Accession # NP_032424

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Integrin alpha 7 in C2C12 Mouse Cell Line by Flow Cytometry. C2C12 mouse myoblast cell line was stained with Rat Anti-Mouse Integrin a7 Monoclonal Antibody (Catalog # MAB3518, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 7

Integrin alpha 7 is a transmembrane glycoprotein that forms heterodimers with Integrin beta 1 to bind Laminin 1.

Entrez Gene IDs

3679 (Human); 16404 (Mouse); 81008 (Rat); 102130741 (Cynomolgus Monkey)

Alternate Names

FLJ25220; integrin alpha 7 chain; Integrin alpha 7; integrin alpha-7; integrin, alpha 7; ITGA7

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citations for Mouse Integrin alpha 7 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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PEDF Expression Affects the Oxidative and Inflammatory State of Choroidal Endothelial Cells
Authors: M Farnoodian, CM Sorenson, N Sheibani
Am. J. Physiol., Cell Physiol., 2018-01-10;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Identification of an IFN-gamma/mast cell axis in a mouse model of chronic asthma.
Authors: Yu M, Eckart MR, Morgan AA, Mukai K, Butte AJ, Tsai M, Galli SJ
J. Clin. Invest., 2011-08-01;121(8):3133-43.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Development