Mouse Integrin alpha E/CD103 Antibody

Catalog # Availability Size / Price Qty
AF1990
AF1990-SP
Product Details
Citations (4)
FAQs
Supplemental Products
Reviews (1)

Mouse Integrin alpha E/CD103 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Integrin alpha E/CD103 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Integrin alpha E/CD103 Phe20-Arg1113 Accession # Q60677
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Integrin  alpha E/CD103
Flow Cytometry
2.5 µg/106 cells
Mouse splenocytes
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Background: Integrin alpha E/CD103

The alpha E subunit, also known as CD103, forms a non-covalent heterodimer with Integrin beta 7. Integrin alpha E/ beta 7 is a receptor for E-Cadherin. Integrin alpha E is expressed in intra-epithelial lymphocytes and mediates adhesion of intra-epithelial lymphocytes to epithelial cell monolayers.

Entrez Gene IDs
3682 (Human); 16407 (Mouse); 83577 (Rat)
Alternate Names
antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide; CD103 antigen; CD103; HML-1 antigen; HUMINAE; Integrin alpha E; integrin alpha-E; Integrin alpha-IEL; integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alphapolypeptide); ITGAE; MGC141996; Mucosal lymphocyte 1 antigen

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Citations for Mouse Integrin alpha E/CD103 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. A liver immune rheostat regulates CD8 T cell immunity in chronic HBV infection
    Authors: Bosch, M;Kallin, N;Donakonda, S;Zhang, JD;Wintersteller, H;Hegenbarth, S;Heim, K;Ramirez, C;Fürst, A;Lattouf, EI;Feuerherd, M;Chattopadhyay, S;Kumpesa, N;Griesser, V;Hoflack, JC;Siebourg-Polster, J;Mogler, C;Swadling, L;Pallett, LJ;Meiser, P;Manske, K;de Almeida, GP;Kosinska, AD;Sandu, I;Schneider, A;Steinbacher, V;Teng, Y;Schnabel, J;Theis, F;Gehring, AJ;Boonstra, A;Janssen, HLA;Vandenbosch, M;Cuypers, E;Öllinger, R;Engleitner, T;Rad, R;Steiger, K;Oxenius, A;Lo, WL;Klepsch, V;Baier, G;Holzmann, B;Maini, MK;Heeren, R;Murray, PJ;Thimme, R;Herrmann, C;Protzer, U;Böttcher, JP;Zehn, D;Wohlleber, D;Lauer, GM;Hofmann, M;Luangsay, S;Knolle, PA;
    Nature
    Species: Mouse, Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  2. Guidelines for visualization and analysis of DC in tissues using multiparameter fluorescence microscopy imaging methods
    Authors: F Bayerl, DA Bejarano, G Bertacchi, AC Doffin, E Gobbini, M Hubert, L Li, P Meiser, AM Pedde, W Posch, L Rupp, A Schlitzer, M Schmitz, BU Schraml, S Uderhardt, J Valladeau-, D Wilflingse, V Zaderer, JP Böttcher
    Oncogene, 2023-01-09;0(0):e2249923.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. NK Cells Stimulate Recruitment of cDC1 into the Tumor Microenvironment Promoting Cancer Immune Control
    Authors: JP Böttcher, E Bonavita, P Chakravart, H Blees, M Cabeza-Cab, S Sammicheli, NC Rogers, E Sahai, S Zelenay, C Reis E Sou
    Cell, 2018-02-08;172(5):1022-1037.e14.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  4. Dependence of Glomerulonephritis Induction on Novel Intraglomerular Alternatively Activated Bone Marrow-Derived Macrophages and Mac-1 and PD-L1 in Lupus-Prone NZM2328 Mice
    Authors: SJ Sung, Y Ge, C Dai, H Wang, SM Fu, R Sharma, YS Hahn, J Yu, TH Le, MD Okusa, WK Bolton, JR Lawler
    J. Immunol, 2017-02-20;0(0):.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC

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Mouse Integrin  alpha E/CD103 Antibody
By Efstathios Stamatiades on 07/09/2019
Application: ICC/IF Sample Tested: Adult small intestine Species: Mouse

Cryosections from PLP immersion fixed tissue were stained for Itgae (1:100) for 4h @ RT. After washing, tissue was stained with Donkey anti-Goat alexa546 2ndary antibody for 1h at RT. CD3 (green), Itgae (red), Hoechst (blue).