Mouse Integrin beta 4/CD104 Biotinylated Antibody

Catalog #: BAF4054 Datasheet / COA / SDS

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BAF4054

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Mouse Integrin beta 4/CD104 Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Integrin beta 4/CD104 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human Integrin beta 4 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin beta 4/CD104
Asn29-Cys707
Accession # NP_001005608

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Integrin beta 4/CD104

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin beta 4/CD104

Integrin beta 4 (also GP150 and CD104) is a 150‑180 kDa member of the integrin beta family of molecules. It is found on keratinocytes, Schwann cells and perineural fibroblasts, and participates in the formation of epithelial hemidesmosomes by forming noncovalent heterodimers with Integrin alpha 6. Mature mouse Integrin beta 4 is a type I transmembrane glycoprotein that is 1774 amino acids (aa) in length. It contains a 683 aa extracellular domain (aa 29‑711) and a large 1068 aa cytoplasmic region. Three variant splice forms may exist. One shows a GlyGluHis insertion after Leu1103, a second shows a deletion of aa 1447‑1499, and a third shows the same deletion accompanied by an insertion of 65 aa after Thr1367. Over aa 29‑711, mouse beta 4 shares 88% and 96% aa identity with human and rat beta 4, respectively.

Entrez Gene IDs

3691 (Human); 192897 (Mouse); 25724 (Rat)

Alternate Names

CD104 antigen; CD104; GP150; Integrin beta 4; integrin beta-4 subunit; integrin beta-4; integrin, beta 4; ITGB4