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Mouse Integrin  beta 3/CD61 Antibody

Catalog #: MAB41182
1 Citation Datasheet / COA / SDS
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MAB41182
Detection of Integrin beta 3/CD61 in P815 Mouse Cell Line by Flow Cytometry.
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Mouse Integrin  beta 3/CD61 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Integrin  beta 3/CD61 in direct ELISA.
Source
Monoclonal Rat IgG1 Clone # 909114
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin alpha 2b and Integrin beta 3 linked heterodimer
Leu32-Arg988 (Integrin alpha 2b) & Glu26-Asp717 (Integrin beta 3)
Accession # NP_034705 (Integrin alpha 2b) & O54890 (Integrin beta 3)
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Label
Unconjugated
Purity
Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Integrin beta 3/CD61 antibody in P815 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of Integrin beta 3/CD61 in P815 Mouse Cell Line by Flow Cytometry. P815 mouse mastocytoma cell line was stained with Rat Anti-Mouse Integrin beta 3/CD61 Monoclonal Antibody (Catalog # MAB41182, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin beta 3/CD61

Integrin beta 3 (ITGB3) is also known as platelet glycoprotein IIIa (GPIIIa) and CD61. Integrins are integral cell-surface proteins known to participate in both cell adhesion and cell-surface mediated signaling. They are composed of an alpha chain and a beta chain. ITGB3 is an integrin beta chain that forms a heterodimer with alpha-IIb or alpha-V chains. It is involved in platelet aggregation, and serves as an anchor for fibrinogen, allowing platelets to form aggregates or clots. A functional absense of ITGB3 leads to Glanzmann's thrombasthenia, a condition where platelets are activated, but fail to form an aggregate. Alternatively spliced transcripts encoding different proteins have been described, in human.

Entrez Gene IDs
3690 (Human); 16416 (Mouse); 29302 (Rat); 102117791 (Cynomolgus Monkey)
Alternate Names
CD61 antigen; CD61; GP3A; GP3Aplatelet glycoprotein IIIa; GPIIIA; GPIIIaintegrin beta-3; INGRB3; Integrin beta 3; integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61); ITG B3; ITGB3; Platelet membrane glycoprotein IIIa

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Citation for Mouse Integrin  beta 3/CD61 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. The RGD-binding integrins alphavbeta6 and alphavbeta8 are receptors for mouse adenovirus-1 and -3 infection
    Authors: M Bieri, R Hendrickx, M Bauer, B Yu, T Jetzer, B Dreier, PRE Mittl, J Sobek, A Plückthun, UF Greber, S Hemmi
    PloS Pathogens, 2021-12-15;17(12):e1010083.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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