Mouse Leptin/OB Antibody

Catalog #: MAB4981 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB4981-100
MAB4981-SP

Cell Proliferation Induced by Leptin/OB and Neutralization by Mouse Leptin/OB Antibody.

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All Leptin/OB Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Leptin/OB Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Leptin/OB in direct ELISAs.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2299A

Purification

Protein A or G purified from cell culture supernatant

Immunogen

E. coli-derived mouse Leptin/OB protein
Val22-Cys167
Accession # Q544U0

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA detection antibody when paired with Rabbit Anti-Mouse Leptin/OB Monoclonal Antibody (Catalog # MAB498).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Mouse Leptin DuoSet ELISA Kit (Catalog # DY498-05) for convenient development of a sandwich ELISA or the Mouse/Rat Leptin Quantikine ELISA Kit (Catalog # MOB00B) for a complete optimized ELISA.

Neutralization

Measured by its ability to neutralize Leptin/OB-induced proliferation in the BaF3 mouse pro‑B cell line transfected with human Leptin R. The Neutralization Dose (ND50) is typically 0.1-1 µg/mL in the presence of 1 ng/mL Recombinant Mouse Leptin/OB.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Cell Proliferation Induced by Leptin/OB and Neutralization by Mouse Leptin/OB Antibody. Recombinant Mouse Leptin/OB (498-OB) stimulates proliferation in the BaF3 mouse pro‑B cell line transfected with human Leptin R in a dose-dependent manner (orange line) as measured by Resazurin (AR002). Proliferation elicited by Recombinant Mouse Leptin/OB (1 ng/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti-Mouse Leptin/OB Monoclonal Antibody (Catalog # MAB3981). The ND₅₀ is typically 0.1-1 µg/mL.

ELISA

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Mouse Leptin/OB ELISA Standard Curve. Recombinant Mouse Leptin/OB protein was serially diluted 2-fold and captured by Rabbit Anti-Mouse Leptin/OB Monoclonal Antibody (MAB498) coated on a Clear Polystyrene Microplate (DY990). Rabbit Anti-Mouse Leptin/OB Monoclonal Antibody (Catalog # MAB4981) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Leptin/OB

Leptin is a protein product of the mouse obese gene. Mice with mutations in the obese gene that block the synthesis of Leptin have been found to be obese and diabetic and to have reduced activity, metabolism and body temperature. cDNA clones encoding Leptin have been isolated from human, simian, mouse and rat cells. Mouse Leptin shares approximately 96% and 84% sequence identity with the rat and human protein, respectively. Mouse Leptin cDNA encodes a 167 amino acid residue protein with a 21 amino acid residue signal sequence that is cleaved to yield the 146 amino acid residue mature protein. The expression of Leptin mRNA has been shown to be restricted to adipose tissue.

A high-affinity receptor for Leptin (OB-R) with homology to gp130 and the G-CSF receptor has been cloned. OB-R mRNA has been shown to be expressed in the choroid plexus and in the hypothalamus. OB-R has also been identified as an isoform of B219, a sequence that is expressed in at least four isoforms in very primitive hematopoietic cell populations and in a variety of lymphohematopoietic cell lines (1-3). The possible roles of Leptin in body weight regulation, hematopoiesis and reproduction are being investigated.

References

Considine, R. and J. Caro (1996) Clinical Chemistry 42:843.
Tartaglia, L.A. et al. (1995) Cell 83:1263.
Cioffi, J.A. et al. (1996) Nature Medicine 2:585.

Entrez Gene IDs

3952 (Human); 16846 (Mouse); 25608 (Rat)

Alternate Names

FLJ94114; LEP; leptin (murine obesity homolog); leptin (obesity homolog, mouse); Leptin; OB; Obese protein; obese, mouse, homolog of; Obesity factor; OBOBS