Mouse LIFR alpha APC-conjugated Antibody Summary
Leu44-Ser828
Accession # P42703
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of LIF R alpha in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line was stained with Rat Anti-Mouse LIF Ra APC-conjugated Monoclonal Antibody (Catalog # FAB5990A, filled histogram) or isotype control antibody (Catalog # IC005A, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: LIFR alpha
Leukemia Inhibitory Factor Receptor alpha (LIF R alpha ), also known as LIFR beta (due to its participation in signal transduction) and CD118, is a 190 kDa type I transmembrane protein in the Interleukin-6 receptor family. Members of this family mediate the biological effects of Cardiotrophin-1, CLC, CNTF, IL-6, IL-11, IL-27, and Oncostatin M (1). Mature mouse LIF R alpha consists of a 785 amino acid (aa) extracellular domain (ECD) with two cytokine receptor homology domains, one WSxWS motif, and three fibronectin type III repeats, followed by a 25 aa transmembrane segment and a 239 aa cytoplasmic domain (2, 3). Within the ECD, mouse LIF R alpha shares 73% and 90% aa sequence identity with human and rat LIF R alpha, respectively. Alternative splicing generates a 90 kDa soluble form of the mouse LIF R alpha ECD (4). LIF R alpha binds the pleiotropic cytokine LIF with low affinity, and the soluble isoform retains LIF-binding activity (5). Binding affinity is increased by the ligand-induced association of LIF R alpha with the signal transducing subunit gp130 (6, 7). The LIF R alpha /gp130 receptor complex also transduces Oncostatin M signals, although LIF R alpha alone does not interact with Oncostatin M (6). gp130 associates with different ligand-specific receptors to form signaling receptor complexes for the other IL-6 family ligands (1). The CNTF receptor is a ternary complex that contains CNTF R alpha and gp130 as well as LIF R alpha (8, 9). LIF R alpha is widely expressed, and LIF induces the proliferation, differentiation, and activation of cells in many tissues (10, 11). In particular, LIF R alpha plays an important role in several aspects of early pregnancy such as blastocyst implantation in the uterus (4, 12-14).
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- Layton, M.J. et al. (1992) Proc. Natl. Acad. Sci. USA 89:8616.
- Gearing, D.P. et al. (1992) Science 255:1434.
- Giese, B. et al. (2005) J. Cell Sci. 118:5129.
- Ip, N.Y. et al. (1992) Cell 69:1121.
- Davis, S. et al. (1993) Science 260:1805.
- Metcalf, D (2003) Stem Cells 21:5.
- Kubota, Y. et al. (2008) J. Clin. Invest. 118:2393.
- Paiva, P. et al. (2009) Cytokine Growth Factor Rev. 20:319.
- Stewart, C.L. et al. (1992) Nature 359:76.
- Cheng, J.-G. et al. (2001) Proc. Natl. Acad. Sci. USA 98:8680.
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