Home / Lipocalin-2/NGAL / Mouse Lipocalin-2/NGAL Antibody

Mouse Lipocalin-2/NGAL Antibody

Catalog #: MAB1857
18 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1857-SP
MAB1857-500
MAB1857-100
Detection of Mouse Lipocalin‑2/NGAL by Western Blot.
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Citations (18)
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Mouse Lipocalin-2/NGAL Antibody Summary

Species Reactivity
Mouse
Specificity
Detects recombinant mouse (rm)Lipocalin-2 in direct ELISAs and Western blots. In these formats, does not cross-react with rhLipocalin-1 or rhLipocalin‑2.
Source
Monoclonal Rat IgG2A Clone # 228418
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Lipocalin-2
aa 21-200
Accession # P11672
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse Lipocalin-2/NGAL antibody by Western Blot. View Larger

Detection of Mouse Lipocalin‑2/NGAL by Western Blot. Western blot shows lysates of mouse spleen tissue, mouse uterus tissue, and mouse epididymis tissue. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse Lipocalin-2/NGAL Monoclonal Antibody (Catalog # MAB1857) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Lipocalin-2/NGAL at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lipocalin-2/NGAL

Lipocalin-2, also known as Neutrophil Gelatin-Associated Lipocalin (NGAL) and Siderocalin, mediates the innate immune response to bacterial infection by sequestering iron-laden siderophores.

Long Name
Neutrophil Gelatinase-associated Lipocalin
Entrez Gene IDs
3934 (Human); 16819 (Mouse); 170496 (Rat); 102137149 (Cynomolgus Monkey)
Alternate Names
24p3; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; HNL; LCN2; lipocalin 2 (oncogene 24p3); lipocalin 2; Lipocalin2; Lipocalin-2; migration-stimulating factor inhibitor; MSFI; neutrophil gelatinase-associated lipocalin; NGAL; NGALlipocalin-2; Oncogene 24p3; p25; Siderocalin; Uterocalin

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Citations for Mouse Lipocalin-2/NGAL Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

18 Citations: Showing 1 - 10
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  1. Siderocalin/Lcn2/NGAL/24p3 Does Not Drive Apoptosis Through Gentisic Acid Mediated Iron Withdrawal in Hematopoietic Cell Lines
    Authors: Colin Correnti, Vera Richardson, Allyson K. Sia, Ashok D. Bandaranayake, Mario Ruiz, Yohan Suryo Suryo Rahmanto et al.
    PLoS ONE
  2. Role of TGF-alpha in the progression of diabetic kidney disease
    Authors: Josef G. Heuer, Shannon M. Harlan, Derek D. Yang, Dianna L. Jaqua, Jeffrey S. Boyles, Jonathan M. Wilson et al.
    American Journal of Physiology-Renal Physiology
  3. Neutrophil Extracellular Traps Promote Inflammatory Responses in Psoriasis via Activating Epidermal TLR4/IL-36R Crosstalk
    Authors: Shuai Shao, Hui Fang, Erle Dang, Ke Xue, Jieyu Zhang, Bing Li et al.
    Frontiers in Immunology
  4. Activation of Constitutive Androstane Receptor Ameliorates Renal Ischemia-Reperfusion–Induced Kidney and Liver Injury
    Authors: You-Jin Choi, Dong Zhou, Anne Caroline S. Barbosa, Yongdong Niu, Xiudong Guan, Meishu Xu et al.
    Molecular Pharmacology
  5. The contribution of IL-17A-dependent low LCN2 levels to Helicobacter pylori infection: Insights from clinical and experimental studies
    Authors: Cheng, M;Zheng, Y;Fan, Y;Yan, P;Zhao, W;
    International immunopharmacology
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  6. Critical changes in hypothalamic gene networks in response to pancreatic cancer as found by single-cell RNA sequencing
    Authors: C Huisman, MA Norgard, PR Levasseur, SM Krasnow, MGP van der Wi, B Olson, DL Marks
    Molecular Metabolism, 2022-01-11;0(0):101441.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Macrophage-Derived Iron-Bound Lipocalin-2 Correlates with Renal Recovery Markers Following Sepsis-Induced Kidney Damage
    Authors: C Mertens, L Kuchler, A Sola, R Guiteras, S Grein, B Brüne, A von Knethe, M Jung
    Int J Mol Sci, 2020-10-13;21(20):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Immunoprecipitation
  8. Combination of In Situ Lcn2 pRNA-RNAi Nanotherapeutics and iNSC Transplantation Ameliorates Experimental SCI in Mice
    Authors: A Braga, S Bandiera, J Verheyen, R Hamel, C Rutigliani, F Edenhofer, JA Smith, S Pluchino
    Mol. Ther., 2020-08-05;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  9. The Fibrin Cleavage Product B?15-42 Channels Endothelial and Tubular Regeneration in the Post-acute Course During Murine Renal Ischemia Reperfusion Injury
    Authors: D Fischer, C Seifen, P Baer, M Jung, C Mertens, B Scheller, K Zacharowsk, R Hofmann, TJ Maier, A Urbschat
    Front Pharmacol, 2018-04-27;9(0):369.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. RNA Nanotherapeutics for the Amelioration of Astroglial Reactivity
    Authors: JA Smith, A Braga, J Verheyen, S Basilico, S Bandiera, C Alfaro-Cer, L Peruzzotti, D Shu, F Haque, P Guo, S Pluchino
    Mol Ther Nucleic Acids, 2017-11-24;10(0):103-121.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: ICC
  11. Neutrophil gelatinase?associated lipocalin as a potential novel biomarker for ventilator?associated lung injury
    Authors: R Xiao, R Chen
    Mol Med Rep, 2017-04-07;15(6):3535-3540.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  12. Lipocalin-2 Promotes Pancreatic Ductal Adenocarcinoma by Regulating Inflammation in the Tumor Microenvironment
    Authors: S Gomez-Chou, A Swidnicka-, N Badi, M Chavez-Tom, GB Lesinski, T Bekaii-Saa, MR Farren, TA Mace, C Schmidt, Y Liu, D Deng, RF Hwang, L Zhou, TT Moore, D Chatterjee, H Wang, X Leng, RB Arlinghaus, CD Logsdon, Z Cruz-Monse
    Cancer Res, 2017-03-01;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  13. Differential role of lipocalin 2 during immune complex-mediated acute and chronic inflammation in mice.
    Authors: Shashidharamurthy R, Machiah D, Aitken J, Putty K, Srinivasan G, Chassaing B, Parkos C, Selvaraj P, Vijay-Kumar M
    Arthritis Rheum, 2013-04-01;65(4):1064-73.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  14. Molecular MRI of murine atherosclerotic plaque targeting NGAL: a protein associated with unstable human plaque characteristics.
    Authors: te Boekhorst BC, Bovens SM, Hellings WE, van der Kraak PH, van de Kolk KW, Vink A, Moll FL, van Oosterhout MF, de Vries JP, Doevendans PA, Goumans MJ, de Kleijn DP, van Echteld CJ, Pasterkamp G, Sluijter JP
    Cardiovasc. Res., 2010-10-28;89(3):680-8.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  15. Absence of functional Hfe protects mice from invasive Salmonella enterica serovar Typhimurium infection via induction of lipocalin-2.
    Authors: Nairz M, Theurl I, Schroll A, Theurl M, Fritsche G, Lindner E, Seifert M, Crouch ML, Hantke K, Akira S, Fang FC, Weiss G
    Blood, 2009-08-21;114(17):3642-51.
    Species: Mouse
    Sample Types: Serum
    Applications: Immunoprecipitation
  16. Interferon-gamma limits the availability of iron for intramacrophage Salmonella typhimurium.
    Authors: Nairz M, Fritsche G, Brunner P, Talasz H, Hantke K, Weiss G
    Eur. J. Immunol., 2008-07-01;38(7):1923-36.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  17. Lcn2 mediates adipocyte-muscle-tumor communication and hypothermia in pancreatic cancer cachexia
    Authors: Mengistu Lemecha, Jaya Prakash Chalise, Yuki Takamuku, Guoxiang Zhang, Takahiro Yamakawa, Garrett Larson et al.
    Molecular Metabolism
  18. Dysregulated Microbial Fermentation of Soluble Fiber Induces Cholestatic Liver Cancer.
    Authors: Singh V, Yeoh BS, Chassaing B et al.
    Cell.

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