Mouse Ly-6G/Ly-6C (Gr-1) Biotinylated Antibody

Catalog #: BAM1037
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BAM1037

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Mouse Ly-6G/Ly-6C (Gr-1) Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Ly-6G/Ly-6C (Gr-1). Weak cross-reactivity with Ly-6C is observed.

Source

Monoclonal Rat IgG_2B Clone # RB6-8C5

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

The immunogen for this antibody is Ly-6G (Gr-1, Gr1).

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

Mouse splenocytes

Immunohistochemistry

Nitta, H. et al. (1997) Cell Vision 4:73.

Immunoprecipitation

Conlan, J.W. and R.J. North (1994) J. Exp. Med. 179:259.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Ly-6G/Ly-6C (Gr-1) in Mouse Spleen. Ly-6G/Ly-6C (Gr-1) was detected in immersion fixed frozen sections of mouse spleen using Rat Anti-Mouse Ly-6G/Ly-6C (Gr-1) Biotinylated Monoclonal Antibody (Catalog # BAM1037) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ly-6G/Ly-6C (Gr-1)

Gr-1, also called Ly-6G, is a myeloid differentiation antigen that is a member of the Ly-6 family. The RB6‑8C5 antibody recognizes Gr-1/Ly-6G, and also reacts weakly with Ly-6C-transfected EL4 cells (1, 2). In the periphery, this antibody specifically recognized granulocytes (3, 4).

References

Spangrude, G.J. et al. (1988) Science 241:58
Fleming, T.J. et al. (1993) J. Immunol. 151:2399
Lewinsohn, D.M. et al. (1987) J. Immunol. 147:22
Lagasse, E. and I. L. Weissman (1996) J. Immunol. Methods 197:139

Long Name

A Myeloid Differentiation Antigen

Entrez Gene IDs

546644 (Mouse)

Alternate Names

C6orf23; G6D; Ly-6C; LY6-D; Ly6G; Ly-6G; Ly-6G/Ly-6C (Gr-1); LY6G6D; lymphocyte antigen 6 family member G6D; MEGT1; NG25

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Product Datasheet

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Related Research Areas

Citation for Mouse Ly-6G/Ly-6C (Gr-1) Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Oral chymase inhibitor SUN13834 ameliorates skin inflammation as well as pruritus in mouse model for atopic dermatitis.
Authors: Terakawa M, Fujieda Y, Tomimori Y, Muto T, Tanaka T, Maruoka H, Nagahira K, Ogata A, Nakatsuka T, Fukuda Y
Eur. J. Pharmacol., 2008-10-29;601(1):186-91.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P