Mouse MAdCAM-1 Antibody Summary
Gln22-Thr365, Predicted
Accession # NP_038619
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Mouse MAdCAM‑1 by Western Blot. Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse MAdCAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF993) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for MAdCAM-1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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MAdCAM‑1 in Mouse Intestine. MAdCAM-1 was detected in perfusion fixed frozen sections of mouse intestine using Goat Anti-Mouse MAdCAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF993) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the endothelial cells of blood capillaries in intestinal villi. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MAdCAM-1
Mucosal addressin cell adhesion molecule-1 (MAdCAM-1) is an immunoglobulin (Ig) cell adhesion molecule family member. In addition to Ig domains, it contains a mucin-like domain and a membrane proximal domain with similarity to IgA. MAdCAM-1 is involved in lymphocyte homing to mucosal sites and is expressed on high endothelial venules (HEV) of both mesenteric lymph nodes and Peyer’s patches. It has also been found to be expressed on sinus-lining cells of the spleen. The integrin, alpha 4 beta 7, has been shown to function as the MAdCAM-1 receptor. The Ig domains of MAdCAM-1 have been found to be critical to alpha 4 beta 7 binding. The mucin domain has been shown to have activity in L-Selectin binding. MAdCAM-1 expression has been demonstrated to be up-regulated by TNF-alpha and IL-1 beta. MAdCAM-1 appears to play a role in inflammatory bowel disease (IBD) as its expression is highly up-regulated in IBD and most likely serves to recruit alpha 4 beta 7-expressing lymphocytes to the region. In vivo studies involving nonobese diabetic (NOD) mice have also suggested that MAdCAM-1/ alpha 4 beta 7 interaction plays a role in diabetes development in this model. Mouse MAdCAM-1 is a 405 amino acid (aa) residue protein with a 21 aa signal sequence, a 344 aa extracellular domain, a 20 aa transmembrane domain and a 20 aa cytoplasmic domain.
- Briskin, M.J. et al. (1993) Nature 363:461.
- Yang, X.D. et al. (1997) Diabetes 46:1542.
- Sampaio, S.O. et al. (1995) J. Immunol. 155:2477.
- Kraal, G. et al. (1995) Am. J. Pathol. 147:763.
- Berg, E.L. et al. (1993) Nature 366:695.
- Takeuchi, M. and V.R. Baichwal (1995) Proc. Natl. Acad. Sci. USA 92:3561.
Product Datasheets
Citations for Mouse MAdCAM-1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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TRIM59 loss in M2 macrophages promotes melanoma migration and invasion by upregulating MMP-9 and Madcam1
Authors: Yuan Tian, Yantong Guo, Pei Zhu, Dongxu Zhang, Shanshan Liu, Mengyan Tang et al.
Aging (Albany NY)
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T Cell Recruitment to the Intestinal Stem Cell Compartment Drives Immune-Mediated Intestinal Damage after Allogeneic Transplantation
Authors: Ya-Yuan Fu, Anastasiya Egorova, Catherine Sobieski, Jason Kuttiyara, Marco Calafiore, Shuichiro Takashima et al.
Immunity
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Targeting improves MSC treatment of inflammatory bowel disease.
Authors: Ko IK, Kim BG, Awadallah A
Mol. Ther., 2010-04-13;18(7):1365-72.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization
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Tissues fixed in 10% Formalin and embedded in paraffin blocks. Antigen retrieval in Tris-Base EDTA buffer pH 9.0. Incubated overnight with MAdCAM-1 at a concentration of 5ug/ml. Used CTS008 (Anti-Goat HRP-DAB cell and tissue staining kit) and saw signal in endothelial cells with no background.
