Mouse MAdCAM-1 Biotinylated Antibody

Catalog #: BAF993 Datasheet / COA / SDS

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BAF993

All MAdCAM-1 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse MAdCAM-1 Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse MAdCAM-1 in ELISAs and Western blots. In sandwich immunoassays, less than 0.3% cross‑reactivity with recombinant mouse (rm) ALCAM, recombinant human (rh) BCAM, rhEpCAM, rmICAM-1, rmICAM-2, rhICAM-3, and rmVCAM-1 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse MAdCAM-1
Gln22-Thr365, predicted
Accession # NP_038619

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse MAdCAM‑1 Fc Chimera (Catalog # 993-MC)

Mouse MAdCAM-1 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Mouse MAdCAM‑1 Antibody (Catalog # MAB9931)

Standard: Recombinant Mouse MAdCAM-1 Fc Chimera Protein, CF (Catalog # 993-MC)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MAdCAM-1

Mucosal addressin cell adhesion molecule-1 (MAdCAM-1) is an immunoglobulin (Ig) cell adhesion molecule family member. In addition to Ig domains, it contains a mucin-like domain and a membrane proximal domain with similarity to IgA. MAdCAM-1 is involved in lymphocyte homing to mucosal sites and is expressed on high endothelial venules (HEV) of both mesenteric lymph nodes and Peyer’s patches. It has also been found to be expressed on sinus-lining cells of the spleen. The integrin, alpha ₄ beta ₇, has been shown to function as the MAdCAM-1 receptor. The Ig domains of MAdCAM-1 have been found to be critical to alpha ₄ beta ₇ binding. The mucin domain has been shown to have activity in L-Selectin binding. MAdCAM-1 expression has been demonstrated to be up-regulated by TNF-alpha and IL-1 beta. MAdCAM-1 appears to play a role in inflammatory bowel disease (IBD) as its expression is highly up-regulated in IBD and most likely serves to recruit alpha ₄ beta ₇-expressing lymphocytes to the region. In vivo studies involving nonobese diabetic (NOD) mice have also suggested that MAdCAM-1/ alpha ₄ beta ₇ interaction plays a role in diabetes development in this model. Mouse MAdCAM-1 is a 405 amino acid (aa) residue protein with a 21 aa signal sequence, a 344 aa extracellular domain, a 20 aa transmembrane domain and a 20 aa cytoplasmic domain.

References

Briskin, M.J. et al. (1993) Nature 363:461.
Yang, X.D. et al. (1997) Diabetes 46:1542.
Sampaio, S.O. et al. (1995) J. Immunol. 155:2477.
Kraal, G. et al. (1995) Am. J. Pathol. 147:763.
Berg, E.L. et al. (1993) Nature 366:695.
Takeuchi, M. and V.R. Baichwal (1995) Proc. Natl. Acad. Sci. USA 92:3561.

Long Name

Mucosal Addressin Cell Adhesion Molecule 1

Entrez Gene IDs

8174 (Human); 17123 (Mouse)

Alternate Names

hMAdCAM-1; MACAM1; MAdCAM1; MAdCAM-1; mucosal addressin cell adhesion molecule 1; mucosal addressin cell adhesion molecule-1; mucosal vascular addressin cell adhesion molecule 1