Mouse Mast Cell Protease-1/Mcpt1 Antibody

Catalog #: AF5146
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5146
AF5146-SP

Detection of Mouse Mast Cell Protease‑1/Mcpt1 by Western Blot.

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All Mast Cell Protease-1/Mcpt1 Antibodies

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Mast Cell Protease-1/Mcpt1 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Mast Cell Protease‑1/Mcpt1 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant mouse (rm) Mcpt6, rmMcpt7, and rmMcpt11 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Mast Cell Protease‑1/Mcpt1
Glu19-Leu246
Accession # P11034

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Simple Western

50 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse Mast Cell Protease‑1/Mcpt1 by Western Blot. Western blot shows lysates of mouse small intestine tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Mast Cell Protease-1/Mcpt1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5146) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Mast Cell Protease-1/Mcpt1 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western

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Detection of Mouse Mast Cell Protease‑1/Mcpt1 by Simple Western^TM. Simple Western lane view shows lysates of mouse small intestine tissue, loaded at 0.2 mg/mL. A specific band was detected for Mast Cell Protease-1/Mcpt1 at approximately 34 kDa (as indicated) using 50 µg/mL of Sheep Anti-Mouse Mast Cell Protease-1/Mcpt1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5146) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Mast Cell Protease-1/Mcpt1

Mast cell protease-1 (Mcpt1), also known as beta ‑chymase, is a member of the Chymase family of chymotrypsin-like serine proteases (1). mMcpt1 is a mast cell protease predominantly expressed in intestinal mucosal mast cells where it promotes mucosal permeability in intestinal allergic hypersensitivity reactions (2). Its activation is completed by the removal of a two residue N‑terminal propeptide by a dipeptidyl peptidase (Cathepsin C) (3). Like human alpha ‑Chymase, Mcpt1 is capable of the conversion of angiotensin I to angiotensin II, which plays a key role in the regulation of arterial pressure (4). Studies have shown that specific chymase inhibitors are able to diminish the development of abdominal aortic aneurysm and reduce the adhesion formation after cardiac surgery in hamsters (5, 6). Therefore, the development of specific inhibitors of chymase activity has been a pharmacologic strategy to develop therapeutic agents.

References

Caughey, G.H. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.), p. 1531 Academic Press, San Diego.
Wastling, J.M. et al. (1998) Am. J. Pathol. 153:491.
Murakami, M. et al. (1995) J. Biol. Chem. 270:2218.
Saito, K. et al. (2003) Biochem. Biophys. Res. Commun. 302:773.
Tsunemi, K. et al. (2004) J. Pharmacol. Exp. Ther. 309:879.
Soga, Y. et al. (2004) J. Thorac. Cardiovasc. Surg. 127:72.

Entrez Gene IDs

17224 (Mouse); 29265 (Rat)

Alternate Names

AV080368; Mast Cell Protease1; Mast Cell Protease-1; Mcp1; Mcp-1; MMPC-1

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Product Datasheet

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Citation for Mouse Mast Cell Protease-1/Mcpt1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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