Mouse Mrc2 Antibody

Catalog #: AF4789
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4789
AF4789-SP

Detection of Mouse Mrc2 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Mrc2 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Mrc2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross-reactivity with recombinant human Mrc2 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Mrc2 Ala31-Ala1413 Accession # AAI16643

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Immunogen affinity purified

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse Mrc2 by Western Blot. Western blot shows lysates of mouse heart, lung, and kidney tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Mrc2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4789) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Mrc2 at approximately 170-180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Background: Mrc2

Mrc2 (macrophage mannose receptor 2; also uPARAP/Endo180) is a 180 kDa member of the mannose receptor family. It is found on fibroblasts, macrophages and endothelial cells. Mrc2 binds and internalizes glycoproteins plus collagen types I and IV. Mouse Mrc2 is a type I transmembrane glycoprotein that is 1479 amino acids (aa) in length. It possesses a 1383 aa extracellular domain (ECD) (aa 31-1479) and a short 45 aa cytoplasmic tail. The ECD contains eight C-type lectin domains plus one fibronectin type II domain (aa 181-229) that binds collagen. There are two splice variants, one which shows an eight aa substitution for aa 173-1479, and a second that shows a 72 aa substitution for aa 982-1479. Over aa 311-413, mouse Mrc2 shares 91% and 97% aa identity with human and rat Mrc2, respectively.

Long Name

Mannose Receptor, C-type 2

Entrez Gene IDs

9902 (Human); 17534 (Mouse); 498011 (Rat)

Alternate Names

CD280 antigen; CD280; CD280C-type mannose receptor 2; CLEC13E; CLEC13EUPAR-associated protein; Endo180; ENDO180C-type lectin domain family 13 member E; Endocytic receptor 180; KIAA0709FLJ35911; Macrophage mannose receptor 2; mannose receptor, C type 2; Mrc2; uPARAP; UPARAPendocytic receptor (macrophage mannose receptor family); urokinase plasminogen activator receptor-associated protein; Urokinase receptor-associated protein; Urokinase-type plasminogen activator receptor-associated protein

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Citation for Mouse Mrc2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

The proteasome modulates endocytosis specifically in glomerular cells to promote kidney filtration
Authors: Sachs, W;Blume, L;Loreth, D;Schebsdat, L;Hatje, F;Koehler, S;Wedekind, U;Sachs, M;Zieliniski, S;Brand, J;Conze, C;Florea, BI;Heppner, F;Krüger, E;Rinschen, MM;Kretz, O;Thünauer, R;Meyer-Schwesinger, C;
Nature communications
Species: Murine polyomavirus strain A3
Sample Types: Cell Lysates, Whole Tissue
Applications: Western Blot, Immunohistochemistry