Mouse MyD88 Antibody

Catalog #: MAB3109
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3109
MAB3109-SP

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Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse MyD88 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse MyD88 in direct ELISAs.

Source

Monoclonal Rat IgG_2A Clone # 316902

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse MyD88
Met1-Pro296
Accession # Q3U7M4

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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MyD88 in RAW 264.7 Mouse Cell Line. MyD88 was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Rat Anti-Mouse MyD88 Monoclonal Antibody (Catalog # MAB3109) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL014) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MyD88

MyD88 was first identified as a myeloid differentiation primary response protein. It contains a cytoplasmic Toll/interleukin-1 receptor (TIR) domain and is classified as a member of the TIR superfamily. MyD88 is expressed in many adult tissues including heart, kidney and liver tissue and in monocyte, T, B, NK and dendritic cells.

Long Name

Myeloid Differentiation Primary Response Gene 88

Entrez Gene IDs

4615 (Human); 17874 (Mouse)

Alternate Names

MyD88; MYD88D; myeloid differentiation primary response gene (88); myeloid differentiation primary response protein MyD88

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citations for Mouse MyD88 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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HMGB1 is an early and critical mediator in an animal model of uveitis induced by IRBP-specific T cells.
Authors: Jiang G, Sun D, Yang H, Lu Q, Kaplan H, Shao H
J Leukoc Biol, 2013-12-27;95(4):599-607.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Bacterial recognition by TLR7 in the lysosomes of conventional dendritic cells.
Authors: Mancuso G, Gambuzza M, Midiri A, Biondo C, Papasergi S, Akira S, Teti G, Beninati C
Nat. Immunol., 2009-06-01;10(6):587-94.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC