Mouse Nanog Alexa Fluor® 488-conjugated Antibody

Catalog #: IC2729G Datasheet / COA / SDS

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IC2729G

Detection of Nanog in D3 Mouse Cell Line by Flow Cytometry.

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Mouse Nanog Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Nanog in Western blots. In this format, approximately 50% cross-reactivity with recombinant human Nanog is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse Nanog
Trp154-Leu262
Accession # Q80Z64

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Purity

Antigen Affinity-purified

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

See below

Intracellular Staining by Flow Cytometry

5 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of Nanog antibody in D3 Mouse Cell Line antibody by Flow Cytometry.

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Detection of Nanog in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line either untreated (dark orange filled histogram) or treated with retinoic acid for 3 days (light orange filled histogram) was stained with Goat Anti-Mouse Nanog Alexa Fluor® 488-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC2729G) or isotype control antibody (Catalog # IC108G, blue open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Immunocytochemistry

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Nanog in D3 Mouse Embryonic Stem Cells. Nanog was detected in immersion fixed D3 mouse embryonic stem cell line on irradiated mouse embryonic fibroblasts using Goat Anti-Mouse Nanog Alexa Fluor® 488‑conjugated Antigen Affinity-purified Polyclonal Antibody (green; Catalog # IC2729G) at 10 µg/mL for 3 hours at room temperature. Cells were counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Nanog

Nanog is a member of the homeobox family of DNA binding transcription factors that has been shown to maintain pluripotency of embryonic stem cells. Its expression is high in undifferentiated embryonic stem cells and is down-regulated during embryonic stem cell differentiation, concomitant with loss of pluripotency (1‑3).

References

Mitsui, K. et al. (2003) Cell 11:631.
Chambers, I. et al. (2003) Cell 113:643.
Hart, A.H. et al. (2004) Dev. Dyn. 230:187.

Long Name

Nanog Homeobox

Entrez Gene IDs

79923 (Human); 71950 (Mouse)

Alternate Names

FLJ12581; FLJ40451; hNanog; homeobox protein NANOG; Homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; Nanog homeobox; Nanog

Product Datasheets

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Product Specific Notices

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.