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Mouse Nanog Antibody

Catalog #: AF2729
20 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2729
AF2729-SP
Detection of Nanog in D3 Mouse Cell Line by Flow Cytometry.
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Product Details
Citations (20)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Nanog Antibody Summary

Species Reactivity
Mouse
Specificity
Detects recombinant mouse Nanog in Western blots. In this format, approximately 50% cross-reactivity with recombinant human Nanog is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse Nanog
Trp154-Leu262
Accession # Q80Z64
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
Yang, W. et al. (2014) Nat. Comm. 5:3818
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
See below
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of Nanog antibody in D3 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of Nanog in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line was stained with Goat Anti-Mouse Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2729, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).

Immunocytochemistry Nanog antibody in D3 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

Nanog in D3 Mouse Cell Line. Nanog was detected in immersion fixed D3 mouse embryonic stem cell line using Goat Anti-Mouse Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2729) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nanog

Nanog is a member of the homeobox family of DNA binding transcription factors that has been shown to maintain pluripotency of embryonic stem cells. Its expression is high in undifferentiated embryonic stem cells and is down-regulated during embryonic stem cell differentiation, concomitant with loss of pluripotency
(1-3).

References
  1. Mitsui, K. et al. (2003) Cell 11:631.
  2. Chambers, I. et al. (2003) Cell 113:643.
  3. Hart, A.H. et al. (2004) Dev. Dyn. 230:187.
Long Name
Nanog Homeobox
Entrez Gene IDs
79923 (Human); 71950 (Mouse)
Alternate Names
FLJ12581; FLJ40451; hNanog; homeobox protein NANOG; Homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; Nanog homeobox; Nanog

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Citations for Mouse Nanog Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
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  1. Mutations in the transcriptional regulator MeCP2 severely impact key cellular and molecular signatures of human astrocytes during maturation
    Authors: J Sun, S Osenberg, A Irwin, LH Ma, N Lee, Y Xiang, F Li, YW Wan, IH Park, M Maletic-Sa, N Ballas
    Cell Reports, 2023-01-05;42(1):111942.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Impaired stem cell differentiation and somatic cell reprogramming in DIDO3 mutants with altered RNA processing and increased R-loop levels
    Authors: A Fütterer, A Talavera-G, T Pons, J de Celis, J Gutiérrez, V Domínguez, C Martínez-A
    Cell Death & Disease, 2021-06-21;12(7):637.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC
  3. Hemi-methylated CpG sites connect Dnmt1-knockdown-induced and Tet1-induced DNA demethylation during somatic cell reprogramming
    Authors: S He, F Wang, Y Zhang, J Chen, L Liang, Y Li, M Zhang, X Yang, H Pang, Y Li, X Huang, D Qin, D Pei, H Sun, H Zheng
    Cell Discov, 2019-02-12;5(0):11.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Coordinated Control of mRNA and rRNA Processing Controls Embryonic Stem Cell Pluripotency and Differentiation
    Authors: NS Corsini, AM Peer, P Moeseneder, M Roiuk, TR Burkard, HC Theussl, I Moll, JA Knoblich
    Cell Stem Cell, 2018-04-05;22(4):543-558.e12.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  5. Mediator complex component MED13 regulates zygotic genome activation and is required for postimplantation development in the mouse
    Authors: YL Miao, A Gambini, Y Zhang, E Padilla-Ba, WN Jefferson, ML Bernhardt, W Huang, L Li, CJ Williams
    Biol. Reprod., 2018-04-01;0(0):.
    Species: Mouse
    Sample Types: Embryo
    Applications: IHC
  6. HIF1? regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia
    Authors: P Wang, C Lan, S Xiong, X Zhao, Y Shan, R Hu, W Wan, S Yu, B Liao, G Li, J Wang, D Zou, B Chen, H Feng, N Wu
    Oncotarget, 2017-04-25;8(17):28074-28092.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  7. ROCK inhibitor primes human induced pluripotent stem cells to selectively differentiate towards mesendodermal lineage via epithelial-mesenchymal transition-like modulation
    Stem Cell Res, 2016-08-04;17(2):222-227.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  8. Lysine-specific histone demethylase 1 inhibition promotes reprogramming by facilitating the expression of exogenous transcriptional factors and metabolic switch
    Sci Rep, 2016-08-02;6(0):30903.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC-Fr
  9. Embryonic Stem Cell Growth Factors Regulate eIF2alpha Phosphorylation.
    Authors: Friend K, Brooks H, Propson N, Thomson J, Kimble J
    PLoS ONE, 2015-09-25;10(9):e0139076.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: IHC, Western Blot
  10. Cyclin-dependent kinase-mediated Sox2 phosphorylation enhances the ability of Sox2 to establish the pluripotent state.
    Authors: Ouyang J, Yu W, Liu J, Zhang N, Florens L, Chen J, Liu H, Washburn M, Pei D, Xie T
    J Biol Chem, 2015-07-02;290(37):22782-94.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  11. FBXL10 protects Polycomb-bound genes from hypermethylation.
    Authors: Boulard M, Edwards J, Bestor T
    Nat Genet, 2015-04-06;47(5):479-85.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC
  12. Acute loss of Cited2 impairs Nanog expression and decreases self-renewal of mouse embryonic stem cells.
    Authors: Kranc K, Oliveira D, Armesilla-Diaz A, Pacheco-Leyva I, Catarina Matias A, Luisa Escapa A, Subramani C, Wheadon H, Trindade M, Nichols J, Kaji K, Enver T, Braganca J
    Stem Cells, 2015-03-01;33(3):699-712.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  13. Loss of tenomodulin results in reduced self-renewal and augmented senescence of tendon stem/progenitor cells.
    Authors: Alberton P, Dex S, Popov C, Shukunami C, Schieker M, Docheva D
    Stem Cells Dev, 2014-12-10;24(5):597-609.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC
  14. The histone H2A deubiquitinase Usp16 regulates embryonic stem cell gene expression and lineage commitment.
    Authors: Yang, Wei, Lee, Yun-Hwa, Jones, Amanda E, Woolnough, Jessica, Zhou, Dewang, Dai, Qian, Wu, Qiang, Giles, Keith E, Townes, Tim M, Wang, Hengbin
    Nat Commun, 2014-05-02;5(0):3818.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  15. STAT3 phosphorylation at tyrosine 705 and serine 727 differentially regulates mouse ESC fates.
    Authors: Huang G, Yan H, Ye S, Tong C, Ying Q
    Stem Cells, 2014-05-01;32(5):1149-60.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  16. Generation of transgene-free mouse induced pluripotent stem cells using an excisable lentiviral system.
    Authors: Varga E, Nemes C, Davis R, Ujhelly O, Klincumhom N, Polgar Z, Muenthaisong S, Pirity M, Dinnyes A
    Exp Cell Res, 2014-02-18;322(2):335-44.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC-P
  17. Gbx2, a LIF/Stat3 target, promotes reprogramming to and retention of the pluripotent ground state.
    Authors: Tai, Chih-I, Ying, Qi-Long
    J Cell Sci, 2013-01-23;126(0):1093-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  18. Small RNA-mediated regulation of iPS cell generation.
    Authors: Li Z, Yang CS, Nakashima K, Rana TM
    EMBO J., 2011-02-01;30(5):823-34.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  19. LIF-independent JAK signalling to chromatin in embryonic stem cells uncovered from an adult stem cell disease.
    Authors: Griffiths DS, Li J, Dawson MA, Trotter MW, Cheng YH, Smith AM, Mansfield W, Liu P, Kouzarides T, Nichols J, Bannister AJ, Green AR, Gottgens B
    Nat. Cell Biol., 2010-12-12;13(1):13-21.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  20. Klf5 is involved in self-renewal of mouse embryonic stem cells.
    Authors: Parisi S, Passaro F, Aloia L, Manabe I, Nagai R, Pastore L, Russo T
    J. Cell. Sci., 2008-07-24;121(0):2629-34.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells, Whole Tissue
    Applications: ChIP, ICC, IHC

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